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Improvement on Yield of Extracts from Byproducts of Alaska Pollock Theragra chalcogramma and Sea Tangle Laminaria japonica using Commercial Enzymes and Its Food Component Characterization

상업적 효소를 이용한 명태(Theragra chalcogramma) 두부 및 정형 다시마(Laminaria japonica) 부산물 유래 고압 추출물의 수율개선 및 이의 식품성분 특성

  • Noh, Yuni (Department of Seafood Science and Technology/Institute of Marine Industry, Gyeongsang National University) ;
  • Park, Kwon Hyun (Department of Seafood Science and Technology/Institute of Marine Industry, Gyeongsang National University) ;
  • Lee, Ji Sun (Department of Seafood Science and Technology/Institute of Marine Industry, Gyeongsang National University) ;
  • Kim, Hyeon Jeong (Department of Seafood Science and Technology/Institute of Marine Industry, Gyeongsang National University) ;
  • Kim, Min Ji (Department of Seafood Science and Technology/Institute of Marine Industry, Gyeongsang National University) ;
  • Kim, Ki Hyun (Department of Seafood Science and Technology/Institute of Marine Industry, Gyeongsang National University) ;
  • Kim, Jeong Gyun (Department of Seafood Science and Technology/Institute of Marine Industry, Gyeongsang National University) ;
  • Heu, Min Soo (Department of Food Science and Nutrition, Gyeongsang National University) ;
  • Kim, Jin-Soo (Department of Seafood Science and Technology/Institute of Marine Industry, Gyeongsang National University)
  • 노윤이 (경상대학교 해양식품공학과/해양산업연구소) ;
  • 박권현 (경상대학교 해양식품공학과/해양산업연구소) ;
  • 이지선 (경상대학교 해양식품공학과/해양산업연구소) ;
  • 김현정 (경상대학교 해양식품공학과/해양산업연구소) ;
  • 김민지 (경상대학교 해양식품공학과/해양산업연구소) ;
  • 김기현 (경상대학교 해양식품공학과/해양산업연구소) ;
  • 김정균 (경상대학교 해양식품공학과/해양산업연구소) ;
  • 허민수 (경상대학교 식품영양학과) ;
  • 김진수 (경상대학교 해양식품공학과/해양산업연구소)
  • Received : 2012.05.04
  • Accepted : 2012.10.30
  • Published : 2013.02.28

Abstract

This study was conducted to improve the yield of extracts from Alaska pollock Theragra chalcogramma head and sea tangle Laminaria japonica byproducts using various commercial enzymes, such as Alcalase, Flavourzyme, Neutrase (NH), and Protamex. Among the enzymatic hydrolysates, the yield was highest in hydrolysate incubated with NH for 4 h. NH-treated hydrolysates (NHH) also improved functional properties, such as angiotensin-I converting enzyme (ACE) inhibitory activity and 2,2-diphenyl-1-picryldrazyl (DPPH) radical scavenging activity, as compared to extracts from Alaska pollock head and sea tangle byproducts. Total free amino acid and taste values of NHH were 379.7 mg/100 mL and 24.03, respectively, after digestion for 4 h. These values are 2.2-fold and 1.9-fold higher compared with those of water soluble fractions extracted from Alaska pollock head and non-forming sea tangle, respectively. According to the taste value results, the major taste-active compounds among free amino acids of NHH were glutamic acid and aspartic acid. These results suggest that NHH can be used as an ingredient for natural seasoning preparation.

Keywords

References

  1. Blois MS. 1958. Antioxidant determination by the use of a stable free radical. Nature 181, 1199-1200. https://doi.org/10.1038/1811199a0
  2. Byun HG and Kim SK. 2001. Purification and characterization of angiotensin I converting enzyme (ACE) inhibitory peptides from Alaska pollack (Theragra chalcogramma) skin. Process Biochem 36, 1155-1162. http://dx.doi.org/10.4014/jmb.1109.09015
  3. Cha YJ, Kim H, Jang SM and Park JY. 1999. Identification of aroma-active compounds in Korean salt-fermented fishes by aroma extract dilution analysis. 1. Aroma-active components in salt- fermented anchovy on the market. J Korean Soc Food Sci Nutr 28, 312-318.
  4. Fu H, Katsumura Y, Lin M, Muroya Y, Hata K, Fujii K, Akinari Y and Yoshiko H. 2009. Free radical scavenging and radioprotective effect of carnosine and anserine. Radiation Physics Chem 78, 1192-1197. https://doi.org/10.1016/j.radphyschem.2009.07.023
  5. Heu MS, Kim HS, Ham JS, Park SH, Kim HS, Kang KT, Jee SJ, Lee JH and Kim JS. 2008. Preparation and quality characteristics of seasoned and dried fish slice products using rainbow trout (Onchorhynchus mykiss). J Korean Soc Food Sci Nutr 37, 348-356. https://doi.org/10.3746/jkfn.2008.37.3.348
  6. Heu MS, Park SH, Kim HS, Jee SJ, Lee JH, Kim HJ, Han BW and Kim JS. 2007. Improvement on the functional properties of Gomtang-like product from salmon frame using commercial enzymes. J Korea Soc Food Sci Nutr 36, 1596-1603. http://dx.doi.org/10.3746/jkfn.2007.36.12.1596
  7. Horiuchi M, Fujimura KI, Terashima T and Iso T. 1982. Method for determination of angiotensin-converting enzyme activity in blood tissue by high-performance liquid chromatography. J Chromatogr 233, 123-130. https://doi.org/10.1016/S0378-4347(00)81738-7
  8. Jo CR, Kim DH, Lee WD, Lee JJ and Byun MW. 2003. Application of gamma irradiation on manufacturing Changran jeotgal (aged and seasoned intestine of Alaska pollack): Microbiological and sensory characteristics. J Korean Soc Food Sci Nutr 32, 673-678. https://doi.org/10.3746/jkfn.2003.32.5.673
  9. Kang GT. 2007. Development of functional oyster drink using liquid byproducts from canned oyster. MS thesis. Gyeongsang National University, Tongyeong, Korea.
  10. Kato H, Rhue MR and Nishimura T. 1989. Role of free amino acids and peptides in food taste. In Flavor Chemistry Trends and Developments. American Chemical Society, Washington, DC, USA, 158-174.
  11. Kim JS, Heu MS and Ha JH. 2007. Fundamentals and Applications of Seafood Processing. Hyoil Publishing Co., Seoul, Korea. 41-45, 308-314.
  12. Kim MJ, Nahmgung B, Kim BN, Lee SJ, Kim CJ, Cho YJ and Kim CT. 2009. Preparation and physicochemical characteristics of anchovy hydrolysates produced by high hydrostatic pressure and enzymatic hydrolysis treatment. Food Engineering Progress 13, 85-91.
  13. Lee BJ. 2009. Sepatation of GABA-producing lactic acid bacteria from tradirional fermented foods and its use for the production of GABA from sea-tangle. MS thesis. Gyeongsang National University, Tongyeong, Korea.
  14. Lee KH, Song BK, Jeong IH, Hong BI, Jung BC and Lee DH. 1997. Processing condition of seasoning material of the mixture of Laminaria and enzyme-treated mackerel meat. Korea J Food SCI Technol 29, 77-81.
  15. Noh YI. 2012. Development and properties of functional seasoning with high yield from byproducts of Alaska pollock and sea tangle. MS thesis. Gyeongsang National University, Tongyeong. Korea.
  16. Oh HS, Kim JS, Kim HS, Jee SJ, Lee JH, Chung IK, Kang KT and Heu MS. 2007. Improvement on the quality and functionality of Skipjack tuna cooking drip using commercial enzymes. J Korea Soc Food Sci Nutr 36, 881-888. https://doi.org/10.3746/jkfn.2007.36.7.881
  17. Park CH, Lee JH, Kang KT, Park JW and Kim JS. 2007. Characterization of acid-soluble collagen from Alaska pollock surimi processing by-products (refiner discharge). Food Sci Biotechnol 16, 549-556. https://doi.org/10.1111/j.1365-2621.1981.tb01849.x
  18. Park YH, Kim SB and Chang DS. 1995. Seafood Processing and Utilization. Hyungsul Publishing Co., Seoul, 147-168.
  19. The Korean Nutrition Society. 2000. Recommended Dietary Allowances for Koreans (7th ed.), Chungang Publishing Co., Seoul, Korea, 43-56, 157-218.
  20. Tsutagawa Y, Hosogai Y and Kawai H. 1994. Comparison of mineral and phosphorus contents of muscle and bone in the wild and cultured horse mackerel. J Food Hyg Soc Japan 34, 315-318.
  21. Ukeda H, Matsuba H, Osajima K, Matsufuji H, Matsui T and Osajima Y. 1992. Peptides from peptic hydrolysate of heated sardine meat that inhibit angiotensin I converting enzyme. Nippon Nogeikagaku Kaishii 66, 25-29. https://doi.org/10.1271/nogeikagaku1924.66.25
  22. Wendel AP. 1999. Recovery and utilization of Pacific whiting frame meat for surimi production. MS thesis. Oregon State University, Corvallis. Oregon. USA.
  23. Wum HC, Chen HM and Shiau CY. 2003. Free amino acids and peptides as related to antioxidant properties in protein hydrolysates of mackerel (Scomber austriasicus). Food Res Int 36, 949-957. https://doi.org/10.1016/S0963-9969(03)00104-2