Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
권호 표지
DOI QR코드

DOI QR Code

Biological Control of White Rot in Garlic Using Burkholderia pyrrocinia CAB08106-4

Burkholderia pyrrocinia CAB08106-4 균주를 이용한 마늘 흑색썩음균핵병의 생물학적 방제

  • Han, Kwang Seop (Chungcheongnam-do Agricultural Research and Extension Services) ;
  • Kim, Buyng Ryun (Chungcheongnam-do Agricultural Research and Extension Services) ;
  • Kim, Jong Tae (Chungcheongnam-do Agricultural Research and Extension Services) ;
  • Hahm, Soo Sang (Chungcheongnam-do Agricultural Research and Extension Services) ;
  • Hong, Ki Heung (Chungcheongnam-do Agricultural Research and Extension Services) ;
  • Chung, Chang Kook (Production Department, Hankook Samgong Co. Ltd.) ;
  • Nam, Yun Gyu (Chungcheongnam-do Agricultural Research and Extension Services) ;
  • Yu, Seung Hun (Department of Applied Biology, Chungnam National University) ;
  • Choi, Jae Eul (Department of Applied Biology, Chungnam National University)
  • Received : 2013.01.25
  • Accepted : 2013.03.20
  • Published : 2013.03.31
Download PDF
⟨ Previous Next ⟩

Abstract

White rot caused by Sclerotium cepivorum was reported to be severe soil-born disease on garlic. Disease progress of white rot of garlic (Allium sativum L.) was investigated during the growing season of 2009 to 2011 at Taean and Seosan areas. The white rot disease on bulb began to occur from late April and peaked in late May. The antifungal bacteria, Burkholderia pyrrocinia CAB08106-4 was tested in field bioassay for suppression of white rot disease. As a result of the nucleotide sequence of the gene 16S rRNA, CAB008106-4 strain used in this study has been identified as B. pyrrocinia. B. pyrrocinia CAB080106-4 isolate suppressed the white rot with 69.6% control efficacy in field test. These results suggested that B. pyrrocinia CAB08106-4 isolate could be an effective biological control agent against white rot of garlic.

Sclerotium cepivorum에 의한 흑색썩음균핵병은 마늘에 큰 피해를 주는 중요한 토양병이다. 충남 서산 및 태안의 마늘 재배농가 포장을 중심으로 2009년부터 3년간 조사한 결과, 흑색썩음균핵병은 4월 하순부터 발생을 시작하고, 5월 중순에 발병이 급격히 증가하여 5월 하순에는 최고 발병률을 나타내었다. 본 시험에 사용된 길항미생물 CAB008106-4 균주는 16S rRNA gene의 염기서열 분석결과 Burkholderia pyrrocinia로 확인되었다. B. pyrrocinia CAB08106-4 균주는 흑색썩음균핵병에 대하여 69.6%의 포장방제효과를 보였다. 이러한 결과로 B. pyrrocinia CAB08106-4는 마늘에 발생하는 흑색썩음균핵병에 대하여 효과적인 생물적 방제제로 활용이 가능함을 확인할 수 있었다.

Keywords

References

  1. Clarkon, J. P., Payne, T., Mead, A. and Whipps, J. M. 2002. Selectin of fungal biological control agents for control of white rot by sclerotial degradation in a UK soil. Plant Pathol. 51: 735-745. https://doi.org/10.1046/j.1365-3059.2002.00787.x
  2. Copping, L. G. 2004. The Manual of Biocontrol Agents. third edition, BCPC. UK. 702 pp.
  3. Cho, W. D. and Kim, W. G. 1996. Qccurrence of white rot on Alliaceous vegetable crops. Korean J. Plant Pathol. 12: 251-254. (In Korean)
  4. Coley-Smith, J. R., Mitchel, C. M. and Sanford, C. E. 1990. Long-term survival of sclerotia of Sclerotium cepivorum and Stromtinia gladioli. Plant Pathol. 39: 58-69. https://doi.org/10.1111/j.1365-3059.1990.tb02476.x
  5. Crowe, F. 1996. White rot. In: Compendium of onion and garlic diseases, ed. by H. F. Schwartz and S. K. Mohaneds. The APS Press, St. Paul, Minnesota, USA.
  6. Han, K. S., Kim, B. R., Hahm, S. S., Yang, E. S., Kwon, K. H. and Choi, J. E. 2011. Biological control of soilborn disease on garlic by antifungal bacteria Burkholderia pyrrocinia. Res. Plant Dis. 17: 423.
  7. Kim, C. H. 2003. Review of disease incidence of major crops in 2002. Res. Plant Dis. 9: 10-17. (In Korean) https://doi.org/10.5423/RPD.2003.9.1.010
  8. Kim, C. H. and Kim, Y. K. 2002. Present status of soilborne disease incidence and scheme for its intergrated management in Korea. Res. Plant Dis. 8: 146-161. (In Korean) https://doi.org/10.5423/RPD.2002.8.3.146
  9. Kim, Y. K., Kwon, M. K., Shim, H. S., Kim, T. S., Yeh, W. H., Cho, W. D., Choi, I. H., Lee, S. C., Ko, S. J., Lee, Y. H. and Lee, C. J. 2005. Various cultural factors associated with disease development of garlic white rot caused by two species of Sclerotium. Res. Plant Dis. 11: 28-34. (In Korean) https://doi.org/10.5423/RPD.2005.11.1.028
  10. Lee, S. Y., Lee, S. B., Kim, Y. K. and Hawang, S. J. 2006. Biological control of white rot accused by Sclerotium cepivorum and Sclerotium sp. using Bacillus subtilis 122 and Trichoderma harzianum 23. Res. Plant Dis. 12: 81-84. (In Korean) https://doi.org/10.5423/RPD.2006.12.2.081
  11. Mclean, K. L. and Stewart, A. 2000. Infection site of Sclerotium cepivorum on onion roots. N. Z. Plant Prot. 53: 118-121.
  12. Ministry for Food, Agriculture, Forestry and Fisheries Republic of Korea. 2008. Food, Agriculture, Forestry and Fisheries Statistical Yearbook.
  13. The Korean Society of Plant Pathology. 2009. List of Plant Diseases in Korea 5: 95-98.

Cited by

  1. Control Activities of Fungicides Against Garlic White Rot Caused by Sclerotium cepivorum vol.19, pp.1, 2015, https://doi.org/10.7585/kjps.2015.19.1.64
  2. Antagonistic Activities of Novel Peptides fromBacillus amyloliquefaciensPT14 againstFusarium solaniandFusarium oxysporum vol.63, pp.48, 2015, https://doi.org/10.1021/acs.jafc.5b04068
  3. Toxicity Evaluation of Burkholderia pyrrocinia CAB08106-4 in Cyprinus carpio and Daphnia magna vol.18, pp.1, 2014, https://doi.org/10.7585/kjps.2014.18.1.21
  4. Antagonistic Activities of Bacillus spp. Strains Isolated from Tidal Flat Sediment Towards Anthracnose Pathogens Colletotrichum acutatum and C. gloeosporioides in South Korea vol.31, pp.2, 2015, https://doi.org/10.5423/PPJ.OA.03.2015.0036
  5. Selection of Antifungal Bacteria Burkholderia lata CAB13001 for Control on Red Pepper Anthracnose and Its Control Efficacy in Field vol.26, pp.4, 2018, https://doi.org/10.11625/KJOA.2018.26.4.649