대한본초학회지 (The Korea Journal of Herbology)

  • 제28권2호
  • /
  • Pages.61-65
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  • 2013
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  • 1229-1765(pISSN)
  • /
  • 2288-7199(eISSN)
대한본초학회 (The Korea Association of Herbology)
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ICR 마우스를 이용한 발효삼출건비탕의 단회투여 독성에 대한 연구

Single Oral Dose Toxicity Test of Fermented Samchulgeonbi-tang Extract in ICR mice

  • 정영필 (한국한의학연구원 한의신약연구그룹) ;
  • 임남희 (한국한의학연구원 한의신약연구그룹) ;
  • 김애영 (한국한의학연구원 한의신약연구그룹) ;
  • 황윤환 (한국한의학연구원 한의신약연구그룹) ;
  • 박화용 (한국한의학연구원 한의신약연구그룹) ;
  • 마진열 (한국한의학연구원 한의신약연구그룹)
  • Jung, Young Pil (KM-Based Herbal Drug Research Group, Korea Institute of Oriental Medicine) ;
  • Yim, Nam-Hui (KM-Based Herbal Drug Research Group, Korea Institute of Oriental Medicine) ;
  • Kim, Aeyung (KM-Based Herbal Drug Research Group, Korea Institute of Oriental Medicine) ;
  • Hwang, Youn-Hwan (KM-Based Herbal Drug Research Group, Korea Institute of Oriental Medicine) ;
  • Park, Hwayong (KM-Based Herbal Drug Research Group, Korea Institute of Oriental Medicine) ;
  • Ma, Jin Yeul (KM-Based Herbal Drug Research Group, Korea Institute of Oriental Medicine)
  • 투고 : 2013.02.12
  • 심사 : 2013.03.22
  • 발행 : 2013.03.30
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초록

Objectives : Samchulgeonbi-tang (shenzhujianpi-tang) has been prescribed as one of traditional herbal medicine for treatment of stomach diseases since ancient time in Korea. Samchulgeonbi-tang extract was fermented by Lactobacillus spp. for improving the effect. However, the toxicity and safety of fermented Samchulgeonbi-tang (FS) extract were not confirmed. Therefore, this study was performed to evaluate the acute toxicity and safety of FS extract. Methods : To evaluate the acute toxicity and safety of FS extract, several doses of FS extract, 0, 500, 1000 and 2000 mg/kg, were orally administered to 20 male and 20 female ICR mice, respectively. After treatment with FS extract, we observed mortality, general toxicity, behavior and change of body weight for the 14 days. After 14 days of oral administration, all mice were sacrificed and hematological parameters were analyzed from blood serum. Results : In present study, the toxic signs such as mortality or abnormal behaviors by FS extract were not observed. There are no significant differences between FS-treated group and control group in body weight, organ weights, and hematological parameters. Conclusions : The remarkable adverse effects by FS extract were not observed in ICR mice. Also, any death was not occurred at all treated FS doses, 500, 1000 and 2000 mg/kg. Therefore, the approximate lethal dose (ALD) of FS extract may be more than 2000 mg/kg.

키워드

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