The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Effects of the ethanol extract of Codonopsis Pilosulae Radix on ovalbumin-induced allergic responses in mice

난알부민 유도 알레르기 면역반응에 대한 당삼(黨參)에탄올추출물의 효능 연구

  • Kang, Seok Yong (Department of Herbology, College of Oriental Medicine, Dongguk University) ;
  • Jung, Jin Ki (Department of Herbology, College of Oriental Medicine, Dongguk University) ;
  • Lee, Sang Kook (College of Pharmacy, Natural Products Research Institute, Seoul National University) ;
  • Lee, Seung Ho (College of Pharmacy, Yeungnam University) ;
  • Park, Yong-Ki (Department of Herbology, College of Oriental Medicine, Dongguk University)
  • 강석용 (동국대학교 한의과대학 본초학교실) ;
  • 정진기 (동국대학교 한의과대학 본초학교실) ;
  • 이상국 (서울대학교 약학대학 천연물과학연구실) ;
  • 이승호 (영남대학교 약학대학 천연물화학교실) ;
  • 박용기 (동국대학교 한의과대학 본초학교실)
  • Received : 2013.02.18
  • Accepted : 2013.03.21
  • Published : 2013.03.30
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Abstract

Objectives : The root of Codonopsis pilosula (Fr.) Nannf. (Codonopsis Pilosulae Radix) has been traditionally used as a oriental medicine with an anti-thrombotic, antidiabetic, anticancer, and anti-gastric ulcer effects and immunological adjuvant. In this study, we investigated the effect of 70% ethanol extract of Codonopsis Pilosulae Radix (CPR-E) on ovalbumin (OVA)-induced allergic responses in mice. Methods : Mice were sensitized (1, 8, and 15 days) with OVA and airway challenged(22, 24, 26, 28, and 30 days) to induced allergic responses. CPR-E extract at doses of 50 and 100 mg/kg/body weight was orally administered from days 21 to 30 consecutively. The levels of allergic mediators such as histamine, OVA-specific immunoglobulin (Ig) E, and Th1/Th2 cytokines such as IFN-${\gamma}$ and IL-4 were measured in the sera of mice by ELISA. The histological change of lung tissue was observed by hematoxylin and eosin (H&E) staining. Results : CPR-E extract significantly decreased the serum levels of histamine, OVA-specific IgE, and IL-4 compared with those of OVA control group, but significantly increased the serum level of IFN-${\gamma}$. Based on H&E staining, CPR-E extract inhibited the infiltration of inflammatory cells into lung tissues with histological changes. Conclusions : These results indicate that CPR-E extract has anti-inflammatory and anti-allergic responses through regulating the cytokine balance, suggesting that the extract may be useful for the treatment of allergic inflammatory diseases such as bronchial asthma and allergic rhinitis.

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References

  1. Jung JK, Park YK. Dose range finding study of KOB03, a new polyherbal medicine for the treatment of allergic rhinitis, by oral administration for four weeks in Sprague-Dawley rats. Kor J Herbology. 2012 ; 27(3) : 101-6. https://doi.org/10.6116/kjh.2012.27.3.106
  2. Park YC, Lim JD, Park YK, Yoon MS, Lee SD. Review : Clinical application and efficacy of herbal medicines by modulating cytokines in atopic dermatitis-induced animal model. Kor J Herbology. 2012 ; 27(4) : 33-44. https://doi.org/10.6116/kjh.2012.27.4.33
  3. Korean Journal of Asthma, Allergy and Clinical Immunology. Asthma and Allergy disease. Seoul : Koonja publisher. 2002 ; 66 : 431-3.
  4. seoul National University College of Medicine. Immunology. Seoul : Seoul National University publisher. 1987 : 188-97.
  5. Ahn KM. Role of mast cell in allergic inflammation and innate immunity. Korean J Pediatrics. 2004 ; 47(11) : 1137-41.
  6. Kim SH, Kim SA, Park MK, Kim SH, Park YD, Na HJ, Kim HM, Shin MK, Ahn KS. Paeonol inhibits anaphylactic reaction by regulation histamine and TNF-${\alpha}$. International Immunopharmacology. 2004 ; 4 : 279-87. https://doi.org/10.1016/j.intimp.2003.12.013
  7. Kawakami T, Galli SJ. Regulation of mast cell and basophil function and survival by IgE. Nature Reviews Immunology. 2002 ; 2 : 773-86. https://doi.org/10.1038/nri914
  8. Choi KJ. Effect of Saururus chinensis (Lour.) Baill Aquacupuncture on Allergy in Mice. Dongeui university. 2004 : 1-23.
  9. Hong WS. JungkyoHuangjenaegyeongsomun. seoul : Dongyang medicine Institute publisher. 1981 : 24, 39, 119.
  10. The whole country a college of Orental medicine The joint textbook publish commission compilatioin. herbology. seoul : yonglimsa. 2006. 578-9.
  11. Ryu HY, Ahn SM, Kim JS, Sohn HY. Evaluation of In-vitro Anticoagulation Activity of 33 Different Medicinal Herbs. Journal of Life Science. 2010 ; 20(6) : 922-8. https://doi.org/10.5352/JLS.2010.20.6.922
  12. Chen HT, Tsai YL, Chen YS, Jong GP, Chen WK, Wang HL, Tsai FJ, Tsai CH, Lai TY, Tzang BS, Huang CY, Lu CY. Dangshen (Codonopsis pilosula) activates IGF-I and FGF-2 pathways to induce proliferation and migration effects in RSC96 Schwann cells. Am J Chin Med. 2010 ; 38(2) : 359-72. https://doi.org/10.1142/S0192415X10007907
  13. He K, Li X, Chen X, Ye X, Huang J, Jin Y, Li P, Deng Y, Jin Q, Shi Q, Shu H. Evaluation of antidiabetic potential of selected traditional Chinese medicines in STZ-induced diabetic mice. J Ethnopharmacol. 2011 ; 137(3) : 1135-42. https://doi.org/10.1016/j.jep.2011.07.033
  14. Sun YX. Immunological adjuvant effect of a water-soluble polysaccharide, CPP, from the roots of Codonopsis pilosula on the immune responses to ovalbumin in mice. Chem Biodivers. 2009 ; 6(6) : 890-6. https://doi.org/10.1002/cbdv.200800154
  15. Wang ZT, Du Q, Xu GJ, Wang RJ, Fu DZ, Ng TB. Investigations on the protective action of Condonopsis pilosula (Dangshen) extract on experimentally-induced gastric ulcer in rats. Gen Pharmacol. 1997 ; 28(3) : 469-73. https://doi.org/10.1016/S0306-3623(96)00047-X
  16. Xu C, Liu Y, Yuan G, Guan M. The contribution of side chains to antitumor activity of a polysaccharide from Codonopsis pilosula. Int J Biol Macromol. 2012 ; 50(4) : 891-4. https://doi.org/10.1016/j.ijbiomac.2012.01.013
  17. Reiss Y, Proudfoot AE, Power CA, Campbell JJ, Butcher EC. CC chemokine receptor (CCR)4 and the CCR10 ligand cutaneou T cell-attracting chemokine (CTACK) in lymphocyte trafficking to inflamed skin. J Exp Med. 2001 ; 194(10) : 1541-7. https://doi.org/10.1084/jem.194.10.1541
  18. Yanase Y, Hiragun T, Uchida K, Ishii K, Oomizu S, Suzuki H, Mihara S, Iwamoto K, Matuo H, Onishi N, Kameyoshi Y, Hide M. Peritoneal injection of fucoidan suppresses the increase of plasma IgE induced by OVA-sensitization. Biochem Biophys Res Commun. 2009 ; 387(3) : 435-9. https://doi.org/10.1016/j.bbrc.2009.07.031
  19. Oh SW, Pae CI, Lee DK, Jones F, Chiang GK, Kim HO, Moon SH, Cao B, Ogbu C, Jeong KW, Kozu G, Nakanishi H, Kahn M, Chi EY, Henderson WR Jr. Tryptase inhibition blocks airway inflammation in a mouse asthma model. J Immunol. 2002 ; 168(4) : 1992-2000. https://doi.org/10.4049/jimmunol.168.4.1992
  20. Sun YX. Immunological adjuvant effect of a water-soluble polysaccharide, CPP, from the roots of Codonopsis pilosula on the immune responses to ovalbumin in mice. Chem Biodivers. 2009 ; 6(6) : 890-6. https://doi.org/10.1002/cbdv.200800154
  21. Kim HJ, Hwang SY, Mok JY, Hwang BS, Jeong SI, Jang SI. Gagam-Gongjin-dan Extract Attenuates Immune Responses to Ovalbumin in Balb/c Mice. Kor J Herbology. 2009；24(4)：127-35.
  22. Yanase Y, Hiragun T, Uchida K, Ishii K, Oomizu S, Suzuki H, Mihara S, Iwamoto K, Matsuo H, Onishi N, Kameyoshi Y, Hide M. Peritoneal injection of fucoidan suppresses the increase of plasma IgE induced by OVA-sensitization. Biochem Biophys Res Commun. 2009 ; 387(3) : 435-9. https://doi.org/10.1016/j.bbrc.2009.07.031
  23. Kimura M, Sasada T, Kanai M, Kawai Y, Yoshida Y, Hayashi E, Iwata S, Takabayashi A. Preventive effect of a traditional herbal medicine, Hochuekki-to, on immunosuppression induced by surgical stress. Surg Today. 2008 ; 38(4) : 316-22. https://doi.org/10.1007/s00595-007-3631-4
  24. Kralovec JA, Power MR, Liu F, Maydanski E, Ewart HS, Watson LV, Barrow CJ, Lin TJ. An aqueous Chlorella extract inhibits IL-5 production by mast cells in vitro and reduces ovalbumininduced eosinophil infiltration in the airway in mice in vivo. Int Immunopharmacol. 2005 ; 5(4) : 689-98. https://doi.org/10.1016/j.intimp.2004.11.016
  25. Weber A, Knop J, Maurer M. Pattern analysis of human cutaneous mast cell populations by total body surface mapping. Br J Dermatol. 2003 ; 148(2) : 224-8. https://doi.org/10.1046/j.1365-2133.2003.05090.x
  26. Schwartz LB, Huff TF. Biology of mast cells. In : Middleton E, editor. Allergy : Principles and practice. 5th ed. Mosby St. Louis. 1998 : 261-76.
  27. Kim YH. The role of mast cells in innate and adaptive immunity. J Life Sci. 2008 ; 18(6) : 891-6. https://doi.org/10.5352/JLS.2008.18.6.891
  28. Jung HY. Molecular bioloy of cytokines. worldscience publishing company. 2002 : 117-24.
  29. Koh YY. Pathophysiology of asthma. Pediatr Allergy Respir Dis. 2000 ; 10(4) : 255-62.