Journal of Ecology and Environment

The Ecological Society of Korea (한국생태학회)
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Evaluation of antimicrobial activity and total phenolic content of three Pinus species

  • Kim, Hyeusoo (Division of Special Purpose Trees, Department of Forest Genetic Resources, Korea Forest Research Institute) ;
  • Lee, Byongsoon (Department of Health Care and Science, College of Alternative Medicine, Jeonju University) ;
  • Yun, Kyeong Won (Department of Oriental Medicine Resources, Sunchon National University)
  • Received : 2013.02.16
  • Accepted : 2013.03.08
  • Published : 2013.03.27
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Abstract

This study compared the antimicrobial activity and total phenolic content of three Pinus plants (Pinus densiflora, P. thunbergii, P. rigida) for the first time. The antimicrobial activity of the water fraction of methanol extract of fresh leaves was stronger than that of fallen leaves at any concentrations. The water fraction of crude methanol extract from fresh leaves of P. thunbergii showed a higher growth inhibitory activity against gram-positive and gram-negative bacteria than that of P. densiflora and P. rigida. The results from the disc diffusion method followed by measurements of minimal inhibition concentration (MIC) indicate that Bacillus subtilis was the most sensitive microorganism with the lowest MIC value. The highest total phenolic content was found in fresh leaves of P. rigida and P. thunbergii. The assay showed that the fresh leaves of the three Pinus plants contained higher total phenolic content than fallen leaves of the three plants. The antimicrobial activity was related with the total phenolic content.

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References

  1. Al-Zoreky NS. 2009. Antimicrobial activity of pomegranate (Punica granatum L.) fruit peels. Int J Food Microbiol 134: 244-248. https://doi.org/10.1016/j.ijfoodmicro.2009.07.002
  2. Baydar NG, Ozkan G, Sagdic O. 2004. Total phenolic contents and antimicrobial activities of grape (Vitis vinifera L.) extracts. Food Control 15: 335-339. https://doi.org/10.1016/S0956-7135(03)00083-5
  3. Bonanomi G, Vinale F, Scala F. 2009. The role of natural products in plant-microbe interations. In: Plant-derived Natural Products (Osbourn AE, Lanzotti V, eds), Springer, Dordrecht Heidelberg London New York, pp 301-320.
  4. Boudet AM. 2007. Evalution and current status of research in phenolic compounds. Phytochemistry 68: 2722-2735. https://doi.org/10.1016/j.phytochem.2007.06.012
  5. Boussaada O, Chriaa J, Nabil R, Ammar S, Saidana D, Mahjoub MA, Chraeif I, Helal AN, Mighri Z. 2008. Antimicrobial and antioxidant activities of methanol extracts of Evax pygmea (Asteraceae) growing wild in Tunisia. World J Microbiol Biotech 24: 1289-1296. https://doi.org/10.1007/s11274-007-9600-7
  6. Duffy CF, Power RF. 2001. Antioxidant and antimicrobial properties of some Chinese plant extracts. Int J Antimicrobial Agents 17: 527-529. https://doi.org/10.1016/S0924-8579(01)00326-0
  7. Fattouch S, Carboni P, Corone V, Tuberoso CIG, Angioni A, Dessel S. 2007. Antimicrobial activity of Tunisian quince (Cydonia oblonga Miller) pulp and peel polyphenolic extracts. J Agric Food Chem 55: 963-969. https://doi.org/10.1021/jf062614e
  8. Hagerman AE, Riedl KM, Jones GA, Sovik KN, Ritchard NT, Hartzfeldt PW. 1998. High molecular weight plant poly-phenolics (tannins) as biological antioxidants. J Agric Food Chem 46: 1887-1892. https://doi.org/10.1021/jf970975b
  9. Hwang YH, Lee HS. 2002. Antibacterial activity of Pinus densiflora leaf-derived components toward human intestinal bacteria. J Microbiol Biotechnol 12: 610-616.
  10. Hwang BH, Cho JH, Ham SS, Kang HY. 2000. Chemical analysis of pine leaves. J Kor Soc Food Sci Nutr 29: 6-9.
  11. Im RJ. 1998. Flora Medica Coreana, Vol. 1. Part Modern Medicine. Agricultural Pub. House, Pyongyang, North Korea.
  12. Jang MJ, Kim YH, An BJ, Lee CE, Lee JT, Kim SH, Lee BG, Lee DH. 2008. Study on anti-inflammatory and anti-micorbial effect of Pinus rigida Mill. inner bark extracts as a cosmetic material. J Kor For Soc 97: 215-220.
  13. Jeon HJ, Lee KS, Ahn YJ. 2001. Growth-inhibiting effects of constituents of Pinus densiflora leaves on human intestinal bacteria. Food Sci Biotechnol 10: 403-407.
  14. Kim H, Song MJ, Potter D. 2006. Medicinal efficacy of plants utilized as temple food in traditional Korean Buddhism. J Ethnopharmacol 104: 32-46. https://doi.org/10.1016/j.jep.2005.08.041
  15. Kim YO, Lee HJ. 1996. Identification and effects of phenolic compounds from some plant. Kor J Ecol 19: 329-340.
  16. Kim YW, Moon HK. 2007. Regeneration of plant by somatic embryogenesis in Pinus rigida ${\times}$ P. taeda. In Vitro Cell. Dev Biol Plant 43: 335-342. https://doi.org/10.1007/s11627-007-9045-6
  17. Ku CS, Jang JP, Mun SP. 2007. Exploitation of polyphenolrich pine barks for potent antioxidant activity. J Wood Sci 53: 524-528. https://doi.org/10.1007/s10086-007-0896-6
  18. Kujumgiefv A, Bankova V, Ignatova A, Popov S. 1993. Antibacterial activity of propolis, some of its components and their analogs. Pharmazie 48: 785-786.
  19. Kultur S. 2007. Medicinal plants used in Kirklareli Province (Turkey). J Ethnopharmacol 111: 341-364. https://doi.org/10.1016/j.jep.2006.11.035
  20. Kumar VP, Chauhan NS, Padh H, Rajani M. 2006. Search for antibacterial and antifungal agents from selected Indian medicinal plants. J Ethnopharmacol 107: 182-188. https://doi.org/10.1016/j.jep.2006.03.013
  21. Lee SK, Lee HJ, Min HY, Park EJ, Lee KM, Ahn YH, Cho YJ, Pyee JH. 2005. Antibacterial and antifungal activity of pinosylvin, a constituent of pine. Fitoterapia 76: 258-260. https://doi.org/10.1016/j.fitote.2004.12.004
  22. Lim YS, Park KN, Bae MJ, Lee SH. 2001. Antimicrobial effects of ethanol extracts of Pinus densiflora Sieb. and Zucc. on lactic acid bacteria. J Kor Soc Food Sci Nutr 30: 1158-1163.
  23. Lizcano LJ, Bakkali F, Ruiz-Larrea BR, Ruiz-Sanz JI. 2010. Antioxidant activity and polyphenol content of aqueous extracts from Colombian Amazonian plants with medicinal use. Food Chem 119: 1566-1570. https://doi.org/10.1016/j.foodchem.2009.09.043
  24. Mahasneh AM. 2002. Screening of some indigenous Qatari medicinal plants for antimicrobial activity. Phytother Res 16: 751-753. https://doi.org/10.1002/ptr.1037
  25. Magasneh AM, El-Oqlah AA. 1999. Antimicrobial activity of extracts from herbal species used in the traditional medicine of Jordan. J Ethnopharmacol 64: 271-276. https://doi.org/10.1016/S0378-8741(98)00132-9
  26. Ogawa M. 1979. Microbial flora in Pinus thunbergii forest of coastal sand dune. Bull For Prod Res Inst 307: 107-124.
  27. Padda MS, Picha DH. 2008. Effect of low temperature storage on phenolic composition and antioxidant activity of sweet potatoes. Postharvest Biol Tech 47: 176-180. https://doi.org/10.1016/j.postharvbio.2007.06.014
  28. Ribeiro SMR, Barbosa LCA, Queiroz JH, Knodler M, Schieber A. 2008. Phenolic compounds and antioxidant capacity of Brazilian mango (Mangifera indica L.) varieties. Food Chem 110: 620-626. https://doi.org/10.1016/j.foodchem.2008.02.067
  29. Rim YS, Park YM, Park MS, Kim JY, Kim MJ, Choi YH. 2000. Screening of antioxidants and antimicrobial activity in native plants. Kor J Med Crop Sci 8: 342-350.
  30. Rodriguez Vaquero MJ, Tomassini Serravalle LR, Manca De Nadra MC, Strasser De Saad AM. 2010. Antioxidant capacity and antibacterial activity of phenolic compounds from Argentinean herbs infusions. Food Control 21: 779-785. https://doi.org/10.1016/j.foodcont.2009.10.017
  31. Satake Y, Hara H, Watari S, Tominari T. 1989. Wild flowers of Japan: Woody plants. Heibonsha Ltd., Tokyo.
  32. Smith-Palmer A, Stewart J, Fyfe L. 1998. Antimicrobial properties of plant essential oils and essences against five important food-born pathogens. Lett Microbiol 26: 118-122. https://doi.org/10.1046/j.1472-765X.1998.00303.x
  33. Soong YY, Barlow PJ. 2004. Antioxidant activity and phenolic content of selected fruit seeds. Food Chem 88: 411-417. https://doi.org/10.1016/j.foodchem.2004.02.003
  34. Su XY, Wang ZY, Liu JR. 2009. In vitro and in vivo antioxidant activity of Pinus koraiensis seed extract containing phenolic compounds. Food Chem 117: 681-686. https://doi.org/10.1016/j.foodchem.2009.04.076
  35. Watanabe K, Momose F, Handa H. 1995. Interaction between influenza virus pine cone antitumor substance that inhibit the virus multiplication. Biochem Biophysic Res Comm 214: 318-323. https://doi.org/10.1006/bbrc.1995.2290
  36. Weerakkody NS, Caffin N, Tumer MS, Dykes GA. 2010. In vitro antimicrobial activity of less-utilized spice and herb extracts against selected food-borne bacteria. Food Control 21: 1408-1414. https://doi.org/10.1016/j.foodcont.2010.04.014
  37. Wojdylo A, Oszmianski J, Czemerys R. 2007. Antioxidant activity and phenolic compounds in 32 selected herbs. Food Chem 105: 940-949. https://doi.org/10.1016/j.foodchem.2007.04.038
  38. Xia D, Wu X, Shi J, Yang Q, Zhang Y. 2010. Phenolic compounds from the edible seeds extract of Chinese Mei (Prunus mume Sieb. et Zucc) and their antimicrobial activity. LWT-Food Sci Tech 44: 347-349.
  39. Yang MS, Ha YL, Nam SH, Choi SU, Jang DS. 1995. Screening of domestic plants with antibacterial activity. Agric Chem Biotech 38: 584-589.
  40. Yossa N, Patel J, Miller P, Lo YM. 2010. Antimicrobial activity of essential oils against Escherichia coli O157: H7 in organic soil. Food Control 21: 1458-1485. https://doi.org/10.1016/j.foodcont.2010.03.016
  41. Yun KW, Jeong HJ, Kim JH. 2008. The influence of growth season on the antimicrobial and antioxidative activity in Artemisia princeps var. orientalis. Ind Crops Prod 27: 69-74. https://doi.org/10.1016/j.indcrop.2007.07.017

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