Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Anti-Inflammatory Effects of Extracts from Caesalpinia sappan L. on Skin Inflammation

TPA로 유도된 마우스 귀 부종 동물모델에서 소목추출물의 항염증 효과

  • Eum, Won Sik (Dept. of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University) ;
  • Lee, Kwang-Jae (Gangwon Agricultural Research and Extention Services) ;
  • Kim, Dae Won (Dept. of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University) ;
  • Lim, Soon Sung (Dept. of Food Sciences and Nutrition and RIC Center, Hallym University) ;
  • Kang, Il-Jun (Dept. of Food Sciences and Nutrition and RIC Center, Hallym University) ;
  • Park, Jinseu (Dept. of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University) ;
  • Choi, Soo Young (Dept. of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University)
  • 음원식 (한림대학교 바이오메디컬학과 및 생명공학연구소) ;
  • 이광재 (강원도농업기술원) ;
  • 김대원 (한림대학교 바이오메디컬학과 및 생명공학연구소) ;
  • 임순성 (한림대학교 식품영양학과 및 RIC 센터) ;
  • 강일준 (한림대학교 식품영양학과 및 RIC 센터) ;
  • 박진서 (한림대학교 바이오메디컬학과 및 생명공학연구소) ;
  • 최수영 (한림대학교 바이오메디컬학과 및 생명공학연구소)
  • Received : 2012.11.12
  • Accepted : 2012.12.14
  • Published : 2013.03.31
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Abstract

This study investigated the anti-inflammatory effects of extracts from Caesalpinia sappan L. (CSL) on 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced ear edema in mice. Skin inflammation was detected by immunohistochemistry and the protein and mRNA expression levels of cyclooxygenase-2 (COX-2) and cytokines (IL-6, IL-$1{\beta}$ and TNF-${\alpha}$) detected by Western blotting and RT-PCR. The activation of nuclear factor-kappa B (NF-${\kappa}B$) and mitogen-activated protein kinase (MAPK) were analyzed by Western blotting. CSL extracts markedly inhibited the TPA-induced expression of COX-2 and pro-inflammatory cytokines. Also, CSL extracts significantly reduced the activation of NF-${\kappa}B$ and MAPK. These results suggest that CSL extracts may serve as therapeutic agents against skin diseases related to inflammation.

본 연구를 통하여 TPA로 유도한 마우스 귀 부종 염증반응에 대한 소목추출물의 항염증 효능과 기전을 확인하였다. 소목추출물은 TPA로 유도한 마우스 귀 부종을 억제하였으며, TPA에 의한 염증관련 단백질인 COX-2 발현 및 cytokine(IL-6, TNF-${\alpha}$ 그리고 IL-$1{\beta}$)의 mRNA 발현을 현저히 감소시켰다. 또한 TPA에 의한 NF-${\kappa}B$ 및 MAPK의 활성을 억제하였다. 본 연구 결과, 소목추출물은 NF-${\kappa}B$ 및 MAPK의 신호전달을 억제함으로서 항염증 효능을 나타내었다.

Keywords

References

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