Parasites, Hosts and Diseases

  • 제51권6호
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  • Pages.763-766
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  • 2013
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  • 2982-5164(pISSN)
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  • 1738-0006(eISSN)
대한기생충학·열대의학회 (The Korea Society for Parasitology and Tropical Medicine)
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Evaluation of IgG4 Subclass Antibody Detection by Peptide-Based ELISA for the Diagnosis of Human Paragonimiasis Heterotrema

  • Intapan, Pewpan M. (Department of Parasitology and Research and Diagnostic Center for Emerging Infectious Diseases, Faculty of Medicine, Khon Kaen University) ;
  • Sanpool, Oranuch (Department of Parasitology and Research and Diagnostic Center for Emerging Infectious Diseases, Faculty of Medicine, Khon Kaen University) ;
  • Janwan, Penchom (Department of Parasitology and Research and Diagnostic Center for Emerging Infectious Diseases, Faculty of Medicine, Khon Kaen University) ;
  • Laummaunwai, Porntip (Department of Parasitology and Research and Diagnostic Center for Emerging Infectious Diseases, Faculty of Medicine, Khon Kaen University) ;
  • Morakote, Nimit (Department of Parasitology, Faculty of Medicine, Chiang Mai University) ;
  • Kong, Yoon (Department of Molecular Parasitology, Sungkyunkwan University School of Medicine and Center for Molecular Medicine, Samsung Biomedical Research Institute) ;
  • Maleewong, Wanchai (Department of Parasitology and Research and Diagnostic Center for Emerging Infectious Diseases, Faculty of Medicine, Khon Kaen University)
  • 투고 : 2013.05.30
  • 심사 : 2013.10.11
  • 발행 : 2013.12.31
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초록

A synthetic peptide was prepared based on the antigenic region of Paragonimus westermani pre-procathepsin L, and its applicability for immunodiagnosis for human paragonimiasis (due to Paragonimus heterotremus) was tested using an ELISA to detect IgG4 antibodies in the sera of patients. Sera from other helminthiases, tuberculosis, and healthy volunteers were used as the references. This peptide-based assay system gave sensitivity, specificity, accuracy, and positive and negative predictive values of 100%, 94.6%, 96.2%, 100%, and 88.9%, respectively. Cross reactivity was frequently seen against the sera of fascioliasis (75%) and hookworm infections (50%). Since differential diagnosis between paragonimiasis and fascioliasis can be easily done by clinical presentation and fascioliasis serology, this cross reaction is not a serious problem. Sera from patients with other parasitoses (0-25%) rarely responded to this synthetic antigen. This synthetic peptide antigen seems to be useful for development of a standardized diagnostic system for paragonimiasis.

키워드

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피인용 문헌

  1. North American paragonimiasis: epidemiology and diagnostic strategies vol.13, pp.6, 2015, https://doi.org/10.1586/14787210.2015.1031745
  2. A tool for mass-screening of paragonimiasis: an enzyme-linked immunosorbent assay with urine samples vol.44, pp.1, 2013, https://doi.org/10.1186/s41182-016-0019-4
  3. 만성 복통과 발진을 주소로 내원한 6세 여아에서 진단된 폐흡충증 1예 vol.25, pp.1, 2018, https://doi.org/10.14776/piv.2018.25.1.54