Parasites, Hosts and Diseases

The Korea Society for Parasitology and Tropical Medicine (대한기생충학·열대의학회)
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Serological and Molecular Characteristics of the First Korean Case of Echinococcus multilocularis

  • Jeong, Jin-Sook (Department of Pathology, General Surgery, Dong-A University College of Medicine) ;
  • Han, Sang-Young (Department of Internal Medicine, General Surgery, Dong-A University College of Medicine) ;
  • Kim, Young-Hoon (Department of General Surgery, Dong-A University College of Medicine) ;
  • Sako, Yasuhito (Department of Parasitology, Asahikawa Medical University) ;
  • Yanagida, Tetsuya (Department of Parasitology, Asahikawa Medical University) ;
  • Ito, Akira (Department of Parasitology, Asahikawa Medical University) ;
  • Chai, Jong-Yil (Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine)
  • Received : 2013.08.23
  • Accepted : 2013.09.05
  • Published : 2013.10.31
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Abstract

In December 2011, we reported an autochthonous case of Echinococcus multilocularis infection in a 42-yearold woman in Korea. The diagnosis was based on histopathological findings of the surgically resected liver cyst. In the present study, we evaluated the serological and molecular characteristics of this Korean E. multilocularis case. The patient's serum strongly reacted with affinity-purified native Em18 and recombinant Em18 antigens (specific for E. multilocularis) but negative for recombinant antigen B8/1 (reactive for Echinococcus granulosus). In immunoaffinity chromatography, the serum also strongly reacted with E. multilocularis and only weakly positive for E. granulosus. We determined the whole nucleotide sequence of cox1 (1,608 bp) using the paraffin-embedded cystic tissue which was compared with E. multilocularis isolates from China, Japan, Kazakhstan, Austria, France, and Slovakia. The Korean case showed 99.8-99.9% similarity with isolates from Asia (the highest similarity with an isolate from Sichuan, China), whereas the similarity with European isolates ranged from 99.5 to 99.6%.

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References

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