Parasites, Hosts and Diseases

  • 제51권5호
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  • Pages.573-577
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  • 2013
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  • 2982-5164(pISSN)
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  • 1738-0006(eISSN)
대한기생충학·열대의학회 (The Korea Society for Parasitology and Tropical Medicine)
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Detection of Acute Toxoplasmosis in Pigs Using Loop-Mediated Isothermal Amplification and Quantitative PCR

  • Wang, Yanhua (State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences) ;
  • Wang, Guangxiang (State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences) ;
  • Zhang, Delin (Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences) ;
  • Yin, Hong (Key Laboratory of Veterinary Public Health of the Ministry of Agriculture, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences) ;
  • Wang, Meng (State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences)
  • 투고 : 2013.04.28
  • 심사 : 2013.07.14
  • 발행 : 2013.10.31
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초록

A loop-mediated isothermal amplification (LAMP) assay allows rapid diagnosis of Toxoplasma gondii infection. In the present study, the LAMP assay was evaluated using blood from both naturally and experimentally infected pigs. The sensitivity of the LAMP assay was compared with that of Q-PCR. Both assays detected T. gondii in the blood of experimentally infected pigs, with 100% agreement. In infected blood samples, the parasite was detected as early as 2 days post-infection and reached a peak in 3-5 days. In 216 field serum samples, the detection rates of LAMP and Q-PCR assays were 6.9% and 7.8%, respectively. This result indicates that the sensitivity of the LAMP assay was slightly lower than that of the Q-PCR assay. However, the LAMP may be an attractive diagnostic method in conditions where sophisticated and expensive equipment is unavailable. This assay could be a powerful supplement to current diagnostic methods.

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참고문헌

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피인용 문헌

  1. Diagnosis of toxoplasmosis and typing of Toxoplasma gondii vol.8, pp.1, 2013, https://doi.org/10.1186/s13071-015-0902-6
  2. Molecular detection and phylogenetic analyses of Toxoplasma gondii from naturally infected sheep in Northern and Central Tunisia vol.3, pp.1, 2013, https://doi.org/10.1002/vms3.53
  3. RAA-Cas12a-Tg: A Nucleic Acid Detection System for Toxoplasma gondii Based on CRISPR-Cas12a Combined with Recombinase-Aided Amplification (RAA) vol.9, pp.8, 2013, https://doi.org/10.3390/microorganisms9081644