Parasites, Hosts and Diseases

The Korea Society for Parasitology and Tropical Medicine (대한기생충학·열대의학회)
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A Rapid Diagnostic Test for Toxoplasmosis using Recombinant Antigenic N-terminal Half of SAG1 Linked with Intrinsically Unstructured Domain of GRA2 Protein

  • Song, Kyoung Ju (Department of Parasitology, Catholic Institute of Parasitic Disease, College of Medicine, Catholic University of Korea) ;
  • Yang, Zhaoshou (Department of Parasitology, Catholic Institute of Parasitic Disease, College of Medicine, Catholic University of Korea) ;
  • Chong, Chom-Kyu (Asan Biotech Institute, Asan Pharm Co Ltd.) ;
  • Kim, Jin-Soo (Asan Biotech Institute, Asan Pharm Co Ltd.) ;
  • Lee, Kyung Chan (Asan Biotech Institute, Asan Pharm Co Ltd.) ;
  • Kim, Tong-Soo (Department of Parasitology, Inha University School of Medicine) ;
  • Nam, Ho-Woo (Department of Parasitology, Catholic Institute of Parasitic Disease, College of Medicine, Catholic University of Korea)
  • Received : 2013.07.08
  • Accepted : 2013.09.02
  • Published : 2013.10.31
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Abstract

Toxoplasma gondii is an apicomplexan parasite with a broad host range of most warm-blooded mammals including humans, of which one-thirds of the human population has been infected worldwide which can cause congenital defects, abortion, and neonatal complications. Here, we developed a rapid diagnostic test (RDT) for T. gondii infection. Antigenic N-terminal half of the major surface antigen (SAG1) was linked with intrinsically unstructured domain (IUD) of dense granule protein 2 (GRA2). The recombinant GST-GRA2-SAG1A protein was successfully expressed and purified as 51 kDa of molecular weight. Furthermore, antigenicity and solubility of the rGST-GRA2-SAG1A protein were significantly increased. The overall specificity and sensitivity of GST-GRA2-SAG1A loaded RDT (TgRDT) were estimated as 100% and 97.1% by comparing with ELISA result which uses T. gondii whole cell lysates as the antigen. The TgRDT tested with Uganda people sera for field trial and showed 31.9% of seroprevalence against T. gondii antibody. The TgRDT is proved to be a kit for rapid and easy to use with high accuracy, which would be a suitable serodiagnostic tool for toxoplasmosis.

Keywords

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