Parasites, Hosts and Diseases

The Korea Society for Parasitology and Tropical Medicine (대한기생충학·열대의학회)
권호 표지
DOI QR코드

DOI QR Code

Development of a Disperse Dye Immunoassay Technique for Detection of Antibodies against Neospora caninum in Cattle

  • Received : 2012.04.19
  • Accepted : 2012.10.04
  • Published : 2013.03.15
Download PDF
⟨ Previous Next ⟩

Abstract

In this study a disperse dye immunoassay method was standardized and evaluated for detection of antibodies against Neospora caninum in cattle. Sera from 150 cattle with a recent history of abortion were collected and tested by commercial ELISA kit and a standardized in-house dye immunoassay system. The positivity rate for the sera used in this study was 34.6% for the disperse dye immunoassay (DDIA) compared to 32% obtained by ELISA kit. This study showed no significant difference between DDIA and ELISA. The results indicated that the DDIA provide an economic, simple, rapid and robust test for detection of N. caninum infection in cattle.

Keywords

References

  1. Reiterova K, Pilovska S, Antolova D, Dubinsk P. Neospora caninum, potential cause of abortions in dairy cows: the current serological follow-up in Slovakia. Vet Parasitol 2009; 159: 1-6. https://doi.org/10.1016/j.vetpar.2008.10.008
  2. Dubey JP, Vianna MCB, Kwok OCH, Hill DE, Miska KB, Tuo W, Velmurugan GV, Conors M, Jenkins MC. Neosporosis in Beagle dogs: Clinical signs, diagnosis, treatment, isolation and genetic characterization of Neospora caninum. Vet Parasitol 2007; 149: 158-166. https://doi.org/10.1016/j.vetpar.2007.08.013
  3. Bjerkas I, Mohn SF, Presthus J. Unidentified cyst-forming-sporozoon causing encephalomyelitis and myositis in dogs. Z Parasitenkd 1984; 70: 271-274. https://doi.org/10.1007/BF00942230
  4. Dubey J, Schares G. Diagnosis of bovine neosporosis. Vet Parasitol 2006; 140: 1-34. https://doi.org/10.1016/j.vetpar.2006.03.035
  5. Bjorkman C, Uggla A. Serological diagnosis of Neospora caninum infection. Int J Parasitol 1999; 29: 1497-1507. https://doi.org/10.1016/S0020-7519(99)00115-0
  6. Packham A, Sverlow K, Conrad PA, Loomis EF, Rowe JD, Anderson ML, Marsh AE, Cray C, Barr BC. A modified agglutination test for Neospora caninum: Development, optimization, and comparison to the indirect fluorescent-antibody test and enzyme-linked immunosorbent assay. Clin Diagnos Lab Immunol 1998; 5: 467-473.
  7. Baszler T, Knowles D, Dubey J, Gay J, Mathison B, Mcelwain T. Serological diagnosis of bovine neosporosis by Neospora caninum monoclonal antibody-based competitive inhibition enzyme-linked immunosorbent assay. J Clin Microbiol 1996; 34: 1423-1428.
  8. Fisa R, Gallego M, Riera C, Aisa MJ, Valls D, Serra T, de Colmenares M, Castillejo S, Portus M. Serologic diagnosis of canine leishmaniasis by dot-ELISA. J Vet Diagno Invest 1997; 9: 50-55. https://doi.org/10.1177/104063879700900109
  9. Baszler T, Adams S, Vander-Schalie J, Mathison B, Kostovic M. Validation of a commercially available monoclonal antibody-based competitive-inhibition enzyme-linked immunosorbent assay for detection of serum antibodies to Neospora caninum in cattle. J Clin Microbiol 2001; 39: 3851-3857. https://doi.org/10.1128/JCM.39.11.3851-3857.2001
  10. Xiang X, Tianping W, Zhigang T. Development of a rapid, sensitive, dye immunoassay for schistosomiasis diagnosis: a colloidal dye immunofiltration assay. J Immunol Methods 2003; 280: 49- 57. https://doi.org/10.1016/S0022-1759(03)00196-0
  11. Mistrello G, Gentili M, Falagiani P, Roncarolo D, Riva G, Tinelli M. Dot immunobinding assay as a new diagnostic test for human hydatid disease. Immunol Letters 1995; 47: 79-85. https://doi.org/10.1016/0165-2478(95)00068-G
  12. Rabello AL, DiasNeto E, Garsia MM, Katz N. Dot-dye immunoassay for the diagnosis of schistosomiasis mansoni. Memo Inst Oswaldo Cruz 1992; 87: 187-190.
  13. Snowden K, Hommel M. Antigen detection immunoassay using dipsticks and colloidal dyes. J Immunol Methods 1991; 140: 57-65. https://doi.org/10.1016/0022-1759(91)90126-Z
  14. Bjorkman C, Lunden A, Holmdahl J, Barber J, Trees AJ, Uggla A. Neospora caninurn in dogs: detection of antibodies by ELISA using an iscom antigen. Parasite Immunol 1994; 16: 643-648. https://doi.org/10.1111/j.1365-3024.1994.tb00320.x
  15. Trees AJ, Guy F, Tennant BJ, Balfour AH, Dubey JP. Prevalence of antibodies to Neospora caninum in a population of urban dogs in England. Vet Rec 1993; 6: 125-126.
  16. Gribnau T, Van Sommeren A, Van Dinther F. DIA disperse immunoassay. In Chaiken M. et al., eds, Affinity Chromatography and Biological Recognition: Orlando. Academic Press. 1983. p 375-380.
  17. Enoe C, Georgiadis MP, Johnson WO. Estimation of sensitivity and specificity of diagnostic tests and disease prevalence when the true disease state is unknown. Prevent Vet Med 2000; 45: 61-81. https://doi.org/10.1016/S0167-5877(00)00117-3
  18. Jing L, Jinshu Y, Ming W, Qun L, Wei Z, Chong D, Jun D. Serodiagnosis of Neospora caninum infection in cattle using a recombinant tNcSRS2 protein-based ELISA. Vet Parasitol 2007; 143: 358-363. https://doi.org/10.1016/j.vetpar.2006.08.034
  19. Bjorkman C, Holmdahl OJ, Uggla A. An indirect enzyme-linked immunoassay (ELISA) for demonstration of antibodies to Neospora caninum in serum and milk of cattle. Vet Parasitol 1997; 68: 251-260. https://doi.org/10.1016/S0304-4017(96)01076-X
  20. Wan Omar A, Low CK, Vivian JG, Sulaiman O, Yusof S. A colorimetric dipstick colloidal dye immunoassay for rapid diagnosis of active cases of canine dirofilariasis. Asian Pacific J Molecul Biol Biotech 1998; 6: 41-46.
  21. Jin S, Chang ZY, Ming X, Li MC, Wei H, You SL, Xiao HG. Fast dipstick dye immunoassay for detection of immunoglobulin G (IgG) and IgM antibodies of human toxoplasmosis. Clin diagn lab immunol 2005; 12: 198-201.
  22. Gasem MH, Smits HL, Goris MGA, Dolman MV. Evaluation of a simple and rapid dipstick assay for the diagnosis of typhoid fever in Indonesia. J Med Microbial 2002; 51: 173-177.
  23. Kashiwazaki Y, Kanitpun R, Suteeraparp P, Boonchit S. A preliminary comparative study of a dipstick colloidal dye immunoassay and two antigen-detection ELISAs for diagnosis of Trypanosoma evansi infection in cattle. Vet Res Communicat 2000; 24: 533-544. https://doi.org/10.1023/A:1006439902072
  24. Wan Omar A, Sulaiman O, Yusof S, Ismail G, Fatmah MS, Rahmah N, Khairul AA. Epidemiological screening of lymphatic filariasis among immigrants using dipstick colloidal dye immunoassay. Malays J Med Sci 2001; 8: 19-24.
  25. Smits HL, Basahi MA, Diaz R, Marrodan T, Douglas JT, Rocha A, Veerman J, Zheludkov MM, Witte OW, de Jong J, Gussenhoven GC, Goris MG, van Der Hoorn MA. Development and Evaluation of a Rapid Dipstick Assay for Serodiagnosis of Acute Human Brucellosis. J Clin Microbial 1999; 9: 4179-4182.
  26. Schallig HDFH, Cardoso L, Hommers M, Kroon N, Belling G, Rodrigues M, Semiao-Santos SJ, Vetter H. Development of a dipstick assay for detection of leishmania-specific canine antibodies. J Clin Microbial 2004; 3: 193-197.
  27. Zhu Y, He W, Liang Y, Xu M, Yu C, Hua W, Chao G. Development of a rapid, simple dipstick dye immunoassay for schistosomiasis diagnosis. J Immunol Methods 2000; 266: 1-5.
  28. Wang SJ, Chang WF, Wang MY, Hsiung KP, Liu YC. Development of a disperse dye immunochromatographic test for the detection of antibodies against infectious bursal disease virus. Vet Immunol Immunopathol 2008; 125: 284-290. https://doi.org/10.1016/j.vetimm.2008.05.022