Parasites, Hosts and Diseases

The Korea Society for Parasitology and Tropical Medicine (대한기생충학·열대의학회)
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Kinetics of IL-23 and IL-12 Secretion in Response to Toxoplasma gondii Antigens from THP-1 Monocytic Cells

  • Quan, Juan-Hua (Department of Infection Biology and Research Institute for Medical Sciences, Chungnam National University School of Medicine) ;
  • Zhou, Wei (Department of Infection Biology and Research Institute for Medical Sciences, Chungnam National University School of Medicine) ;
  • Cha, Guang-Ho (Department of Infection Biology and Research Institute for Medical Sciences, Chungnam National University School of Medicine) ;
  • Choi, In-Wook (Department of Infection Biology and Research Institute for Medical Sciences, Chungnam National University School of Medicine) ;
  • Shin, Dae-Whan (Department of Infection Biology and Research Institute for Medical Sciences, Chungnam National University School of Medicine) ;
  • Lee, Young-Ha (Department of Infection Biology and Research Institute for Medical Sciences, Chungnam National University School of Medicine)
  • Received : 2012.11.23
  • Accepted : 2013.01.15
  • Published : 2013.03.15
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Abstract

IL-23 and IL-12 are structurally similar and critical for the generation of efficient cellular immune responses. Toxoplasma gondii induces a strong cell-mediated immune response. However, little is known about IL-23 secretion profiles in T. gondii-infected immune cells in connection with IL-12. We compared the patterns of IL-23 and IL-12 production by THP-1 human monocytic cells in response to stimulation with live or heat-killed T. gondii tachyzoites, or with equivalent quantities of either T. gondii excretory/secretory proteins (ESP) or soluble tachyzoite antigen (STAg). IL-23 and IL-12 were significantly increased from 6 hr after stimulation with T. gondii antigens, and their secretions were increased with parasite dose-dependent manner. IL-23 concentrations were significantly higher than those of IL-12 at the same multiplicity of infection. IL-23 secretion induced by live parasites was significantly higher than that by heat-killed parasites, ESP, or STAg, whereas IL-12 secretion by live parasite was similar to those of ESP or STAg. However, the lowest levels of both cytokines were at stimulation with heat-killed parasites. These data indicate that IL-23 secretion patterns by stimulation with various kinds of T. gondii antigens at THP-1 monocytic cells are similar to those of IL-12, even though the levels of IL-23 induction were significantly higher than those of IL-12. The detailed kinetics induced by each T. gondii antigen were different from each other.

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