Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Activity-guided Screening of Anti-inflammatory Compounds from the Hexane Extracts of Schisandra chinensis Fruit

생리활성분획 추적방법을 통한 오미자 추출물의 항염증 활성 분석

  • Choi, Hee Jung (Department of Anatomy, School of Medicine, Pusan National University) ;
  • Choi, Young-Whan (Department of Horticultural Bioscience, College of Natural Resources and Life Science, Pusan National University) ;
  • Baek, Sun-Yong (Department of Anatomy, School of Medicine, Pusan National University) ;
  • Kim, Bong-Seon (Department of Anatomy, School of Medicine, Pusan National University) ;
  • Ahn, Soon Cheol (Department of Microbiology and Immunology, School of Medicine, Pusan National University) ;
  • Rhee, Moon-Soo (Korea Research Institute of Bioscience & Biotechnology) ;
  • Yoon, Sik (Department of Anatomy, School of Medicine, Pusan National University)
  • 최희정 (부산대학교 의학전문대학원 해부학교실) ;
  • 최영환 (부산대학교 생명자원과학대학 원예생명과학과) ;
  • 백선용 (부산대학교 의학전문대학원 해부학교실) ;
  • 김봉선 (부산대학교 의학전문대학원 해부학교실) ;
  • 안순철 (부산대학교 의학전문대학원 미생물학 및 면역학교실) ;
  • 이문수 (한국생명공학연구원 생명자원센터) ;
  • 윤식 (부산대학교 의학전문대학원 해부학교실)
  • Received : 2012.01.04
  • Accepted : 2012.02.22
  • Published : 2013.02.28
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Abstract

Schisandra chinensis containing a variety of pharmacologically active lignans has been traditionally used in oriental medicine. In this study, anti-inflammatory compounds were screened from the hexane extracts of S. chinensis by activity-guided fractionation. First, we investigated the regulatory effects on the expression of E-selectin, intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) with 38 fractions from the hexane extracts of S. chinensis in human umbilical vein endothelial cells (HUVECs). As a result, SCKH1 among the 38 fractions from the hexane extract of S. chinensis was selected for further analysis based on its unique regulatory effect on cell adhesion molecules, especially on VCAM-1, in LPS-stimulated HUVECs. The subsequent activity-guided fractionation of SCKH1 resulted in the purification of SCKH1PAIBPB, which was found to suppress the expression of VCAM-1, MCP-1, IL-6 and IL-8 in HUVECs stimulated with LPS, and to inhibit the adhesive capacity between HUVECs and monocytes. Taken together, our data indicate that SCKH1PAIBPB can be proposed as an effective anti-inflammatory compound that may have a potential therapeutic use for the prevention and treatment of various inflammatory diseases as well as ischemic vascular diseases.

예로부터 한방이나 민간요법에서 다양한 약리효능을 가진 약제로 알려져 있는 오미자 열매로부터 항염증 활성을 갖는 유용한 물질을 동정하고자 본 연구를 수행하였다. 먼저 오미자의 hexane 추출물로부터 38개의 분획물을 분리한 다음 각 분획의 항염증 활성을 측정하였다. 그 결과 오미자 분획물 중 생리활성이 높은 SCKH1을 선택하여 활성분획 추적방법을 통해 SCKH1PAIBPB을 분리하였다. SCKH1PAIBPB는 VCAM-1, MCP-1, IL-6 및 IL-8의 발현을 감소시키며, 혈관내피세포와 단핵구 사이의 부착능을 억제시킨다는 사실을 확인할 수 있었다. 따라서 본 연구를 통해 규명된 오미자 분획물 및 SCKH1PAIBPB의 항염증효과 뿐만 아니라 혈관내피세포 증식 및 생존능 촉진작용을 응용하여, 다양한 허혈성질환 뿐만 아니라 염중성질환의 예방 및 치료에 활용할 수 있을 것으로 사료된다.

Keywords

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