Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Effect of Glucose on Swarming Motility of Paenibacillus sp. CK214

Paenibacillus sp. CK214의 swarming 운동성에 미치는 glucose의 영향

  • Kang, Sung Wan (Department of Microbiology, Pusan National University) ;
  • Yoo, Ah Young (Department of Microbiology, Pusan National University) ;
  • Kang, Ho Young (Department of Microbiology, Pusan National University)
  • 강성완 (부산대학교 생명과학부 미생물학과) ;
  • 유아영 (부산대학교 생명과학부 미생물학과) ;
  • 강호영 (부산대학교 생명과학부 미생물학과)
  • Received : 2013.02.15
  • Accepted : 2013.02.18
  • Published : 2013.02.28
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Abstract

Paenibacillus is a gram-positive, spore-forming aerobes that was previously classified as a Bacillus species. Paenibacillus sp. CK214 was highly motile on LB agar plates and showed typical colonial morphology of Paenibacillus. However, its motility was defective in the absence of glucose. Electron microscopic observation revealed that the cells of CK214 cultured on LB agar plates were peritrichously flagellated but not flagellated in the presence of glucose. Flagellar filaments were purified by centrifugation after shearing off from the CK214 cells with vigorous pipetting. The purified protein was composed of a single flagellin with an apparent molecular size of 29 kDa. Recognition of the protein by anti-Edwardsiella tarda flagellin protein antibody demonstrates that the protein is a flagellin protein. A decreased level of flagellin protein was detected in CK214 cells grown under glucose-supplemented media.

Paenibacillus는 호기성의 내생포자를 형성하는 그람양성균으로써 이전에는 Bacillus로 분류되었다. Paenibacillus sp. CK214 균주는 LB agar 평판배지에서 높은 swarming 운동 능력을 가지고 Paenibacillus 특유의 집락 형태를 나타내었지만 glucose가 첨가된 평판배지에서는 운동 능력을 상실하였다. 투과전자현미경(TEM)을 이용하여 glucose 조건에 따른 CK214 균주의 편모를 관찰하면 LB agar 평판배지에서 배양한 CK214 균주는 주모성의 편모를 가지는 반면 glucose를 첨가한 평판배지에서 배양한 CK214 균주의 경우 주모성 편모가 나타나지 않는 것을 확인할 수 있었다. 물리적 충격과 원심분리를 통해 분리한 CK214 균주의 filament 구성 단백질을 SDS-PAGE를 통해 확인하였으며, 약 29 kDa 크기의 단일 단백질 밴드가 나타났다. Edwardsiella tarda 균주의 flagellin 단백질에 특이적인 항체를 이용한 immunoblotting 수행 결과, 이 단일 단백질 밴드는 flagellin 단백질임이 확인되었다. Glucose조건에 따른 CK214 균주의 flagellin 단백질의 발현을 단백질 수준에서 관찰한 결과, glucose가 첨가된 조건에서 생장한 CK214 균주에서의 flagellin 단백질 발현이 glucose가 없는 조건일 때에 비해 감소하는 것을 확인할 수 있었다.

Keywords

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