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Isolation and Characterization of Lactic Acid Bacteria from Kimchi, Korean Traditional Fermented Food to Apply into Fermented Dairy Products

  • 투고 : 2012.12.20
  • 심사 : 2013.02.07
  • 발행 : 2013.02.28

초록

This study aimed to isolate lactic acid bacteria (LAB) from Kimchi and to identify suitable probiotic strain for application in fermented dairy product as a commercial starter culture. A total of 106 (LAB) strains were isolated from Kimchi collected from different regions in Korea and their phenotypic characteristics were assayed. Four isolates from MRS agar plates were selected and designated as DKL109, DKL119, DKL121 and DKL128. They were identified first by API 50 CHL kit and then 16S rRNA gene sequencing. DKL121 and DKL128 were identified as Lactobacillus paracasei and Lactobacillus casei, respectively. Other two isolates (DKL109 and DKL119) were identified as Lactobacillus plantarum. To estimate their applicability in dairy products, the characteristics including acid and bile tolerance, cold shock induced cryotolerance and enzymatic activities were determined. There was wide variation in ability of strains to acid tolerance, but no significant differences in bile tolerance, cold shock induced cryotolerance within selected strains. DKL119 and DKL121 showed the highest resistance to acid and bile and the highest ${\beta}$-galactosidase activity, respectively. When these two strains were used for yogurt preparation as a single starter culture, their viable cell counts reached to $1.0{\times}10^9CFU/mL$. Lactobacillus plantarum DKL119 showed faster acid development than commercial starter culture. Also storage trials at $10^{\circ}C$ showed that the viability of these strains was retained over 15 d. With these results, it was indicated that probiotics isolated from Kimchi can be used in yogurt manufacturing as a starter culture.

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참고문헌

  1. Bamidele, T. A., B. A. Adeniyi, S. T. Ogunbanwo, S. I. Smith, and Omonigbehin. E. A. (2011) Antibacterial Activities of Lactic Acid Bacteria Isolated from Selected Vegetables Grown in Nigeria: A Preliminary Report. Sierra Leone J. Biomedical Res. 3, 128-132.
  2. Gandhi, D. N. (2007) Microbiology of Fermented Dairy Products. Food and Industrial Microbiology. National Science Digital Library at NISCAIR, India, pp. 1-31
  3. Gerez, C. L., Font de Valdez, G., Gigante, M. L., and Grosso, C. R. F. (2012) Whey protein coating bead improves the survival of the probiotic Lactobacillus rhamonosus CRL 1505 to low pH. Lett. Appl. Microbiol. 54, 552-556. https://doi.org/10.1111/j.1472-765X.2012.03247.x
  4. Heller, K. J. (2001) Probiotic bacteria in fermented foods: product characteristics and starter organisms. Am. J. Clin. Nutr. 73, 374S-379S. https://doi.org/10.1093/ajcn/73.2.374s
  5. Jalili, H., Razavi, S. H., Safari, M., and Malcata, F. X. (2009) Enhancement of growth rate and $\beta$-galactosidase activity, and variation in organic acid profile of Bifidobacterium animalis subsp. lactis Bb12. Enzyme Microbial Technol. 45, 469-476. https://doi.org/10.1016/j.enzmictec.2009.08.016
  6. Kim, B., Lee, J., Jang, J., Kim, J., and Han, H. (2003) Leuconostoc inhae sp. nov., a alctic acid bacterium isolated from kimchi. Int. J. Syst. Evol. Microbiol. 53, 1123-1126. https://doi.org/10.1099/ijs.0.02463-0
  7. Kim, C. H. and Kwak, H. S. (1993) Comparison of HPLC method with Bertrand and Lane-Eynon methods for the quantitative analysis of lactose in milk. Korean J. Dairy Sci. 15, 203-208.
  8. Kim, W. S. and Dunn, N. W. (1997) Identification of a cold shock gene in lactic acid bacteria and the effect of cold shock on cryotolerance. Curr. Microbiol. 35, 59-63. https://doi.org/10.1007/s002849900212
  9. Lee, H., Yoon, H., Ji, Y., Kim, H., Park, H., Lee, J., Shin, H., and Holzapfel, W. (2011) Functional properties of Lactobacillus strains isolated from kimchi. Int. J. Food Microbiol. 145, 155-161. https://doi.org/10.1016/j.ijfoodmicro.2010.12.003
  10. Lee, K. H. Park, J. Y., Jeong, S. J., Kwon, G. H., Lee, H. J., Chang, H. C., Chung, D. K., Lee, J. H., and Kim, J. H. (2007) Characterization of paraplantaricin C7, a novel bacteriocin produced by Lactobacillus paraplantarum C7 isolated from Kimchi. J. Microbiol. Biotechnol. 17, 287-296.
  11. Lee, S. G., Lee, K. W., Park, T. H., Park, J. Y., Han, N. S., and Kim, J. H. (2012) Proteomic analysis of proteins increased or reduced by ethanol of Lactobacillus plantarum ST4 isolated from Makgeolli, traditional Korean rice wine. J. Microbiol. Biotechnol. 22, 516-525. https://doi.org/10.4014/jmb.1109.09012
  12. Liang, Z. Q., Srinivasan, S., Kim, Y. J., Kim, H. B., Wang, H. T., and Yang, D. C. (2011) Lactobacillus kimchicus sp. nov., a $\beta$-galactosidases producing bacterium isolated from kimchi. Int. J. Syst. Evol. Microbiol. 61, 894-897. https://doi.org/10.1099/ijs.0.017418-0
  13. Lim, S. M. and Im, D. S. (2009) Screening and characterization of probiotic lactic acid bacteria isolated from Korean fermented foods. J. Microbiol. Biotechnol. 19, 178-186. https://doi.org/10.4014/jmb.0804.269
  14. Mishra, V. and Prasad, D. N. (2005) Application of in vitro methods for selection of Lactobacillus casei strains as potential probiotics. Int. J. Food Microbiol. 103, 109-115. https://doi.org/10.1016/j.ijfoodmicro.2004.10.047
  15. Prasad, J., Gill, H., Smart, J., and Gopal, P. K. (1998) Selection and characterization of Lactobacillus and Bifidobacterium strains for use as probiotic. Int. Dairy J. 8, 993-1002. https://doi.org/10.1016/S0958-6946(99)00024-2
  16. Richmond, M. L., Harte, B. R., Gray, J. I., and Stine, C. M. (1987) Determination of sugars in yogurt and microbiological media by high performance liquid chromatography during processing and subsequent storage. J. Dairy Sci. 70, 1140-1147 https://doi.org/10.3168/jds.S0022-0302(87)80124-8
  17. Rhimi, M. R., Aghajari, N., Jaouadi, B., Juy, M., Boudebbouze, S., Maguin, E., Haser, R., and Bejar, S. (2009) Exploring the acidotolerance of $\beta$-galactosidase from Lactobacillus delbrueckii subsp. bulgaricus: an attractive enzyme for lactose bioconversion. Res. Microbiol. 160, 775-784. https://doi.org/10.1016/j.resmic.2009.09.004
  18. Riffel, A., and Brandelli, A. (2006) Keratinolytic bacteria isolated from feather waste. Brazilian J. Microbiol. 37, 395-399. https://doi.org/10.1590/S1517-83822006000300036
  19. Rivals, J. P., Beal, C., Thammavongs, B., Gueguen, M., and Panoff, J. M. (2007) Cryotolerance of Lactobacillus delbrueckii subsp. bulgaricus CFL1 is modified by acquisition of antibiotic resistance. Cryobiology 55, 19-26. https://doi.org/10.1016/j.cryobiol.2007.04.003
  20. Rivera-Espinoza, Y. and Gallardo-Navarro, Y. (2010) Nondairy probiotic products. Food Microbiol. 27, 1-11. https://doi.org/10.1016/j.fm.2008.06.008
  21. Shin, M. S., Han, S. K., Ryu, J. S., Kim, K. S., and Lee, W. K. (2008) Isolation and partial characterization of a bacteriocin produced by Pediococcus pentosaceus K23-2 isolated from Kimchi. J. Appl. Microbiol. 105, 331-339. https://doi.org/10.1111/j.1365-2672.2008.03770.x
  22. Succi, M., Tremonte, P., Reale, A., Sorrentino, E., Grazia, L., Pacifico, S., and Coppola, R. (2005) Bile salt and acid tolerance of Lactobacillus rhamnosus strains isolated from Parmigiano Reggiano cheese. FEMS Microbiol. Lett. 244, 129-137. https://doi.org/10.1016/j.femsle.2005.01.037
  23. Yang, E. J. and Chang, H. C. (2009) Analysis of pYC2, a cryptic plasmid in Lactobacillus sakei BM5 isolated from kimchi. Biotechnol. Lett. 31, 123-130. https://doi.org/10.1007/s10529-008-9842-y

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  1. Characterization and Antibacterial Potential of Lactic Acid Bacterium Pediococcus pentosaceus 4I1 Isolated from Freshwater Fish Zacco koreanus vol.7, 2016, https://doi.org/10.3389/fmicb.2016.02037
  2. Assessment of Characteristics and Functional Properties of Lactobacillus Species Isolated from Kimchi for Dairy Use vol.35, pp.3, 2015, https://doi.org/10.5851/kosfa.2015.35.3.339
  3. Microencapsulation of Lactobacillus plantarum DKL 109 using External Ionic Gelation Method vol.34, pp.5, 2014, https://doi.org/10.5851/kosfa.2014.34.5.692
  4. Characterization and pharmacological potential of Lactobacillus sakei 1I1 isolated from fresh water fish Zacco koreanus vol.24, pp.1, 2016, https://doi.org/10.1186/s40199-016-0147-8
  5. Bacterial Diversity and Mycotoxin Reduction During Maize Fermentation (Steeping) for Ogi Production vol.6, 2015, https://doi.org/10.3389/fmicb.2015.01402
  6. A lactic acid bacterium isolated from kimchi ameliorates intestinal inflammation in DSS-induced colitis vol.55, pp.4, 2017, https://doi.org/10.1007/s12275-017-6447-y
  7. Characterization and Antibacterial Mode of Action of Lactic Acid BacteriumLeuconostoc mesenteroidesHJ69 from Kimchi vol.41, pp.1, 2017, https://doi.org/10.1111/jfbc.12290
  8. Lactobacillus plantarum DK119 as a Probiotic Confers Protection against Influenza Virus by Modulating Innate Immunity vol.8, pp.10, 2013, https://doi.org/10.1371/journal.pone.0075368
  9. Production of Functional High-protein Beverage Fermented with Lactic Acid Bacteria Isolated from Korean Traditional Fermented Food vol.35, pp.2, 2015, https://doi.org/10.5851/kosfa.2015.35.2.189
  10. Characterization of Lactobacillus plantarum strains isolated from black raspberry and their effect on BALB/c mice gut microbiota pp.2092-6456, 2018, https://doi.org/10.1007/s10068-018-0420-3
  11. Microencapsulation of endophytic LAB (KCC-41) and its probiotic and fermentative potential for cabbage kimchi vol.22, pp.1, 2019, https://doi.org/10.1007/s10123-018-00034-4
  12. Heat-killed Lactobacillus casei confers broad protection against influenza A virus primary infection and develops heterosubtypic immunity against future secondary infection vol.7, pp.None, 2013, https://doi.org/10.1038/s41598-017-17487-8
  13. Nitroso-hemoglobin Increased the Color Stability and Inhibited the Pathogenic Bacteria in a Minced Beef Model: A Combined Low-field NMR Study vol.39, pp.5, 2013, https://doi.org/10.5851/kosfa.2019.e40
  14. Efficacy and Safety of New Lactobacilli Probiotics for Unconstipated Irritable Bowel Syndrome: A Randomized, Double-Blind, Placebo-Controlled Trial vol.11, pp.12, 2013, https://doi.org/10.3390/nu11122887
  15. Effects of Novel Probiotics in a Murine Model of Irritable Bowel Syndrome vol.75, pp.3, 2013, https://doi.org/10.4166/kjg.2020.75.3.141
  16. A high-throughput system for screening high diacetyl-producing lactic acid bacteria in fermented milk in 96-well microplates vol.14, pp.1, 2020, https://doi.org/10.1007/s11694-019-00321-2
  17. Inhibitory effect of lactic acid bacteria isolated from kimchi against murine norovirus vol.109, pp.None, 2020, https://doi.org/10.1016/j.foodcont.2019.106881
  18. The effects of three novel probiotics isolated from the Korean fermented food Kimchi on the stress-induced defecation of rats vol.4, pp.1, 2013, https://doi.org/10.23838/pfm.2019.00121
  19. Lactic Acid Bacteria Isolated From Korean Kimchi Activate the Vitamin D Receptor-autophagy Signaling Pathways vol.26, pp.8, 2013, https://doi.org/10.1093/ibd/izaa049
  20. Adjuvant effects of killed Lactobacillus casei DK128 on enhancing T helper type 1 immune responses and the efficacy of influenza vaccination in normal and CD4-deficient mice vol.38, pp.36, 2013, https://doi.org/10.1016/j.vaccine.2020.06.075
  21. Probiotic Delivery through Non-Dairy Plant-Based Food Matrices vol.11, pp.7, 2013, https://doi.org/10.3390/agriculture11070599