Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Antioxidant and Anti-adipogenic Effects of PineXol®

PineXol®의 항산화 및 지방세포 분화 억제 효과

  • Lee, Young Jun (Department of Food Science and Biotechnology, Kangwon National University) ;
  • Han, Ohan Taek (Nutrapham Co., Ltd.) ;
  • Choi, Hyeon-Son (Department of Food Science and Biotechnology, CHA University) ;
  • Lee, Boo Yong (Department of Food Science and Biotechnology, CHA University) ;
  • Chung, Hyun-Jung (Department of Food and Nutrition, Inha University) ;
  • Lee, Ok-Hwan (Department of Food Science and Biotechnology, Kangwon National University)
  • 이영준 (강원대학교 식품생명공학과) ;
  • 한완택 ((주)뉴트라팜) ;
  • 최현선 (차의과학대학교 식품생명공학과) ;
  • 이부용 (차의과학대학교 식품생명공학과) ;
  • 정현정 (인하대학교 식품영양학과) ;
  • 이옥환 (강원대학교 식품생명공학과)
  • Received : 2012.11.06
  • Accepted : 2012.12.11
  • Published : 2013.02.28
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Abstract

Pine bark extract is made from the bark of Pinus densiflora which naturally contains occurring phytochemicals such as phenolic compounds. PineXol$^{(R)}$ from products of pine bark extract is sold under the brand name. The aim of this study was to evaluate the total phenol, total flavonoids contents and antioxidant activity of the PineXol$^{(R)}$ as well as to assess the lipid accumulation during adipogenesis of 3T3-L1 cells. Our results demonstrate that the total phenolic and flavonoids contents of the PineXol$^{(R)}$ were $717.40{\pm}6.86$ GAE mg/mL and $54.44{\pm}0.01$ RE mg/mL, respectively. The antioxidative activities of the PineXol$^{(R)}$ were significantly increased in a dose dependent manner on DPPH (1,1-Diphenyl-2-picryl hydrazyl) radical scavenging, ABTS (2,2'-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt) radical scavenging, FRAP (ferric reducing antioxidant power) activity, reducing power, nitrite radical scavenging activity and ORAC (Oxygen radical absorbance capacity) value. In addition, the PineXol$^{(R)}$ inhibited the adipocyte differentiation of 3T3-L1 preadipocytes. Exposure to 200 ${\mu}g/mL$, PineXol$^{(R)}$ significantly reduced lipid accumulation (~80%) in 3T3-L1 cells compared to control cells.

소나무 껍질 추출물은 높은 항산화 활성을 가지는 것으로 많은 연구 결과를 통해 조사되어 왔으며, 해외에서는 이미 프랑스 해송 껍질 추출물인 피크노제놀(pycnogenol)에 관한 연구가 많이 이루어진 반면, 우리나라 적송 껍질 추출물(PineXol$^{(R)}$)의 생리활성 효과에 대해서는 아직 연구가 많이 이루어지지 못했다. 따라서 본 연구에서는 우리나라 적송 껍질 추출물인 PineXol$^{(R)}$의 항산화 활성 및 anti-adipogenic 활성을 평가하였다. PineXol$^{(R)}$의 총 페놀 및 플라보노이드 함량은 각각 $717.40{\pm}6.86$ GAE mg/g 및 $54.44{\pm}0.01$ RE mg/g으로 측정되었다. 또한 다양한 항산화 평가 모델(DPPH, ABTS, FRAP, 환원력)을 통하여 PineXol$^{(R)}$의 항산화 활성을 측정한 결과, 농도가 증가함에 따라 항산화 활성이 유의적으로 증가하였으며, 대조군으로 사용한 동일한 농도의 아스코르빈산과 유사한 항산화 활성을 나타내었다. 또한 ORAC value는 $693.97{\pm}14.13{\mu}M$ TE/g으로 측정되었고, 1.0 mg/mL의 농도에서 55.39%의 아질산염소거능을 나타내었다. PineXol$^{(R)}$은 3T3-L1 지방세포에서 세포독성을 나타내지 않았으며, 분화과정동안 50, 100 및 200 ${\mu}g/mL$의 농도에서의 지방축적량은 각각 $66.85{\pm}5.87$, $44.59{\pm}5.71$ 및 $20.85{\pm}2.78%$의 농도가 증가함에 따른 유의적인 억제효과를 보였다. 이상의 결과로부터, PineXol$^{(R)}$은 항산화 활성 및 지방세포 분화억제 효능을 갖으며, 천연물 유래 항산화제로써 활용 가능성이 높은 것으로 기대된다.

Keywords

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