Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
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Soil Acclimatization of Regenerated Plants by Gibberellic Acid Treatments of Panax ginseng C. A. Meyer

GA3 처리에 의한 인삼 재분화 식물체의 토양 순화

  • Kim, Young Chang (Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Park, Hong Woo (Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Kim, Ok Tae (Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Bang, Kyong Hwan (Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Kim, Jang Uk (Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Hyun, Dong Yun (Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Kim, Dong Hwi (Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Cha, Seon Woo (Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Choi, Jae Eul (College of Agriculture ＆ Life Sciences, Chungnam National University)
  • 김영창 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 박홍우 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 김옥태 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 방경환 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 김장욱 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 현동윤 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 김동휘 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 차선우 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 최재을 (충남대학교 농업생명과학대학 식물자원학과)
  • Received : 2012.07.10
  • Accepted : 2013.02.04
  • Published : 2013.02.28
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Abstract

We established that regenerated plants of Panax ginseng were directly transplanted into the soil without the acclimatization process. When regenerated plants were treated at the concentration of $0.4mg\;l^{-1}$ for 9 hr before transplantation into the soil, our results showed that the survival rate of them was the highest. The optimal size of the root was above 4 cm in length and 0.4 g by weight before transplantation into the soil. The highest survival rate increased to 59.6%. After 4 years of cultivation in the fields, our results showed that there were no significant morphological differences between regenerated plants and wild-type ginseng. Also, we obtained healthy seeds from regenerated plants. This study provides the basis for a relatively rapid multiplication of selected genotypes and will allow a much more rapid evaluation of the germplasm of the unexplored and unstudied Korean ginseng.

본 연구는 인삼 기내에서 생산된 재분화 식물체의 토양 순화를 위해 순화과정을 단축하고 생존율을 높일 수 있는 방법을 개발하기 위하여 실시하였다. $GA_3$를 $0.4mg\;l^{-1}$로 9시간을 처리하였을 때 토양에서 생존율은 59.6%로 가장 높게 나타났으며, 그 다음이 $1mg\;l^{-1}$에서 9시간을 처리하였 때 43.7%의 생존율을 나타냈다. 재분화 식물체의 뿌리길이가 4 cm 이상일 때는 48개체가 생존하여 생존율은 53.3%로 처리 중 가장 높게 나타났으며, 뿌리 무게는 4 g 이상일때는 46.7%가 생존하여 가장 높은 비율을 나타냈다. 4년생 때 지상부 생육 특성을 조사한 결과, 초장은 35.3 cm, 경장은 18.3 cm, 엽장은 12.1 cm, 엽폭은 4.8 cm로 이식 재배한 연풍의 생육 특성과 비교해 보면 재분화 식물체의 생육이 약간 떨어짐을 볼 수 있었다. 3년생 때 지하부 특성을 보면 근장은 15.3 cm, 동장은 4.5 cm, 동직경은 2.1 cm, 그리고 근중은 15.4 g으로 지하부도 지상부와 마찬가지로 대조구인 묘삼을 이식하여 재배한 처리구에 비하여 생육이 약간 떨어졌다.

Keywords

References

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