DOI QR코드

DOI QR Code

Identification of Fungal Pathogen Causing Seedling Rot of Lithospermum erythrorhizon and Study on the Optimum Growing Temperature for Decreasing of the Seedling Rot

지치 육묘시 발생하는 유묘썩음증상 원인균 분리·동정 및 경감 육묘환경에 관한 연구

  • An, Tae Jin (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Shin, Kyu Seop (College of Agricultural and Life Sciences, Chungnam National University) ;
  • Ahn, Young Sup (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Hur, Mok (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Park, Chung Berm (Department of Herbal Crop Research, NIHHS, RDA)
  • 안태진 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 신규섭 (충남대학교 농업생명과학대학 농생물학과) ;
  • 안영섭 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 허목 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 박충범 (농촌진흥청 국립원예특작과학원 인삼특작부)
  • Received : 2012.11.16
  • Accepted : 2013.01.18
  • Published : 2013.02.28

Abstract

This study was conducted to isolate and identify the fungal pathogen causing seedling rot of Lithospermum erythrorhizon Siebold & Zuccarini, and to know the optimum growing temperature for decreasing seedling rot of Lithospermum erythrorhizon. On the basis of morphological characteristics, EF-1a sequence analysis, and pathogenecity to host plant, the fungi isolated from seedling rot and seeds of Lithospermum erythrorhizon were identified as Fusarium fujikuroi, indicating that disease causing fungus is seed-borne pathogen. Optimum temperature for germination of seeds of Lithospermum erythrorhizon was $15{\sim}20^{\circ}C$, but pathogenicity of Fusarium fujikuroi was shown more readily at $25{\sim}30^{\circ}C$. These results suggested that seedling culture of Lithospermum erythrorhizon between $15^{\circ}C$ and $20^{\circ}C$ might reduce seedling rot of Lithospermum erythrorhizon caused by seed-borne pathogen Fusarium fujikuroi.

Keywords

References

  1. Bae JH. (2004). Antimicrobial effect of Lithospermum erythrorhizon extracts on the food-borne pathogens. Korean Journal of Food Science and Technology. 36:823-827.
  2. Kim JS, Kim TY and Kim SB. (2006). Evaluation of storage characteristics of Kangjung added with gromwell extracts. The Korean Journal of Food and Nutrition. 35:791-800. https://doi.org/10.3746/jkfn.2006.35.6.791
  3. Kim GS, Jeong TS, Kwon BM, Kim YO, Cha SW, Song KS and Baek NI. (2009). Inhibitory effect of acetylshikonin from roots of Lithospermum erythrorhizon on LDL oxidation and FPTase activity. Journal of the Korean Society for Applied Biological Chemistry. 52:221-225. https://doi.org/10.3839/jabc.2009.038
  4. Kim JS and Kang NH. (2010). Antioxidant activity of solvent fractions from cultivated and wild gromwell. The Korean Journal of Food and Nutrition. 39:789-795. https://doi.org/10.3746/jkfn.2010.39.6.789
  5. Kim JS, Jeong MH, Choi WY, Seo YC, MA CJ, Ahyn JH, Kim NS, Hwang B, Cho JS and Lee HY. (2011). Enhancement of whitening effects of Lithospermum erythrorhizon extracts by ultra high pressure. Korean Journal of Medicinal Crop Science. 19:97-102. https://doi.org/10.7783/KJMCS.2011.19.2.097
  6. Kim GS, Park CG, Lee KH, Choi JH, Lee SE, Noh HJ, Lee JH and Kim SY. (2011). Investigation of shikonin pigments and antioxidant activity of the roots from Lithospermum erythrorhizon according to the different growth stages and areas of cultivation. Korean Journal of Medicinal Crop Science. 19:435-440. https://doi.org/10.7783/KJMCS.2011.19.6.435
  7. Lee EJ, Lee YH, Cho WD, Kim WG and Jin KS. (1991). Compendium of medicinal plant diseases with colour plates. National Institute of Agricultural Science and Technology. Suwon, Korea. p.105-107.
  8. Lee HH, Bai S and Chin JE. (2005). Inhibitory effect of Lithospermum erythrorhizon extracts on melanin biosynthesis. The Korean Journal of Food and Nutrition. 34:1325-1329. https://doi.org/10.3746/jkfn.2005.34.9.1325
  9. Leslie JF and Summerell BA. (2006). The Fusarium laboratory manual. Blackwell Publishing. Ames. Iowa, USA. p.172-173.
  10. Seo YC, Choi Wy, Kim JS, Cho JS, Kim YO, Kim JC and Lee HY. (2011). Enhancement of anticancer activities from Lithospermum erythrorhizon extracts by ultra high pressure process. Korean Journal of Medicinal Crop Science. 19:103-110. https://doi.org/10.7783/KJMCS.2011.19.2.103
  11. Stamatakis A. (2008). RAxML-VI-HPC: Maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models. Bioinformatics. 22:2688-2690.
  12. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F and Higgins DG. (1997). The CLUSTAL X windows interface: Flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Resources. 25:4876-4882. https://doi.org/10.1093/nar/25.24.4876
  13. Watanabe M, Yonezawa T, Lee K, Kumagai S, Sugita-Konishi Y, Goto K and Hara-Kudo Y. (2011). Molecular phylogeny of the higher and lower taxonomy of the Fusarium genus and differences in the evolutionary histories of multiple genes. BMC Evolutionary Biology. 11:322. https://doi.org/10.1186/1471-2148-11-322
  14. Watanabe M, Goto K, Sugita-Konishi Y, Kamata Y and Hara-Kudo Y. (2012). Sensitive detection of whole-genome differentiation among closely-related species of the genus Fusarium using DNADNA hybridization and a microplate technique. The Journal of Veterinary Medical Science. 74:1333-1336. https://doi.org/10.1292/jvms.12-0034

Cited by

  1. Studies on Seed Germination Characteristics and Patterns of Protein Expression of Lithospermum erythrorhizon by Plant Growth Regulators and Seed Primings vol.22, pp.6, 2014, https://doi.org/10.7783/KJMCS.2014.22.6.435
  2. The Mechanism Underlying the Antibacterial Activity of Shikonin against Methicillin-ResistantStaphylococcus aureus vol.2015, 2015, https://doi.org/10.1155/2015/520578
  3. Effect of Irrigation of Sulfur Solution before Sowing on Growth and Root Rot Disease of Seedling in Ginseng Nursery vol.22, pp.5, 2014, https://doi.org/10.7783/KJMCS.2014.22.5.391