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Induction of Potent Antigen-specific Cytotoxic T Cell Response by PLGA-nanoparticles Containing Antigen and TLR Agonist

  • Lee, Young-Ran (College of Pharmacy, Chungbuk National University) ;
  • Lee, Young-Hee (College of Pharmacy, Chungbuk National University) ;
  • Kim, Ki-Hyang (College of Pharmacy, Chungbuk National University) ;
  • Im, Sun-A (College of Pharmacy, Chungbuk National University) ;
  • Lee, Chong-Kil (College of Pharmacy, Chungbuk National University)
  • Received : 2013.01.18
  • Accepted : 2013.02.04
  • Published : 2013.02.28
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Abstract

Previously we showed that biodegradable nanoparticles containing poly-IC or CpG oligodeoxynucleotide (ODN) together with ovalbumin (OVA) were efficient at inducing MHC-restricted presentation of OVA peptides in dendritic cells. The CTL-inducing activities of the nanoparticles were examined in the present study. Nanoparticles containing poly-IC or CpG ODN together with OVA were prepared using biodegradable polymer poly(D,L-lactic acid-co-glycolic acid), and then were opsonized with mouse IgG. The nanoparticles were injected into the tail vein of mice, and 7 days later the OVA-specific CTL activities were measured using an in vivo CTL assay. Immunization of mice with the nanoparticles containing poly-IC or CpG ODN together with OVA elicited potent OVA-specific CTL activity compared to those containing OVA only. In accordance with these results, nanoparticles containing poly-IC or CpG ODN together with OVA exerted potent antitumor activity in mice that were subcutaneously implanted with EG7.OVA tumor cells. These results show that encapsulation of poly-IC or CpG ODN together with antigen in biodegradable nanoparticles is an effective approach for the induction of potent antigen-specific CTL responses in vivo.

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References

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