KOREAN JOURNAL OF CROP SCIENCE (한국작물학회지)

The Korean Society of Crop Science (한국작물학회)
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Cultivar Comparison on Tocopherols, Tocotrienols, and Antioxidant Compounds in Rice Bran

미강의 토코페롤 및 토코트리에놀 함량과 항산화 물질의 품종간 비교

  • Chun, Areum (National Institute of Crop Science, Rural Development Administration) ;
  • Lee, Yoo-Young (National Institute of Crop Science, Rural Development Administration) ;
  • Kim, Dae-Jung (National Institute of Crop Science, Rural Development Administration) ;
  • Yoon, Mi-Ra (National Institute of Crop Science, Rural Development Administration) ;
  • Oh, Sea-Kwan (National Institute of Crop Science, Rural Development Administration) ;
  • Choi, Im-Soo (National Institute of Crop Science, Rural Development Administration) ;
  • Hong, Ha-Cheol (National Institute of Crop Science, Rural Development Administration)
  • 천아름 (농촌진흥청 국립식량과학원) ;
  • 이유영 (농촌진흥청 국립식량과학원) ;
  • 김대중 (농촌진흥청 국립식량과학원) ;
  • 윤미라 (농촌진흥청 국립식량과학원) ;
  • 오세관 (농촌진흥청 국립식량과학원) ;
  • 최임수 (농촌진흥청 국립식량과학원) ;
  • 홍하철 (농촌진흥청 국립식량과학원)
  • Received : 2013.07.12
  • Accepted : 2013.11.27
  • Published : 2013.12.30
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Abstract

The rice bran, a by-product of rice milling process, is well known for various functional components, such as tocopherol, tocotrienol, ${\gamma}$-oryzanol, carrying antioxidant activities. This study was conducted to investigate the antioxidant components and antioxidant activities in rice bran of different Korean rice cultivars. The 8 isomers of vitamin E, ${\gamma}$-oryzanol, flavonoids, and polyphenolics in rice bran from 16 Korean premium and high quality rice cultivars were quantified. DPPH and ABTS radical scavenging activities and reducing power of the ethanol extracts of rice bran were measured. 'Hopum' showed the highest total vitamin E content, $221.47{\mu}g/g$ among the cultivars, and 'Hanseol' showed the lowest content. The rice bran showed different compositions of ${\alpha}-$, ${\beta}-$, ${\gamma}-$, ${\delta}-$ tocopherol and tocotrienol among rice cultivars. The antioxidant contents were also different by cultivar; the ${\gamma}$-oryzanol contents ranged from 1.99 mg/g (Unkwang) to 4.30 mg/g (Chilbo), the polyphenol contents ranged from 427.22 mg gallic acid eq./100 g (Odaebyeo) to 775.80 mg gallic acid eq./100 g (Hopum). 'Hopum' also had the highest DPPH and ABTS free radical scavenging activities, 9.82% and 187.5 AEAC mg/100 g, respectively. In vitro, the rice bran extracts from 'Hopum' had significantly higher antioxidant activities than that of other cultivars.

본 연구는 국내 육성 벼 품종 미강의 토코페롤, 토코트리에놀, 폴리페놀, ${\gamma}$-oryzanol 함량 및 기타 항산화 활성의 차이를 검토한 결과이다. 1. 품종별 미강의 조지방 함량은 15.8%(오대)~23.9%(칠보)이었으며 단백질 함량은 12.2%(한설)~15.1%(조운), 회분 함량은 8.7%(추청)~10.8%(한설), 식이섬유 함량은 34.1%(삼광)~43.5%(청해진미)의 범위를 나타났다. 2. 품종별 미강의 전체 비타민 E 함량은 '호품'($221.47{\mu}g/g$), '칠보'($211.63{\mu}g/g$), '청해진미'($208.54{\mu}g/g$)순으로 높았고, '한설'($106.65{\mu}g/g$)이 가장 낮은 함량을 보였다. 토코페롤과 토코트리에놀은 각각 ${\alpha}$-Tocopherol과 ${\gamma}$-Tocotrienol이 주를 이루었으며, 밥쌀용 품종 미강의 비타민 E 이성체별 함량 분포가 품종에 따라 유의한 차이를 보였다. 3. ${\gamma}$-oryzanol은 '칠보'(4.30 mg/g)가 가장 높은 함량을 보였으며, 미강 에탄올 추출물의 폴리페놀 함량은 '호품'(775.80 mg gallic acid/100g)이 가장 높은 함량을 나타내었다. 플라보노이드 함량 등과 높은 연관을 보이는 DPPH 라디칼 소거능은 호품이 9.82%로 가장 높았으며, 폴리페놀 화합물 등과 관계되는 ABTS 라디칼 소거능과 환원력은 각각 '호품'(187.5 AEAC mg/100g), '운광'(A700 = 0.19)에서 가장 높은 함량을 나타내었다. 4. 밥쌀용 벼 품종 간 미강 기능성 성분 함량의 유의한 차이를 확인하였고, 미강의 이용성 증진을 위하여 주요 품종의 특성을 이해하고 기능성 성분의 함량, 활성 및 추출 수율을 최적화하기 위한 연구가 지속적으로 수행되어야 할 것으로 생각된다.

Keywords

References

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