YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Immune-stimulating Effects of Polygonum aviculare L. Extract on Macrophages

마디풀(Polygonum aviculare L.) 추출물의 대식구 면역증강 효과

  • Jeon, Chang Bae (Department of Biology Education, College of Education, Chungbuk National University) ;
  • Kim, Young Hoon (Department of Biology Education, College of Education, Chungbuk National University) ;
  • Batsuren, Dulamjav (Institutes of Chemistry and Chemical Technology, MAS) ;
  • Tunsag, Jigjidsuren (Institutes of Chemistry and Chemical Technology, MAS) ;
  • Nho, Chu Won (Functional Food Center, Korea Institute of Science and Technology) ;
  • Pan, Cheol-Ho (Functional Food Center, Korea Institute of Science and Technology) ;
  • Lee, Jae Kwon (Department of Biology Education, College of Education, Chungbuk National University)
  • 전창배 (충북대학교 사범대학 생물교육과) ;
  • 김영훈 (충북대학교 사범대학 생물교육과) ;
  • ;
  • ;
  • 노주원 (한국과학기술연구원 기능성천연물센터) ;
  • 판철호 (한국과학기술연구원 기능성천연물센터) ;
  • 이재권 (충북대학교 사범대학 생물교육과)
  • Received : 2013.09.07
  • Accepted : 2013.12.16
  • Published : 2013.12.31
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Abstract

In this study we demonstrated whether the extract of Polygonum aviculare L. (PAE) can be applied to the immune-stimulating responses in macrophages (Raw 264.7 cells). Cell viability was determined by WST-8 assay, and all four doses of PAE (5, 10, 20, and 40 ${\mu}g/ml$) had no significant cytotoxicity during the entire experimental period. PAE increased the production of inducible nitric oxide synthase (iNOS) and nitric oxide (NO), and mRNA expressions and protein levels of pro-inflammatory cytokines(tumor necrotic factor (TNF)-${\alpha}$, interleukin (IL)-$1{\beta}$ and IL-6) in the same cells. These immune-stimulating activities of PAE were found to be caused by the stimulation of $NF{\kappa}B$ signal and phosphorylation of MAP kinases (p38, ERK and JNK).

Keywords

References

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