DOI QR코드

DOI QR Code

Evaluation of Environment-friendly Control Agents for the Management of Powdery Mildew Infection during Seedling Stage of Three Cucurbitaceae Vegetables

친환경 육묘시 세 가지 박과채소의 흰가루병에 대한 친환경 제제의 방제효과

  • Yeo, Kyung-Hwan (Protected Horticulture Research Station, National Institute of Horticultural and Herbal Sciences, RDA) ;
  • Jang, Yoon Ah (Vegetable Research Division, National Institute of Horticultural and Herbal Sciences, RDA) ;
  • Kim, Su (Vegetable Research Division, National Institute of Horticultural and Herbal Sciences, RDA) ;
  • Um, Young Chul (Vegetable Research Division, National Institute of Horticultural and Herbal Sciences, RDA) ;
  • Lee, Sang Gyu (Vegetable Research Division, National Institute of Horticultural and Herbal Sciences, RDA) ;
  • Rhee, Han Cheol (Protected Horticulture Research Station, National Institute of Horticultural and Herbal Sciences, RDA)
  • 여경환 (농촌진흥청 국립원예특작과학원 시설원예시험장) ;
  • 장윤아 (농촌진흥청 국립원예특작과학원 채소과) ;
  • 김수 (농촌진흥청 국립원예특작과학원 채소과) ;
  • 엄영철 (농촌진흥청 국립원예특작과학원 채소과) ;
  • 이상규 (농촌진흥청 국립원예특작과학원 채소과) ;
  • 이한철 (농촌진흥청 국립원예특작과학원 시설원예시험장)
  • Received : 2013.09.15
  • Accepted : 2013.11.27
  • Published : 2013.12.31

Abstract

The purpose of this study was to evaluate the control efficacy of major environment-friendly control agents against powdery mildew, as affected by the application condition such as disease-developing stage and microclimate, as compared with the efficacy of pesticides in plug seedling of three Cucurbitaceae vegetables, including cucumber, melon, and oriental melon. Single or combined application of major six environment-friendly control agents was used in the experiment: two biofungicide (Ampelomyces quisqualis 94013 and Bacillus subtilis Y1336), two plant extracts (neem oil and extracts of Rheum undulatum), and two mineral materials (wettable sulfur powder and lime bordeaux mixture). These control agents were treated to the plug seedlings for preventing powdery mildew and curative applications for managing powdery mildew. In all treatments, the disease incidence declined as daily average temperatures increased to $30^{\circ}C$ for consecutive 6~8 days with maximum temperature over $40^{\circ}C$. In preventative application, the control efficacy against powdery mildew was the highest in the treatment of wettable sulfur powder, and lowest in the B. subtilis Y1336, with values of 20~40%. In cucumber seedlings, the preventive single application of neem oil or wettable sulfur powder was more effective than curative application of fungicides, while the control efficacy of these agents was similar to those of fungicides in melon seedlings. The single application of R. undulatum extracts was also effective in preventing the disease for both cucumber and melon seedlings, showing a higher control efficacy than those of biofungicides during seedling stage. The treatment of water spray was not effective and showed a higher disease incidence than the untreated control plot in the oriental melon and melon seedlings. The curative application with environment friendly control agents, after powdery mildew was first detected, could not successfully controlled the disease at the middle stage (5~10% of disease incidence) of disease development. The curative combined application of [R. undulatum extracts (1st application) + wettable sulfur powder (2nd) + neem oil (3rd)] showed the highest control efficacy among the other treatments, with control value over 80% at the early stage (less than 1% of disease incidence) of disease development.

본 실험은 박과류(오이, 참외, 멜론)를 공시작물로 하여 친환경 육묘시 친환경 제제와 시용조건에 따른 흰가루병의 방제효과를 구명하고 기존 등록된 농약과 대조하여 그 효과를 검정하기 위해 수행되었다. 친환경제제는 유효미생물로서 Ampelomyces quisqualis 94013(AQ94013)과 Bacillus subtilis Y1336(BS Y1336), 식물추출물로서 님오일(Neem oil)과 대황(Rheum undulatum)추출물, 미네랄 제제는 황수화제와 석회보르도액(Lime Bordeaux mixture)을 단용 또는 혼용처리하여 병 발생시기(발생전, 초기, 중기)에 따라 처리하였다. 모든 처리구에서 시설내 일 평균온도 $30^{\circ}C$ 이상, 하루 중 최고기온이 $40^{\circ}C$ 이상의 조건이 일주일 이상 지속되는 기간 동안 유묘의 흰가루병의 발병도는 감소하였고, 대조구인 무처리구에서의 발병도도 크게 감소되어 10% 미만으로 나타났다. 발생 전처리시 방제효과는 모든 작물에서 황수화제가 가장 높았고, BS Y1336는 방제가가 20~40%로서 가장 낮게 나타났다. 오이의 경우 발생전 처리시 님오일과 황수화제 처리는 살균제보다 높은 방제효과를 보였으며, 멜론에서는 농약과 비슷한 방제가를 보였다. 대황추출물은 전 생육기간 동안 미생물제제보다 높은 방제가를 나타내었고, 오이와 멜론작물에서 발생초기 처리시 효과가 우수하였다. 물살포구는 무처리구와 비슷한 발병도를 보여 방제효과가 없었고, 참외와 멜론에서 물살포 처리는 무처리보다 더 높은 발병도를 보였다. 병발생후 친환경제제 처리시 방제효과는 처리하는 시기가 늦어질수록 감소하였다. 친환경제제의 혼용처리에 의한 방제효과를 조사하였을 때, 대황추출물(1주차) + 황수화제(2주차) + 님오일(3주차)의 조합으로 한 가지의 친환경제제를 주당 1회씩 총 3주 동안 혼용처리 했을 때, 전 육묘기간 동안 오이에서는 90% 이상, 모든 작물에서 평균 80% 이상의 방제가를 나타내어 다른 조합의 처리에 비해 높은 방제효과를 보였다. 본 실험결과를 통해 박과채소의 친환경 육묘시 흰가루병에 대한 친환경제제의 방제효과는 단용처리 했을 때, 병발생 전후 처리에 상관없이 모든 작물에서 황수화제 처리가 가장 높게 나타났으며, 혼용처리시에는 대황추출물(1주차) + 황수화제(2주차) + 님오일(3주차)의 조합이 가장 효과가 높은 것으로 나타났다.

Keywords

References

  1. Elad, Y., Y. Messika, M. Brand, D.R. David, and A. Sztejnberg. 2007. Effect of microclimate on Leveillula taurica powdery mildew of sweet pepper. The American Phytopathological Society 97(7):813-824. https://doi.org/10.1094/PHYTO-97-7-0813
  2. Endo, T. 1989. Studies on the life-cycle of cucurbit powdery mildew fungus Sphaerotheca fuliginea (schlecht) Poll. Spec. Bull. Fukushima Pref. Agr. Exp. Stn. 5:1-106.
  3. Jeong, K.J., Y.S. Chon, S.H. Ha, H.K. Kang, and J.G. Yun. 2012. Silicon application on standard chrysanthemum alleviates damatges induced by disease and aphid insect. Kor. J. Hort. Sci. Technol. 30(1):21-26 (in Korean).
  4. Keinath, A.P. and V.B. DuBose. 2004. Evaluation of fungicides for prevention and management of powdery mildew on watermelon. Crop Prot. 23:35-42. https://doi.org/10.1016/S0261-2194(03)00165-0
  5. Kim, D.H., J.H. Park, J.S. Lee, K.S. Han, Y.K. Han, and J.H. Hwang. 2009. Effect of temperature, relative humidity on germination and development of powdery mildew (Leveillula taurica) on pepper and its inoculation method. Res. Plant Dis. 15(3):187-192 (in Korean). https://doi.org/10.5423/RPD.2009.15.3.187
  6. Koji, A. 1986. Host range and geographical distribution of the powdery mildew. Japan Societies Press, Tokyo, Japan.
  7. Lee, S.Y., J.D. Ryu, and .G. Kim. 2005. Environmental factors affecting parasitism to cucumber powdery mildew fungus, Sphaerotheca fusca by Ampelomyces quisqualis 94013 and its host range. Res. Plant Dis. 11(2):167-172 (in Korean). https://doi.org/10.5423/RPD.2005.11.2.167
  8. Lee, O.H., H.S. Hwang, J.Y. Kim, J.H. Han, Y.S. Yoo, and B.S. Kim. 2001. A search for sources for sources of resistance to powdery mildew (Leveillula taurica (Lév.) Arn) in pepper (Capsicum spp.). Kor. J. Hort. Sci. Technol. 19:7-11 (in Korean).
  9. Nam, M.H., S.K. Jung, S.W. Ra, and H.G. Kim. 2003. Control efficacy of sodium bicarbonate alone and in mixture with polyoxyethylene sorbitanmonolaurate on powdery mildew of strawberry. Kor. J. Hort. Sci. 21(2):98-101 (in Korean).
  10. Nam, M., J. Choi, H.J. Kim, J. Lee, K. Lim, Y.G. Kim, H.T. Kim, and Y.C. Jeun. 2010. Controlling activity of Bacillus subtilis KB-401 against cucumber powdery mildew caused by Sphaerotheca fusca. Kor. J. Pesticide Sci. 14(1):49-53 (in Korean).
  11. Remigio, A.G.P., R.M. Davis, and J.J. Marois. 2003. Effects of relative humidity and high temperature on spore germination and development of tomato powdery mildew (Leveillula taurica). Crop Protection 22(10):1157-1168. https://doi.org/10.1016/S0261-2194(03)00157-1
  12. Shin, H.D. 2000. Erysiphaceae of Korea. National Institute of Agricultural Science and Technology, Suwon, Korea. p. 227-235.
  13. Sundheim, L. and J. Amunden. 1982. Fungicide tolerance in the hyperparasite Ampelomyces quisqualis and integrated control of cucumber powdery mildew. Acta Agri. Scand. 32:349-355. https://doi.org/10.1080/00015128209435331
  14. Sztejnberg, A. and S. Mazar. 1985. Biocontrol of cucumber and carrot powdery mildew by Ampelomyces quisqualis. Phytopathology 75:1301-1302.
  15. Zitter, T.A., D. Hopkins, and C.E. Thomas. 1996. Compendium of Cucurbit Diseases. APS Press, ST. Paul, MN, USA. p. 87.