Biomedical Science Letters (대한의생명과학회지)

The Korean Society for Biomedical Laboratory Sciences (대한의생명과학회)
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Polymorphisms of Cytochrome P450 2E1 Gene in Korean Patients with Renal Failure

  • Yoo, Min (Department of Biology, Keimyung University)
  • Received : 2013.08.30
  • Accepted : 2013.11.08
  • Published : 2013.12.31
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Abstract

CYP2E1 in the liver has been studied intensively because it is involved in the metabolic activation of xenobiotics. It is inducible by alcohol, so it has been suspected as the cause of cancer in the stomach and lung. The possible role of CYP2E1 has been suggested strongly as causing tissue damage in mice with renal failure. It was also suspected that 5'-flanking region of CYP2E1 gene might be involved with renal failure. So, we investigated polymorphism of restriction enzyme sites within CYP2E1 gene using the PCR-RFLP analysis. PstI and RsaI sites were located at 5'-flanking region and DraI site was located at intron 6. All three types (W/W, W/S, S/S) were observed for these enzymes although each incidence was somewhat different depending the enzyme sites. W/W was prominent for PstI whereas W/S was markedly high for RsaI. Overall, polymorphic incidence in patients was somewhat higher than normal population. This research should facilitate further investigation of CYP2E1 at genetic level as the direct cause of tissue damage in various organs.

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References

  1. Ahn EJ, Lee RA, Chung SS, Kim KH, Park EB. The colorectal cancer risk of meat intake, smoking, and CYP2E1 polymorphisms: The comparison of colorectal cancer patients with controls. J Korean Surg Soc. 2006. 71: 262-268.
  2. Cai L, Zheng ZL, Zhang ZF. Cytochrome P450 2E1 polymorphism and the risk of gastric cancer. Toxicol. 1998. 152: 276-281.
  3. Das J, Ghosh J, Manna P, Parames CS. Taurine protects acetaminophen-induced oxidative damage in mice kidney through APAP urinary excretion and CYP2E1 inactivation. Toxicol. 2010. 269: 24-34. https://doi.org/10.1016/j.tox.2010.01.003
  4. Fritsche E, Pettman GS, Bell DA. Localization, sequence analysis and ethnic distribution of a 96 bp insertion in the promoter of the human CYP2E1 gene. Mutat Res Genomics. 2000. 432: 1-5. https://doi.org/10.1016/S1383-5726(99)00009-6
  5. Guengerich FP, Kim DH, Iwasaki M. Role of human cytochrome P450 2E1 in the oxidation of many low molecular weight cancer suspects.Chem Res Toxicol. 1991. 4: 168-179. https://doi.org/10.1021/tx00020a008
  6. Hayashi SI, Watanabe J, Kawajiri K. Genetic polymorphisms in the 5'-flanking region change transcriptional regulation of the human cytochrome P450 2E1 gene. J Biochem. 1991. 110: 559-565. https://doi.org/10.1093/oxfordjournals.jbchem.a123619
  7. Kato S, Shieds PG, Caporaso NE. Cytochrome P450 2E1 genetic polymorphism, racial variation, and lung cancer risk. Cancer Res. 1992. 52: 6712-6715.
  8. Kim RB, O'Shea D, Wilkinson GR. Interindividual variability of chlorzoxazone 6-hydroxylation in men and women and its relationship to CYP2E1 genetic polymorphisms. Clin Pharmacol Ther. 1995. 57: 645-655. https://doi.org/10.1016/0009-9236(95)90227-9
  9. Nam HM, Park JW, Song YJ, Yun HY, Park JS, Hyun TS. Kimchi and soybean pastes are risk factors of gastric cancer. World J Gastroenterol. 2005. 11: 3175-3181. https://doi.org/10.3748/wjg.v11.i21.3175
  10. McCarver DG, Byun R, Hines RN, Hichme M, Wegenek W. A genetic polymorphism in the regulatory sequences of human CYP2E1: Association with increased chlorzoxazone hydroxylation in the presence of obesity and ethanol intake. Toxicol Appl Pharmacol. 1998. 152: 276-281. https://doi.org/10.1006/taap.1998.8532
  11. Ohkubo Y, Ueta A, Ando N, Ito T, Yamaguchi S, Mizuno K. Novel mutations in the cytochrome P450 2C19 gene: a pitfall of the PCR-RFLP method for identifying a common mutation. J Hum Genet. 2006. 51: 118-123. https://doi.org/10.1007/s10038-005-0332-y
  12. Paik SW, Yoon JH, Hwang YJ, Jang YH, Lee HS, Kim CY. Genetic Polymorphism of Cytochrome P450 2E1 in Hepatocellular Carcinoma Patients in Korea. Korean J Gastroenterol. 30: 503-512.
  13. Park JS, Kim H, Song YJ, Yun HY, Kang JW, Kim YD, Nan HM. Effects of environmental carcinogens and genetic polymorphisms of CYP2E1 and NAT2 on gastric carcinogenesis. J Korean Surg Soc. 2000. 59: 488-499.
  14. Seitz HK, Simanowsky UA. Alcohol and carcinogenesis. Ann Rev Nutr. 1988. 8: 99-119. https://doi.org/10.1146/annurev.nu.08.070188.000531
  15. Tsutsumi M, Matsuda Y, Takada A. Role of ethanol inducible cytochrome P450 2E1 in the development of hepatocellular carcinoma by the chemical carcinogen, N-nitrosodimethylamine. Hepatology. 1993. 18: 1483-1489. https://doi.org/10.1002/hep.1840180630
  16. Uematsu F, Kikuchi H, Motomiya M. Association between restriction fragment length polymorphism of the human cytochrome P450 2E1 in a Swedish population: relationship to incidence of lung cancer. Febs Lett. 1993. 319: 207-211. https://doi.org/10.1016/0014-5793(93)80547-8