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The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Ethanol Extract of Astragalus membranaceus Bunge Induces Aquaporin-3 Expression in HaCaT Cells

HaCaT Cell에서 황기 에탄올 추출물의 Aquaporin-3 발현 효과

  • Park, Hyun-Chul (Saimdang Cosmetics Co., Ltd., R&D Center) ;
  • Kim, Hee-Taek (Dept. of Oriental Medical Opthalmology & Otolaryngology & Dermatology, Semyung University Oriental Medical Hospital) ;
  • Ha, Hun-Young (Dept. of Natural Medicine Resources, Semyung University) ;
  • Lee, Pyeong-Jae (Dept. of Natural Medicine Resources, Semyung University) ;
  • Yoon, Kyung-Sup (Saimdang Cosmetics Co., Ltd., R&D Center)
  • 박현철 ((주)사임당화장품 기술연구소) ;
  • 김희택 (세명대학교 한의과대학 안이비인후피부과학교실) ;
  • 하헌용 (세명대학교 자연약재과학과) ;
  • 이평재 (세명대학교 자연약재과학과) ;
  • 윤경섭 ((주)사임당화장품 기술연구소)
  • Received : 2013.08.07
  • Accepted : 2013.11.11
  • Published : 2013.12.30
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Abstract

Astragalus membranaceus Bunge is used in herbal medicine in Eastern Asian countries including Korea. In this study, we assessed the effects of A. membranaceus extract (AM) on the aquaporin-3 (AQP3) protein expression in HaCaT cells. AM did not affect viability of HaCaT cells. AQP3 expression and cell migration seem to be maximal at $100{\mu}g/mL$ concentration. Epidermal growth factor receptor (EGFR) kinase inhibitor, PD153035, blocked AM-induced AQP3 expression and cell migration. In addition, an 80% ethanol extracts of herbal prescription, SinhyoTakleesan (ST), which is composed of A. membranaceus, Angelicae gigantis, Glycyrrhiza glabra Linne, and Lonicera japonica Flos also induced AQP3 expression at $20{\mu}g/mL$ in HaCaT cells. Collectively, these results suggest that AM induce AQP3 expression via EGFR pathway.

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References

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