Korean Journal of Agricultural Science (농업과학연구)

Institute of Agricultural Science, CNU (충남대학교 농업과학연구소)
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Diversity of Calpain-Calpastatin gene frequencies in Brown, Brindle and Jeju Black Hanwoo

한우, 칡소 및 제주 흑우 Calpain-Calpastatin 유전자 다양성

  • 이승환 (국립축산과학원 한우시험장) ;
  • 김승창 (국립축산과학원 동물 유전체과) ;
  • 조수현 (국립축산과학원 축산물이용과) ;
  • 최봉환 (국립축산과학원 동물 유전체과) ;
  • ;
  • 임다정 (국립축산과학원 한우시험장) ;
  • 당창권 (국립축산과학원 한우시험장) ;
  • 장선식 (국립축산과학원 한우시험장) ;
  • 김재환 (국립축산과학원 유전자원시험장) ;
  • 고문석 (국립축산과학원 난지축산시험장) ;
  • 양보석 (국립축산과학원 한우시험장) ;
  • 강희설 (국립축산과학원 한우시험장)
  • Received : 2013.05.13
  • Accepted : 2013.06.12
  • Published : 2013.06.30
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Abstract

The aim of study was to investigate genetic diversity for the calpain/calpastatin gene in three Hanwoo breeds [(Brown (n=62), Brindle (n=81) and Jeju Black (n=30)]. Random samples from three breeds of Hanwoo were selected and genotyped for the 7 SNPs of calpain/calpastatin using TaqMan method. Allele frequencies were investigated for CAPN1/CAST gene. Allele frequency of CAST2 SNP was 0.75, 0.59 and 0.22 for Brown, Brindle and Jeju black, respectively. The CAST3 revealed allele frequency of 0.59 and 0.57 in Brown and Jeju Black, while it showed very low allele frequency (0.07) in Brindle. In particular, favorable allele (G allele) for the CAPN1-2 SNP which was shown a strong association with tenderness in Taurine and Indicine cattle revealed 16% and 17% higher allele frequency in Brown Hanwoo (0.82) comparing Brindle (0.66) and Jeju Black Hanwoo (0.65). AMOVA demonstrated that among population variance occupied only 10% of total variance and among individual variance was 0%, while within individual variance was 90% of total variance. This result showed that population effect contributed very small portion of genetic to these three Hanwoo breeds, while within individual variance contributed large portion of genetic diversity within these Hanwoo breeds. In conclusion, three Hanwoo breeds (Brown, Brindle and Jeju black) showed a genetically homogeneous based on the 7 SNPs of CAPN1/CAST gene and it came from same ancestor to form modern Hanwoo breed.

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References

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