Journal of Animal Environmental Science (한국축산시설환경학회지)

The Korean Society of Animal Environmental Science & Technology (한국축산환경학회)
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In vitro Technique for Selection of Radiation Induced Mutants of Tall Fescue

방사선 처리에 의한 톨 페스큐 돌연변이 식물체 선발

  • Lee, Ki-Won (Grassland & Forages Division, National Institute of Animal Science, RDA) ;
  • Moon, Jin Young (Grassland & Forages Division, National Institute of Animal Science, RDA) ;
  • Ji, Hee Chung (Grassland & Forages Division, National Institute of Animal Science, RDA) ;
  • Choi, Gi Jun (Grassland & Forages Division, National Institute of Animal Science, RDA) ;
  • Kim, Ki-Yong (Grassland & Forages Division, National Institute of Animal Science, RDA) ;
  • Hwang, Tae Young (Grassland & Forages Division, National Institute of Animal Science, RDA) ;
  • Lee, Sang-Hoon (Grassland & Forages Division, National Institute of Animal Science, RDA)
  • 이기원 (농촌진흥청 국립축산과학원 초지사료과) ;
  • 문진용 (농촌진흥청 국립축산과학원 초지사료과) ;
  • 지희정 (농촌진흥청 국립축산과학원 초지사료과) ;
  • 최기준 (농촌진흥청 국립축산과학원 초지사료과) ;
  • 김기용 (농촌진흥청 국립축산과학원 초지사료과) ;
  • 황태영 (농촌진흥청 국립축산과학원 초지사료과) ;
  • 이상훈 (농촌진흥청 국립축산과학원 초지사료과)
  • Received : 2013.08.25
  • Accepted : 2013.09.22
  • Published : 2013.09.30
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Abstract

In vitro culture and radiation techniques were used for obtaining mutants tin tall fescue. Endophyte free and friendly tall fescue cultivars Kentucky-31 and Jesup were used for induction of genetic variability through in-vitro mutagenesis. Mature seeds was used for callus induction on 6 mg/L 2,4-D. Actively growing and compact callus was treated with three different doses of gamma rays (10 Gy, 30 Gy and 50 Gy). Maximum proliferation and plantlets regeneration growth was observed in control and minimum at 10 Gy. Furthermore, the maximum number of tiller in the irradiated population was observed in 10 Gy. The treatments 30 Gy and 50 Gy exhibited negative impact on the tillering potential of the tall fescue plant. The object of this study was to develop protocols for mutation breeding in tall fescue through radiation techniques.

Keywords

References

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