Journal of Animal Environmental Science (한국축산시설환경학회지)

The Korean Society of Animal Environmental Science & Technology (한국축산환경학회)
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Callus Induction and Plant Regeneration Efficiency According to Tissue Culture Conditions in Teff grass (Eragrostis)

테프그라스 조직배양을 통한 캘러스 형성 및 식물체 재분화 효율

  • Lee, Ki-Won (Grassland & Forages Division, National Institute of Animal Science, Rural Development Administration) ;
  • Moon, Jin Young (Grassland & Forages Division, National Institute of Animal Science, Rural Development Administration) ;
  • Park, Hyung Soo (Grassland & Forages Division, National Institute of Animal Science, Rural Development Administration) ;
  • Choi, Gi Jun (Grassland & Forages Division, National Institute of Animal Science, Rural Development Administration) ;
  • Kim, Ki-Yong (Grassland & Forages Division, National Institute of Animal Science, Rural Development Administration) ;
  • Ji, Hee Chung (Grassland & Forages Division, National Institute of Animal Science, Rural Development Administration) ;
  • Hwang, Tae Young (Grassland & Forages Division, National Institute of Animal Science, Rural Development Administration) ;
  • Lee, Sang-Hoon (Grassland & Forages Division, National Institute of Animal Science, Rural Development Administration)
  • 이기원 (농촌진흥청 국립축산과학원 초지사료과) ;
  • 문진용 (농촌진흥청 국립축산과학원 초지사료과) ;
  • 박형수 (농촌진흥청 국립축산과학원 초지사료과) ;
  • 최기준 (농촌진흥청 국립축산과학원 초지사료과) ;
  • 김기용 (농촌진흥청 국립축산과학원 초지사료과) ;
  • 지희정 (농촌진흥청 국립축산과학원 초지사료과) ;
  • 황태영 (농촌진흥청 국립축산과학원 초지사료과) ;
  • 이상훈 (농촌진흥청 국립축산과학원 초지사료과)
  • Received : 2013.08.25
  • Accepted : 2013.09.25
  • Published : 2013.09.30
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Abstract

Teff grass is a warm season C4 annual grass that is used for dry hay, silage and haylage. We have developed a high-frequency plant regeneration system for teff grass via callus culture using mature seeds. It was revealed that mature seeds cultured on MS medium supplemented with 2 mg/l 2,4-D, 0.5 g/L proline, 0.5 g/L casamino acid and 3 g/L Gelrite under light condition produced the highest percentage of callus formation (91.9%). Addition of cytokinins (BA) at 0.0~0.5 mg/L to media containing 2 mg/l 2,4-D enhanced callus growth. The most suitable medium for plant regeneration from dehydrated calli was MS agar medium supplemented with 0.1 mg/l NAA, 1 mg/l BA, 0.5 g/L proline, 0.5 g/L casamino acid 3 g/L Gelrite which induced the highest percentage of calli forming shoots (47.0%). The shoots were rooted at the highest rate (100%) when transferred onto 1/2 MS medium and acclimated in greenhouse conditions.

Keywords

References

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