Asian Pacific Journal of Cancer Prevention

Asian Pacific Journal of Cancer Prevention (아시아태평양암예방학회)
권호 표지
DOI QR코드

DOI QR Code

Experimental Study of Endostar Injection Concomitant with Cryoablation on Lung Adenocarcinoma A549 Xenografts

  • Ma, Chun-Hua (Department of Intervention, Tianjin Huanhu Hospital, Tianjin Cerebral Vascular and Neural Degenerative Disease Key Laboratory) ;
  • Jiang, Rong (Department of Intervention, Tianjin Huanhu Hospital, Tianjin Cerebral Vascular and Neural Degenerative Disease Key Laboratory) ;
  • Li, Jin-Duo (Department of Intervention, Tianjin Huanhu Hospital, Tianjin Cerebral Vascular and Neural Degenerative Disease Key Laboratory) ;
  • Wang, Bin (Department of Intervention, Tianjin Huanhu Hospital, Tianjin Cerebral Vascular and Neural Degenerative Disease Key Laboratory) ;
  • Sun, Li-Wei (Department of Intervention, Tianjin Huanhu Hospital, Tianjin Cerebral Vascular and Neural Degenerative Disease Key Laboratory) ;
  • Lv, Yuan (Department of Intervention, Tianjin Huanhu Hospital, Tianjin Cerebral Vascular and Neural Degenerative Disease Key Laboratory)
  • Published : 2013.11.30
Download PDF
⟨ Previous Next ⟩

Abstract

Objective: To explore the inhibiting effect and mechanism of Endostar injection concomitant with cryoablation on lung adenocarcinoma A549 xenografts in nude mice. Materials and Methods: A total of 24 nude mice with subcutaneous xenografts of the A549 cell line were established and divided into 4 groups when the maximal diameters of tumors became 1 cm: control group, Endostar group, cryoablation group and combination group (Endostar concomitant with cryoablation). The nude mice were sacrificed after 21-days treatment, tumour tissues were removed to measure their volume, in situ test of TdT-mediated dUTP nick end labeling (TUNEL) was adopted to determine the cellular apoptosis around freezing injury zones, and immunohistochemical SP test was applied for the detection of micro-vessel density (MVD) and vascular endothelial growth factor (VEGF) expression levels. Results: At 21-days after treatment, the growth velocities of control group, Endostar group, cryoablation group and combination group were $236.7{\pm}51.2%$, $220.0{\pm}30.6%$, $159.5{\pm}29.3%$ and $103.3{\pm}25.5%$ (P<0.01), while cellular apoptosis rates of tumors were $21.7{\pm}2.34%$, ($22.17{\pm}1.47$)%, $38.3{\pm}1.37%$ and $49.2{\pm}1.72%$, (P<0.01), respectively, according to the immunohistochemical test. MVD and VEGF expression levels in the combination group were both lower than in other groups (P<0.01), also being positively related (r=0.925, P<0.01). Conclusions: Endostar can significantly improve the inhibitory effects of cryoablation on xenografts of lung adenocarcinoma A549, and the mechanism is probably associated with its function as an inhibitor of tumour neo-angiogenesis through down-regulating VEGF expression.

Keywords

References

  1. Fei ZH, Yao CY, Yang XL, et al (2013). Serum BMP-2 Up-regulation as an Indicator of Poor Survival in Advanced Non-small Cell Lung Cancer Patients. Asian Pac J Cancer Prev, 14, 5293-9. https://doi.org/10.7314/APJCP.2013.14.9.5293
  2. Folkman J (2006). Antiangiogenesis in cancer therapy-endostatin and its mechamisms of action. Exp Cell Res, 312, 594-607. https://doi.org/10.1016/j.yexcr.2005.11.015
  3. Gary Onik (2008). Rationale for a "Male Lumpectomy" a Prostate Cancer targeted approach using cryoablation: results in 21 patients with at Least 2 years of follow-up. Cardiovasc Intervent Radiol, 31, 98-106. https://doi.org/10.1007/s00270-004-0232-0
  4. Guo Z, Wang HT, Xing WG, et al (2010). A preliminary clinical study of targeted cryoablation of prostate in the treatment of T3N0M0 prostate cancer. National Med J Chin, 90, 2815-9.
  5. Hu KW, Li QW, Zuo MH, et al (2007). Clinical observation on the combined treatment of 57 cases of non-small cell lung cancer using argon-helium cryosurgery and Chinese herbal medicine. Chin J Integr Med, 13, 224-7. https://doi.org/10.1007/s11655-007-0224-4
  6. Kimura M, Rabbani Z, Mouraviev V, et al (2010). Morphology of hypoxia following cryoablation in a prostate cancer murine model: its relationship to necrosis, apoptosis and, microvessel density. Cryobiology, 61, 148-54. https://doi.org/10.1016/j.cryobiol.2010.06.010
  7. Lee CG, Heijn M, di Tomaso E, et al (2000). Anti-vascular endothelial growth factor treatment augments tumor radiation response under normoxic or hypoxic conditions. Cancer Res, 60, 5565-70.
  8. Liu YC, Zhou SB, Gao F, et al (2013).Chemotherapy and late course three dimensional conformal radiotherapy for treatment of patients with stage III non- small cell lung cancer. Asian Pac J Cancer Prev, 14, 2663-5. https://doi.org/10.7314/APJCP.2013.14.4.2663
  9. Lu YY, Huang XE, Xu L, et al (2013). Potential predictors of sensitivity to pemetrexed as first-line chemotherapy for patients with advanced non-squamous NSCLCs. Asian Pac J Cancer Prev, 14, 2005-8. https://doi.org/10.7314/APJCP.2013.14.3.2005
  10. Mauceri HJ, Hanna NN, Beckett MA, et al (1998). Combined effects of angiostatin and ionizing radiation in antitumourtherapy. Nature, 394, 287- 91. https://doi.org/10.1038/28412
  11. Ueda M,Terai Y,Kurnagai K, et a1 (1999).Correlation between tumor angiogenesis and expression of thymidine phosphorylase, and patient outcome in uterine cervical carcinoma. Hum Pathol, 30, 1389-94. https://doi.org/10.1016/S0046-8177(99)90074-6
  12. Wang JW, Sun Y, Liu YY, et al (2005). A random, double-blind, control, multi-center phase III clinical study of Endostatin concomitant with NP protocol on advanced NSCLC. Chin J Lung Cancer, 48, 283-90.
  13. Xing WG, Guo Z, Wang HT, et al (2008). Percutaneous cryoablation of prostate cancer guided by rectal ultrasound: a retrospective analysis of 42 cases. Chin J Radio, 42, 807-11.
  14. Yan HA, Shen K, Huang XE (2013). Clinical study on mannan peptide combined with TP regimen in treating patients with non-small cell lung cancer. Asian Pac J Cancer Prev, 14, 4801-4. https://doi.org/10.7314/APJCP.2013.14.8.4801
  15. Yin HT, Tian QZ, Guan L (2013). In vitro and in vivo evaluation of the antitumor efficiency of resveratrol against lung cancer. Asian Pac J Cancer Prev, 14, 1703-6. https://doi.org/10.7314/APJCP.2013.14.3.1703

Cited by

  1. Bevacizumab Concomitant with Chemotherapy is Effective in Treating Chinese Patients with Advanced Non-Squamous Non-Small Cell Lung Cancer vol.15, pp.14, 2014, https://doi.org/10.7314/APJCP.2014.15.14.5945
  2. Expression of Inhibitor of Differentiation-1 and Its Effects on Angiogenesis in Gastric Cancer vol.31, pp.7, 2016, https://doi.org/10.1089/cbr.2016.2043
  3. Titanium dioxide nanofibers induce angiogenic markers and genomic instability in lung cells leading to a highly dedifferentiated and fibrotic tumor formation in a xenograft model vol.6, pp.1, 2019, https://doi.org/10.1039/C8EN01078A