Asian Pacific Journal of Cancer Prevention

Asian Pacific Journal of Cancer Prevention (아시아태평양암예방학회)
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Kupffer Cells Suppress Hepatocarcinogenesis and Metastasis in Tumor Orthotopic Implanted Kunming Mice

  • Li, X.Y. (Department of Hepatobiliary surgery, Traditonal Chinese Medicine Hospital of Dianjiang) ;
  • Wang, M.Y. (Department of Hepatobiliary surgery, The Second Affiliated Hospital of Chongqing Medical University) ;
  • Zhang, J.Y. (Department of Hepatobiliary surgery, The Second Affiliated Hospital of Chongqing Medical University) ;
  • Li, J.Z. (Department of Hepatobiliary surgery, The Second Affiliated Hospital of Chongqing Medical University) ;
  • Gong, J.P. (Department of Hepatobiliary surgery, The Second Affiliated Hospital of Chongqing Medical University) ;
  • Zhang, Wei (Department of Hepatobiliary surgery, The Second Affiliated Hospital of Chongqing Medical University)
  • Published : 2013.11.30
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Abstract

In this research, we used $GdCl_3$ (gadolinium chloride) to restrain the function of Kupffer cells and assessed effects on hepatocarcinogenesis and metastasis in the Kunming mouse. A 0.25% $GdCl_3$ solution (10 mg/kg b.w.) was infused via the vena caudalis of each mouse 1 week before inoculation of H22 cells and was continued once per three days. Then we observed the follow indexes 3 weeks after injection of H22 cells: tumor weight, histologic characteristics of tumor tissue by light microscopy, ultramicrostructure of Kupffer cells under the electron microscope, distribution and number of Kupffer cells by histochemical staining, and TNF-${\alpha}$ and IFN-${\gamma}$ levels in blood-serum and liver tissue by ELISA and RT-PCR. MMP-2 protein expression was tested by immunohistochemistry. The $GdCl_3$ pretreatment had no effect on the quantity of Kupffer cells, but clearly restrained their functions, with decrease of TNF-${\alpha}$ and IFN-${\gamma}$ levels and elevation of MMP2. Tumor immunity functions were markedly suppressed and tumor growth was accelerated with appearance of metastasis. Furthermore, survival time of trial mice was shortened.

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References

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