Asian Pacific Journal of Cancer Prevention

  • 제14권10호
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  • Pages.6089-6094
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  • 2013
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  • 1513-7368(pISSN)
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  • 2476-762X(eISSN)
아시아태평양암예방학회 (Asian Pacific Journal of Cancer Prevention)
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Genomic Screening for Targets Regulated by Berberine in Breast Cancer Cells

  • Wen, Chun-Jie (Institute of Life Sciences, Chongqing Medical University) ;
  • Wu, Lan-Xiang (Institute of Life Sciences, Chongqing Medical University) ;
  • Fu, Li-Juan (Institute of Life Sciences, Chongqing Medical University) ;
  • Yu, Jing (Institute of Clinical Pharmacology, Central South University) ;
  • Zhang, Yi-Wen (Institute of Clinical Pharmacology, Central South University) ;
  • Zhang, Xue (Institute of Life Sciences, Chongqing Medical University) ;
  • Zhou, Hong-Hao (Institute of Life Sciences, Chongqing Medical University)
  • 발행 : 2013.10.30
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초록

Berberine, a common isoquinoline alkaloid, has been shown to possess anti-cancer activities. However, the underlying molecular mechanisms are still not completely understood. In the current study, we investigated the effects of berberine on cell growth, colony formation, cell cycle distribution, and whether it improved the anticancer efficiency of cisplatin and doxorubicin in human breast cancer estrogen receptor positive (ER+) MCF-7 cells and estrogen receptor negative (ER-) MDA-MB-231 cells. Notably, berberine treatment significantly inhibited cell growth and colony formation in the two cell lines, berberine in combination with cisplatin exerting synergistic growth inhibitory effects. Accompanied by decreased growth, berberine induced G1 phase arrest in MCF-7 but not MDA-MB-231 cells. To provide a more detailed understanding of the mechanisms of action of berberine, we performed genome-wide expression profiling of berberine-treated cells using cDNA microarrays. This revealed that there were 3,397 and 2,706 genes regulated by berberine in MCF-7 and MDA-MB-231 cells, respectively. Fene oncology (GO) analysis identified that many of the target genes were involved in regulation of the cell cycle, cell migration, apoptosis, and drug responses. To confirm the microarray data, qPCR analysis was conducted for 10 selected genes based on previously reported associations with breast cancer and GO analysis. In conclusion, berberine exhibits inhibitory effects on breast cancer cells proliferation, which is likely mediated by alteration of gene expression profiles.

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참고문헌

  1. Ahonen M, Ala-Aho R, Baker AH, et al (2002). Antitumor activity and bystander effect of adenovirally delivered tissue inhibitor of metalloproteinases-3. Mol Ther, 5, 705-15. https://doi.org/10.1006/mthe.2002.0606
  2. Balkwill F (2004). The significance of cancer cell expression of the chemokine receptor CXCR4. Semin Cancer Biol, 14, 171-9. https://doi.org/10.1016/j.semcancer.2003.10.003
  3. Chen L, Chen J, Xu H (2013). Sasanquasaponin from Camellia oleifera Abel. induces cell cycle arrest and apoptosis in human breast cancer MCF-7 cells. Fitoterapia, 84, 123-9. https://doi.org/10.1016/j.fitote.2012.11.009
  4. Imanshahidi M, Hosseinzadeh H (2008). Pharmacological and therapeutic effects of Berberis vulgaris and its active constituent, berberine. Phytother Res, 22, 999-1012. https://doi.org/10.1002/ptr.2399
  5. Jemal A, Bray F, Center MM, et al (2011). Global cancer statistics. CA Cancer J Clin, 61, 69-90. https://doi.org/10.3322/caac.20107
  6. Kim JB, Lee KM, Ko E, et al (2008a). Berberine inhibits growth of the breast cancer cell lines MCF-7 and MDA-MB-231. Plant Med, 74, 39-42. https://doi.org/10.1055/s-2007-993779
  7. Kim S, Choi JH, Kim JB, et al (2008b). Berberine suppresses TNF-alpha-induced MMP-9 and cell invasion through inhibition of AP-1 activity in MDA-MB-231 human breast cancer cells. Molecules, 13, 2975-85. https://doi.org/10.3390/molecules13122975
  8. Kim S, Han J, Lee SK, et al (2012). Berberine suppresses the TPA-induced MMP-1 and MMP-9 expressions through the inhibition of PKC-${\alpha}$ in breast cancer cells. J Surq Res, 176e, 21-9.
  9. Koren R, Rocker D, Kotestiano O, Liberman UA, Ravid A (2000). Synergistic anticancer activity of 1,25-dihydroxyvitamin D3 and immune cytokines: the involvement of reactive oxygen species. J Steroid Biochem Mol Biol, 73, 105-12. https://doi.org/10.1016/S0960-0760(00)00068-6
  10. Kuo CL, Chi CW, Liu TY (2005). Modulation of apoptosis by berberine through inhibition of cyclooxygenase-2 and Mcl-1 expression in oral cancer cells. In Vivo, 19, 247-52.
  11. Lee YS, Kim WS, Kim KH, et al (2006). Berberine, a natural plant product, activates AMP-activated protein kinase with beneficial metabolic effects in diabetic and insulin-resistant states. Diabetes, 55, 2256-64. https://doi.org/10.2337/db06-0006
  12. Liu J, He CW, Zhou KY, Wang JD, Kang JX (2009). Coptis extracts enhance the anticancer effect of estrogen receptor antagonists on human breast cancer cells. Biochem Biophys Res Commun, 378, 174-8. https://doi.org/10.1016/j.bbrc.2008.10.169
  13. Livak KJ, Schmittgen TD (2001). Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T).). Method. Methods, 25, 402-8. https://doi.org/10.1006/meth.2001.1262
  14. Meeran SM, Katiyar S, Katiyar SK (2008). Berberine-induced apoptosis in human prostate cancer cells is initiated by reactive oxygen species generation. Toxicol Appl Pharmacol, 229, 33-43. https://doi.org/10.1016/j.taap.2007.12.027
  15. Okuno S, Sato H, Kuriyama-Matsumura K, et al (2003). Role of cystine transport in intracellular glutathione level and cisplatin resistance in human ovarian cancer cell lines. Br J Cancer, 88, 951-6. https://doi.org/10.1038/sj.bjc.6600786
  16. Patil JB, Kim J, Jayaprakasha GK (2010). Berberine induces apoptosis in breast cancer cells (MCF-7). through mitochondrial-dependent pathway. Eur J Pharmacol, 645, 70-8. https://doi.org/10.1016/j.ejphar.2010.07.037
  17. Sekhon BK, Roubin RH, Tan A, Chan WK, Sze DM (2008). High-throughput screening platform for anticancer therapeutic drug cytotoxicity. Assay Drug Dev Technol, 6, 711-21. https://doi.org/10.1089/adt.2008.148
  18. Singh T, Vaid M, Katiyar N, Sharma S, Katiyar SK (2011). Berberine, an isoquinoline alkaloid, inhibits melanoma cancer cell migration by reducing the expressions of cyclooxygenase-2, prostaglandin E2 and prostaglandin E2 receptors. Carcinogenesis, 32, 86-92. https://doi.org/10.1093/carcin/bgq215
  19. Tan MJ, Teo ZQ, Sng MK, Zhu PC, Tan NS (2012). Emerging roles of angiopoietin-like 4 in human cancer. Mol Cancer Res, 10, 677-88. https://doi.org/10.1158/1541-7786.MCR-11-0519
  20. Tan YL, Goh D, Ong ES (2006). Investigation of differentially expressed proteins due to the inhibitory effects of berberine in human liver cancer cell line HepG2. Mol Biosyst, 2, 250-8. https://doi.org/10.1039/b517116d
  21. Tillhon M, Guaman Ortiz LM, Lombardi P, Scovassi AI (2012). Berberine: New perspectives for old remedies. Biochemical Pharmacology, 84, 1260-7. https://doi.org/10.1016/j.bcp.2012.07.018
  22. Tsuchiya Y, Nakajima M, Yokoi T (2005). Cytochrome P450-mediated metabolism of estrogens and its regulation in human. Cancer Lett, 227, 115-24. https://doi.org/10.1016/j.canlet.2004.10.007
  23. Wang W, Huper G, Guo Y, et al (2005). Analysis of methylation-sensitive transcriptome identifies GADD45a as a frequently methylated gene in breast cancer. Oncogene, 24, 2705-14. https://doi.org/10.1038/sj.onc.1208464
  24. Wu K, Yang Q, Mu Y, et al (2011). Berberine inhibits the proliferation of colon cancer cells by inactivating Wnt/${\beta}$-catenin signaling. Int J Oncol, 41, 292-8.

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  2. A gene expression signature-based approach reveals the mechanisms of action of the Chinese herbal medicine berberine vol.4, pp.1, 2015, https://doi.org/10.1038/srep06394
  3. Caractérisation des alcaloïdes isoquinoléique de Fumaria agraria et évaluation de leur potentiel antiprolifératif vis-à-vis des cellules cancéreuses mammaires vol.14, pp.3, 2016, https://doi.org/10.1007/s10298-015-0981-8
  4. Berberine enhances the anti-tumor activity of tamoxifen in drug-sensitive MCF-7 and drug-resistant MCF-7/TAM cells vol.14, pp.3, 2016, https://doi.org/10.3892/mmr.2016.5490
  5. The antioxidant and chemical properties of Berberis vulgaris and its cytotoxic effect on human breast carcinoma cells vol.68, pp.4, 2016, https://doi.org/10.1007/s10616-015-9880-y