대한의용생체공학회:의공학회지 (Journal of Biomedical Engineering Research)

  • 제34권4호
  • /
  • Pages.189-196
  • /
  • 2013
  • /
  • 1229-0807(pISSN)
  • /
  • 2288-9396(eISSN)
대한의용생체공학회 (The Korean Society of Medical and Biological Engineering)
권호 표지
DOI QR코드

DOI QR Code

미세유체칩내 electrode의 opening window형태에 따른 유전전기영동력 특성 규명

Characterization of Dielectrophoretic Force for the Structural Shapes of Window in Microfluidic Dielectrophoretic Chip

  • Lee, Jaewoo (Department of Biomedical Engineering, Yonsei University) ;
  • Kwak, Tae Joon (Department of Biomedical Engineering, Yonsei University) ;
  • Yoon, Dae Sung (Department of Biomedical Engineering, Yonsei University) ;
  • Lee, Sang Woo (Department of Biomedical Engineering, Yonsei University)
  • 투고 : 2013.08.27
  • 심사 : 2013.11.26
  • 발행 : 2013.12.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Dielectrophoresis(DEP) is useful in manipulation and separation of micro-sized particles including biological samples such as bacteria, blood cells, and cancer cells in a micro-fluidic device. Especially, those separation and manipulation techniques using DEP in combination of micro fabrication technique have been researched more and more. Recently, it is revealed that a window structure of insulating layer in microfluidic DEP chip is key role in trap of micro-particles around the window structure. However, the trap phenomenon-driven by DEP force gradient did not fully understand and is still illusive. In this study, we characterize the trap mechanism and efficiency with different shapes of window in a microfluidic DEP chip. To do this characterization, we fabricated 4 different windows shapes such as rhombus, circle, squares, and hexagon inside a micro-fluidic chip, and performed micro-sized particles manipulation experiments as varying the frequency and voltage of AC signal. Moreover, the numerical simulation with the same parameters that were used in the experiment was also performed in order to compare the simulation results and the experimental results. Those comparison shows that both results are closely matched. This study may be helpful in design and development of microfluidic DEP chip for trapping micro-scaled biological particle.

키워드

참고문헌

  1. S.H. Chung, B.J. Choi, S.W. Lee, D.S. Yoon. "Recent research trends in nanoscale electro-mechanical systems for bio-medical applications" Biomedical Engineering Letters, vol. 1, Issue 1, pp. 7-10, 2011. https://doi.org/10.1007/s13534-011-0009-8
  2. D'souza, S. F. "Microbial biosensors." Biosensors and Bioelectronics, vol. 16, pp. 337-353, 2001. https://doi.org/10.1016/S0956-5663(01)00125-7
  3. Satake, D., H. Ebi, et al. "A sensor for blood cell counter using MEMS technology." Sensors and Actuators B, vol. 83, pp. 77-81, 2002. https://doi.org/10.1016/S0925-4005(01)01045-0
  4. Shafiee, Hadi, et al. "Selective isolation of live/dead cells using contactless dielectrophoresis (cDEP)." Lab on a Chip, vol. 10, pp. 438-445, 2010. https://doi.org/10.1039/b920590j
  5. Pohl, Herbert A. "The motion and precipitation of suspensoids in divergent electric fields." Journal of Applied Physics, vol. 22, pp. 869-871, 1951. https://doi.org/10.1063/1.1700065
  6. S.H. Baek, et al. "Dielectrophoretic technique for measurement of chemical and biological interactions." Analytical Chemistry, vol. 81, pp. 7737-7742, 2009 https://doi.org/10.1021/ac901211b
  7. Voldman, Joel. "Electrical forces for microscale cell manipulation." Annu. Rev. Biomed. Eng., vol. 8 pp. 425-454, 2006. https://doi.org/10.1146/annurev.bioeng.8.061505.095739
  8. S.W. Lee, Haibo Li, and Rashid Bashir. "Dielectrophoretic tweezers for examining particle-surface interactions within microfluidic devices." Applied physics letters, vol. 90, pp. 223902-223902, 2007 https://doi.org/10.1063/1.2744483
  9. Pethig, R. and G. H. Markx., "Applications of dielectrophoresis in biotechnology." Trends in biotechnology, vol. 15(10), pp. 426-432, 1997. https://doi.org/10.1016/S0167-7799(97)01096-2
  10. Chen, J., M. Abdelgawad, et al. "Electrodeformation for single cell mechanical characterization." JOURNAL OF MICROMECHANICS AND MICROENGINEERING., vol. 21.5, 054012, 2011 https://doi.org/10.1088/0960-1317/21/5/054012
  11. T.W. Lee, K. Nam, et al. "Numerical and Experimental Study on Dielectrophoretic and Electrohydrodynamic Traps using Micro-Particles on an Interdigitated Electrode Array System." International Journal of Nonlinear Sciences and Numerical Simulation, vol. 11(10), pp. 777-784, 2010.
  12. Manaresi, N., A. Romani, et al. "A CMOS chip for individual cell manipulation and detection." Solid-State Circuits, IEEE Journal of vol. 38(12), pp. 2297-2305, 2003 https://doi.org/10.1109/JSSC.2003.819171
  13. T.J. Kwak, J.W. Lee, D.S. Yoon, S.W. Lee. "Investigation of the Binding Force between Protein A and Immunoglobulin G Using Dielectrophoretic(DEP) Tweezers Inside a Microfluidic Chip" Journal of Biomedical Engineering Research, vol. 34, pp. 123-128, 2013 https://doi.org/10.9718/JBER.2013.34.3.123
  14. Markx, G. H., J. Rousselet, et al. "DEP-FFF: Field-flow fractionation using non-uniform electric fields." Journal of liquid chromatography & related technologies, vol. 20(16-17), pp. 2857-2872, 1997 https://doi.org/10.1080/10826079708005597
  15. Huang, Y., X. B. Wang, et al. "Introducing dielectrophoresis as a new force field for field-flow fractionation." Biophysical journal, vol. 73(2), pp. 1118-1129, 1997. https://doi.org/10.1016/S0006-3495(97)78144-X