Journal of Ginseng Research

  • 제37권1호
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  • Pages.80-86
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  • 2013
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  • 1226-8453(pISSN)
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  • 2093-4947(eISSN)
고려인삼학회 (The Korean Society of Ginseng)
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Protective effect of ginsenoside-Rb2 from Korean red ginseng on the lethal infection of haemagglutinating virus of Japan in mice

  • Yoo, Yung Choon (Department of Microbiology, College of Medicine, Konyang University) ;
  • Lee, Junglim (Department of Microbiology, College of Medicine, Konyang University) ;
  • Park, Seok Rae (Department of Microbiology, College of Medicine, Konyang University) ;
  • Nam, Ki Yeul (College of Agriculture and Life Science, Chungnam National University) ;
  • Cho, Young Ho (Department of Pharmaceutical Engineering, Konyang University) ;
  • Choi, Jae Eul (College of Agriculture and Life Science, Chungnam National University)
  • 투고 : 2012.08.27
  • 심사 : 2012.09.18
  • 발행 : 2013.01.15
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초록

Korean red ginseng has been shown to possess a variety of biological activities. However, little is known about antiviral activity of ginsenosides of Korean red ginseng. Here, we investigated the protective effect by oral administration of various ginsenosides on the lethal infection of haemagglutinating virus of Japan (HVJ) in mice. In a lethal infection model in which almost all mice infected with HVJ died within 15 days, the mice were administered orally (per os) with 1 mg/mouse of dammarane-type (ginsenoside-Rb1, -Rb2, -Rd, -Re, and -Rg2) or oleanolic acid-type (ginsenoside-Ro) ginsenosides 3, 2, and 1 d before virus infection. Ginsenoside-Rb2 showed the highest protective activity, although other dammarane-type and oleanolic acid-type ginsenosides also induced a significant protection against HVJ. However, neither the consecutive administration with a lower dosage (300 ${\mu}g$/mouse) nor the single administration of ginsenoside-Rb2 (1 mg/mouse) was active. In comparison of the protective activity between ginsenoside-Rb2 and its two hydrolytic products [20(S)- and 20(R)-ginsenoside-Rg3], 20(S)-ginsenoside-Rg3, but not 20(R)-ginsenoside-Rg3, elicited a partial protection against HVJ. The protective effect of ginsenoside-Rb2 and 20(S)-ginsenoside-Rg3 on HVJ infection was confirmed by the reduction of virus titers in the lungs of HVJ-infected mice. These results suggest that ginsenoside-Rb2 is the most effective among ginsenosides from red ginseng to prevent the lethal infection of HVJ, so that this ginsenoside is a promising candidate as a mucosal immunoadjuvant to enhance antiviral activity.

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참고문헌

  1. Song X, Hu S. Adjuvant activities of saponins from traditional Chinese medicinal herbs. Vaccine 2009;27:4883-4890. https://doi.org/10.1016/j.vaccine.2009.06.033
  2. Sun HX, Xie Y, Ye YP. Advances in saponin-based adjuvants. Vaccine 2009;27:1787-1796. https://doi.org/10.1016/j.vaccine.2009.01.091
  3. Kirk DD, Rempel R, Pinkhasov J, Walmsley AM. Application of Quillaja saponaria extracts as oral adjuvants for plant-made vaccines. Expert Opin Biol Ther 2004;4:947-958. https://doi.org/10.1517/14712598.4.6.947
  4. Pickering RJ, Smith SD, Strugnell RA, Wesselingh SL, Webster DE. Crude saponins improve the immune response to an oral plant-made measles vaccine. Vaccine 2006;24:144-150. https://doi.org/10.1016/j.vaccine.2005.07.097
  5. Park D, Bae DK, Jeon JH, Lee J, Oh N, Yang G, Yang YH, Kim TK, Song J, Lee SH et al. Immunopotentiation and antitumor effects of a ginsenoside Rg3-fortified red ginseng preparation in mice bearing H460 lung cancer cells. Environ Toxicol Pharmacol 2011;31:397-405. https://doi.org/10.1016/j.etap.2011.01.008
  6. Yu JL, Dou DQ, Chen XH, Yang HZ, Guo N, Cheng GF. Protopanaxatriol-type ginsenosides differentially modulate type 1 and type 2 cytokines production from murine splenocytes. Planta Med 2005;71:202-207. https://doi.org/10.1055/s-2005-837817
  7. Dong H, Bai LP, Wong VK, Zhou H, Wang JR, Liu Y, Jiang ZH, Liu L. The in vitro structure-related anti-cancer activity of ginsenosides and their derivatives. Molecules 2011;16:10619-10630. https://doi.org/10.3390/molecules161210619
  8. Mochizuki M, Yoo YC, Matsuzawa K, Sato K, Saiki I, Tono-oka S, Samukawa K, Azuma I. Inhibitory effect of tumor metastasis in mice by saponins, ginsenoside-Rb2, 20(R)- and 20(S)-ginsenoside-Rg3, of red ginseng. Biol Pharm Bull 1995;18:1197-1202. https://doi.org/10.1248/bpb.18.1197
  9. Musende AG, Eberding A, Wood CA, Adomat H, Fazli L, Hurtado-Coll A, Jia W, Bally MB, Tomlinson Guns ES. A novel oral dosage formulation of the ginsenoside aglycone protopanaxadiol exhibits therapeutic activity against a hormone-insensitive model of prostate cancer. Anticancer Drugs 2012;23:543-552. https://doi.org/10.1097/CAD.0b013e32835006f5
  10. Kim JY, Kim HJ, Kim HJ. Effect of oral administration of Korean red ginseng on influenza A (H1N1) virus infection. J Ginseng Res 2011;35:104-110. https://doi.org/10.5142/jgr.2011.35.1.104
  11. Yoo DG, Kim MC, Park MK, Song JM, Quan FS, Park KM, Cho YK, Kang SM. Protective effect of Korean red ginseng extract on the infections by H1N1 and H3N2 influenza viruses in mice. J Med Food 2012;15:855-862. https://doi.org/10.1089/jmf.2012.0017
  12. Lee MH, Lee BH, Jung JY, Cheon DS, Kim KT, Choi CS. Antiviral effect of Korean red ginseng extract and ginsenosides on murine norovirus and feline calicivirus as surrogates for human norovirus. J Ginseng Res 2011;35:429-435. https://doi.org/10.5142/jgr.2011.35.4.429
  13. Cho JY, Kim AR, Yoo ES, Baik KU, Park MH. Ginsenosides from Panax ginseng differentially regulate lymphocyte proliferation. Planta Med 2002;68:497-500. https://doi.org/10.1055/s-2002-32556
  14. Lee KT, Jung TW, Lee HJ, Kim SG, Shin YS, Whang WK. The antidiabetic effect of ginsenoside Rb2 via activation of AMPK. Arch Pharm Res 2011;34:1201-1208. https://doi.org/10.1007/s12272-011-0719-6
  15. Sato K, Mochizuki M, Saiki I, Yoo YC, Samukawa K, Azuma I. Inhibition of tumor angiogenesis and metastasis by a saponin of Panax ginseng, ginsenoside-Rb2. Biol Pharm Bull 1994;17:635-639. https://doi.org/10.1248/bpb.17.635
  16. Fukushima A, Yoo YC, Yoshimatsu K, Matsuzawa K, Tamura M, Tono-oka S, Taniguchi K, Urasawa S, Arikawa J, Azuma I. Effect of MDP-Lys(L18) as a mucosal immunoadjuvant on protection of mucosal infections by Sendai virus and rotavirus. Vaccine 1996;14:485-491. https://doi.org/10.1016/0264-410X(95)00236-T
  17. Faisca P, Desmecht D. Sendai virus, the mouse parainfluenza type 1: a longstanding pathogen that remains up-to-date. Res Vet Sci 2007;82:115-125. https://doi.org/10.1016/j.rvsc.2006.03.009
  18. Bomford R, Stapleton M, Winsor S, Beesley JE, Jessup EA, Price KR, Fenwick GR. Adjuvanticity and ISCOM formation by structurally diverse saponins. Vaccine 1992;10:572-577. https://doi.org/10.1016/0264-410X(92)90435-M
  19. Stewart-Tull DE. The theory and practical application of adjuvants. Chichester: Wiley and Sons, 1994.
  20. Samukawa KI, Yamashita H, Matsuda H, Kubo M. Simultaneous analysis of saponins in ginseng radix by high performance liquid chromatography. Chem Pharm Bull (Tokyo) 1995;43:137-141. https://doi.org/10.1248/cpb.43.137
  21. McGhee JR, Mestecky J, Dertzbaugh MT, Eldridge JH, Hirasawa M, Kiyono H. The mucosal immune system: from fundamental concepts to vaccine development. Vaccine 1992;10:75-88. https://doi.org/10.1016/0264-410X(92)90021-B
  22. Yuki Y, Kiyono H. New generation of mucosal adjuvants for the induction of protective immunity. Rev Med Virol 2003;13:293-310. https://doi.org/10.1002/rmv.398
  23. Du XF, Jiang CZ, Wu CF, Won EK, Choung SY. Synergistic immunostimulating activity of pidotimod and red ginseng acidic polysaccharide against cyclophosphamide-induced immunosuppression. Arch Pharm Res 2008;31:1153-1159. https://doi.org/10.1007/s12272-001-1282-6
  24. Yun YS, Moon HS, Oh YR, Jo SK, Kim YJ, Yun TK. Effect of red ginseng on natural killer cell activity in mice with lung adenoma induced by urethan and benzo(a)pyrene. Cancer Detect Prev Suppl 1987;1:301-309.

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  9. Antiviral Effect of Ginsenoside Rb2 and Rb3 Against Bovine Viral Diarrhea Virus and Classical Swine Fever Virus in vitro vol.8, pp.None, 2013, https://doi.org/10.3389/fvets.2021.764909
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