Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Rapid Genotyping of MSTN Gene Polymorphism Using High-resolution Melting for Association Study in Rabbits

  • Peng, Jin (Institute of Animal Genetics and Breeding, Sichuan Agricultural University) ;
  • Zhang, Gong-Wei (Institute of Animal Genetics and Breeding, Sichuan Agricultural University) ;
  • Zhang, Wen-Xiu (Institute of Animal Genetics and Breeding, Sichuan Agricultural University) ;
  • Liu, Yun-Fu (Institute of Animal Genetics and Breeding, Sichuan Agricultural University) ;
  • Yang, Yu (Institute of Animal Genetics and Breeding, Sichuan Agricultural University) ;
  • Lai, Song-Jia (Institute of Animal Genetics and Breeding, Sichuan Agricultural University)
  • Received : 2012.07.09
  • Accepted : 2012.09.10
  • Published : 2013.01.01
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Abstract

The myostatin (MSTN) gene, as a negative regulator of skeletal muscle growth, has been proposed to be associated with production traits in farm animals. In the present study, a T/C variant at -125 bp (relative to ATG start codon) of 5'regulatory region of rabbit MSTN was identified by direct sequencing. Two hundred and twenty two rabbits, which were randomly sampled from 3 breeds (Ira rabbits, Champagne rabbits and Tianfu black rabbits), were genotyped by high-resolution melting (HRM). Comparing the genotyping results of 47 samples with direct sequencing, the HRM showed high sensitivity (0.96) and high specificity (0.98). In the three rabbit breeds, the allele C was the predominant allele. The polymorphic site showed high heterozygosity (He = 0.48) and high effective number of alleles (Ne = 1.91). The genetic diversity was reasonably informative (0.25

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References

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