한국식품위생안전성학회지 (Journal of Food Hygiene and Safety)

  • 제28권4호
  • /
  • Pages.349-353
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  • 2013
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  • 1229-1153(pISSN)
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  • 2465-9223(eISSN)
한국식품위생안전성학회 (The Korean Society of Food Hygiene and Safety)
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훈증소독제, Fumagari OPP®의 Staphylococcus aureus에 대한 살균효과

Bactericidal Efficacy of Fumagari OPP®, Fumigant Against Staphylococcus aureus

  • 차춘남 (경상대학교 산업시스템공학부 공학연구원) ;
  • 박은기 (고신대학교 의과대학 인문사회의학교실) ;
  • 최현주 (인제대학교 임상병리학과) ;
  • 김용팔 (엘캄코바이오(주)) ;
  • 유창열 (경남도립남해대학 인터넷정보학과) ;
  • 김석 (경상대학교 수의과대학 생명과학연구원) ;
  • 이후장 (경상대학교 수의과대학 생명과학연구원)
  • Cha, Chun-Nam (Engineering Research Institute and Department of Industrial Systems Engineering, Gyeongsang National University) ;
  • Park, Eun-Kee (Department of Medical Humanities and Social Medicine, College of Medicine, Kosin University) ;
  • Choi, Hyunju (Elderly Life Redesign Institute and Department of Biomedical Laboratory Science, Inje University) ;
  • Kim, Yongpal (Elkahmco Bio Co., Ltd.) ;
  • Yoo, Chang-Yeol (Department of Computer Information, Gyeongnam Provincial Namhae College) ;
  • Kim, Suk (Research Institute of Life Sciences, College of Veterinary Medicine, Gyeongsang National University) ;
  • Lee, Hu-Jang (Research Institute of Life Sciences, College of Veterinary Medicine, Gyeongsang National University)
  • 투고 : 2013.09.16
  • 심사 : 2013.11.05
  • 발행 : 2013.12.31
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초록

본 연구는 20% ortho-phenylphenol을 함유한 훈증소독제, Fumagari OPP$^{(R)}$의 Staphylococcus aureus (S. aureus)에 대한 살균효과를 평가하기 위해, French standard NF T 72-281에 따라 수행하였다. 배양 현탁액 중 S. aureus의 균수 (N 값), 훈증소독제에 노출된 각 담체의 균수 (n1, n2, n3), 평판배지법에 의한 시험균주 현탁액 중 균수 (N1), 여과법에 의한 시험균주 현탁액 중 균수 (N2), 그리고 대조담체의 회복 균수의 평균값 (T 값)을 예비실험을 통해 구하였다. 또한, 훈증소독제에 노출된 S. aureus의 감소 균수 (d 값)는, T 값, 회복액 중 균수의 평균값 (n'1) 그리고 배지의 담체에서 증식한 균수의 평균값 (n'2) 등을 이용하여 산출하였다. N 값은 $4.0{\times}10^8$ CFU/mL이었으며, n1, n2, n3은 각각 0.5N1, 0.5N2, 0.5N1 보다 높게 나타났다. 그리고 T값은 $3.4{\times}10^6$ CFU/mL이었다. 훈증소독제의 살균효과에 있어서, d 값은 6.43 logCFU/mL이었다. 훈증소독제에 대한 프랑스 기준에 따르면, 효과적인 살균력을 갖는 훈증소독제의 d 값이 5 logCFU/mL 이상이어야 하는 것으로 규정하고 있다. 본 연구의 결과로부터, Fumagari OPP$^{(R)}$는 S. aureus에 대해 높은 살균효과를 갖는 것으로 나타나, 병원성 세균으로 오염된 식품재료와 주방기기의 소독에 적용할 수 있을 것으로 사료된다.

This study was performed to evaluate the bactericidal efficacy of Fumagari OPP$^{(R)}$, fumigation disinfectant, containing 20% ortho-phenylphenol against Staphylococcus aureus (S. aureus). In this research, efficacy test of fumigant against S. aureus was carried out according to French standard NF T 72-281. S. aureus working culture suspension number (N value), all of the colony numbers on the carriers exposed with the fumigant (n1, n2, and n3), the number of bacterial test suspentions by pour plate method (N1), the number of bacterial test suspentions by filter membrane method (N2) and the mean number of bacteria recovered on the control-carriers (T value) were obtained from the preliminary test. In addition, the reduction number of S. aureus exposed with the fumigant (d value) was calculated using T value, the mean number of bacteria in recovery solution (n'1) and the mean number of bacteria on carriers plated in agar (n'2). N value was $4.0{\times}10^8$ CFU/mL, and n1, n2, and n3 were higher than 0.5N1, 0.5N2 and 0.5N1, respectively. Additionally, T value was $3.4{\times}10^6$ CFU/mL. In the bactericidal effect of the fumigant, the d value was 6.43 logCFU/mL. According to the French standard for the fumigant, the d value for the effective bactericidal fumigant should be over than 5 logCFU/mL. The results indicated that Fumagari OPP$^{(R)}$ had an effective bactericidal activity against S. aureus, then the fumigant can be applied to disinfect food materials and kitchen appliances contaminated with pathogenic bacteria.

키워드

참고문헌

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피인용 문헌

  1. Antibacterial Activity of Aqueous Garlic Extract Against Escherichia coli O157:H7, Salmonella typhimurium and Staphylococcus aureus vol.30, pp.2, 2015, https://doi.org/10.13103/JFHS.2015.30.2.210