Journal of Forest and Environmental Science

Institute of Forest Science, kangwon National University (강원대학교 산림과학연구소)
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Anti-Inflammatory Effects of Xanthoceras sorbifolia Seeds Oil on RAW264.7 Macrophages and TPA-Induced Ear Edema Mice

RAW264.7 대식세포와 급성염증유발 동물모델에서 문관나무 종자유의 염증억제 효과

  • Jeong, Hye Jeong (Agriproduct Processing Experiment Station Gangwon-do Agricultural Research and Experiment Services) ;
  • Lee, Ki Yeon (Agriproduct Processing Experiment Station Gangwon-do Agricultural Research and Experiment Services) ;
  • Hong, Soo Young (Agriproduct Processing Experiment Station Gangwon-do Agricultural Research and Experiment Services) ;
  • Heo, Nam Ki (Agriproduct Processing Experiment Station Gangwon-do Agricultural Research and Experiment Services) ;
  • Kim, Hee Yeon (Agriproduct Processing Experiment Station Gangwon-do Agricultural Research and Experiment Services)
  • Received : 2013.11.19
  • Accepted : 2013.11.22
  • Published : 2013.11.30
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Abstract

This study investigated the anti-inflammatory effects of Xanthoceras sorbifolia of seeds oil on RAW264.7 macrophages and TPA (12-O-tetra decanoylphorbol-acetate)-induced ear edema mice. MTT assay method to measure cytotoxicity was formed in RAW264.7 cell. The anti-inflammatory effect was measured by ability to inhibit production nitric oxide (NO) in RAW264.7 cell. Hexane and eight-percent methanol fractions from Xanthoceras sorbifolia seeds oil did not show cytotoxicity. Hexane and eight-percent methanol fractions were showed significantly inhibitory effect on NO production. TPA-induced acute edema was developed in the mouse ears, and Xanthoceras sorbifolia seeds oil dissolved in acetone was applied to inflamed ears. It was found out that Xanthoceras sorbifolia seeds oil could significantly reduce th ear swelling, compared to the control. Overall results indicate that the Xanthoceras sorbifolia seeds oil has anti-inflammatory activity and could be used as a resource of anti-inflammatory materials.

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References

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