Journal of the Korean Wood Science and Technology

The Korean Society of Wood Science & Technology (한국목재공학회)
권호 표지
DOI QR코드

DOI QR Code

Utilization of Light Microscopy and FFT for MFA Measurement from Unstained Sections of Red Pine (Pinus Densiflora)

  • Kwon, Ohkyung (Nanobioimaging Center, National Instrumentation Center for Environmental Management, Seoul National University) ;
  • Lee, Mi-Rim (Nanobioimaging Center, National Instrumentation Center for Environmental Management, Seoul National University) ;
  • Eom, Chang-Deuk (Dept. of Forest Products, Korea Forest Research Institute)
  • Received : 2013.09.02
  • Accepted : 2013.09.24
  • Published : 2013.09.25
Download PDF
⟨ Previous Next ⟩

Abstract

This study demonstrates the utilization of light microscopy and Fast Fourier Transform-Peak Finding (FPF) method for microfibril angle (MFA) measurement from unstained sections of red pine (Pinus densiflora). To obtain an image with optimal contrast and resolution for MFA measurement, effects of numerical aperture (NA) of condenser lens and color filters were investigated. About 60% of NA of the maximum condenser NA produced an image with optimal contrast, but a color filter with short wavelength range (DAPI) created images with improved resolution. Manual angle measurement and the FPF method were applied to the image with optimal contrast for MFA measurement. The experimental results from the FPF method were considered to be more repeatable and less subjective than those from the manual angle measurement.

Keywords

References

  1. Andersson, S., R. Serimaa, M. Torkkeli, T. Paakkari, P. Saranpaa, and E. Pesonen. 2000. Journal of Wood Science 46(5); 343-349. https://doi.org/10.1007/BF00776394
  2. Barnett, J. R. and V. A. Bonham. 2004. Biological Reviews 79(2); 461-472.
  3. Abe, H. and R. Funada. 2005. IAWA Journal 26(2); 161-174. https://doi.org/10.1163/22941932-90000108
  4. Donaldson, L. 2008. IAWA Journal 29(4); 345-386. https://doi.org/10.1163/22941932-90000192
  5. Leney, L. 1981. Wood and Fiber 13(1); 13-16.
  6. Oldenbourg, R. 1999. Methods in Cell Biology 61(61); 175-208.
  7. Abraham, Y. and R. Elbaum. 2013. New Phytologist 197(3); 1012-1019. https://doi.org/10.1111/nph.12070
  8. Peter, G. F., D. M. Benton, and K. Bennett. 2003. Journal of Pulp and Paper Science 29(8); 274-280.
  9. Bergander, A., J. Brandstrom, G. Daniel, and L. Salmen. 2002. Journal of Wood Science 48(4); 255-263. https://doi.org/10.1007/BF00831344
  10. Donaldson, L. and A. Frankland. 2004. Holzforschung 58(3); 219-225.
  11. Sedighi-Gilani, M., H. Sunderland, and P. Navi. 2005. Wood Science and Technology 39(6); 419-430. https://doi.org/10.1007/s00226-005-0022-0
  12. Anagnost, S. E., R. E. Mark, and R. B. Hanna. 2002. Wood and Fiber Science 34(2); 337-349.
  13. Anagnost, S. E., R. E. Mark, and R. B. Hanna. 2000. Wood and Fiber Science 32(1); 81-87.

Cited by

  1. Investigation of Layered Structure of Fiber Cell Wall in Korean Red Pine by Confocal Reflection Microscopy vol.44, pp.2, 2014, https://doi.org/10.9729/AM.2014.44.2.61
  2. Study on the Variations of Inter-annual Tracheid Length for Korean Red Pine from Sokwang-ri in Uljin vol.42, pp.6, 2014, https://doi.org/10.5658/WOOD.2014.42.6.646