Reproductive and Developmental Biology

The Korean Society of Animal Reproduction (한국동물번식학회)
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Transcriptional Profiling of Differentially Expressed Genes in Porcine Satellite Cell

  • Jeong, Jin Young (Division of Animal Genomics and Bioinformatics, National Institute of Animal Science, Rural Development Administration) ;
  • Kim, Jang Mi (Division of Animal Genomics and Bioinformatics, National Institute of Animal Science, Rural Development Administration) ;
  • Rajesh, Ramanna Valmiki (Division of Animal Genomics and Bioinformatics, National Institute of Animal Science, Rural Development Administration) ;
  • Suresh, Sekar (Division of Animal Genomics and Bioinformatics, National Institute of Animal Science, Rural Development Administration) ;
  • Jang, Gul Won (Division of Planning and Coordination, National Institute of Animal Science, Rural Development Administration) ;
  • Lee, Kyung-Tai (Division of Animal Genomics and Bioinformatics, National Institute of Animal Science, Rural Development Administration) ;
  • Kim, Tae Hun (Division of Animal Genomics and Bioinformatics, National Institute of Animal Science, Rural Development Administration) ;
  • Park, Mina (Division of Animal Genomics and Bioinformatics, National Institute of Animal Science, Rural Development Administration) ;
  • Jeong, Hak Jae (Division of Animal Biotechnology, National Institute of Animal Science, Rural Development Administration) ;
  • Kim, Kyung Woon (Division of Animal Biotechnology, National Institute of Animal Science, Rural Development Administration) ;
  • Cho, Yong Min (Division of Animal Genomics and Bioinformatics, National Institute of Animal Science, Rural Development Administration) ;
  • Lee, Hyun-Jeong (Division of Animal Genomics and Bioinformatics, National Institute of Animal Science, Rural Development Administration)
  • Received : 2013.12.03
  • Accepted : 2013.12.17
  • Published : 2013.12.31
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Abstract

Muscle satellite cell (SC) is responsible for postnatal muscle growth, repair, and regeneration. Satellite cell is an important source of multi-potent stem cell process and differentiation into adipogenic, myogenic, and osteoblastogenic. The objective of this study was to identify alter of transcriptome during differentiation in porcine satellite cell and to elevated transcriptome at different stages of postnatal development to gain insight into the differences in differentiated PSC. We used RNA-seq technique to investigate the transcriptomes during differentiation in pig muscle. Sequence reads were obtained from Illumina HiSeq2000. Differentially expressed genes (DEG) were detected by EdgeR. Gene ontology (GO) terms are powerful tool for unification among representation genes or products. In study of GO biological terms, functional annotation clustering involved in cell cycle, apoptosis, extracellular matrix, phosphorylation, proteolysis, and cell signaling in differences stage. Taken together, these results would be contributed to a better understanding of muscle biology and processes underlying differentiation. Our results suggest that the source of DEGs could be better understanding of the mechanism of muscle differentiation and transdifferentiation.

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References

  1. Asakura A, Komaki M, Rudnicki M (2001): Muscle satellite cells are multipotential stem cells that exhibit myogenic, osteogenic, and adipogenic differentiation. Differentiation 68:245-253. https://doi.org/10.1046/j.1432-0436.2001.680412.x
  2. Belu M, Mizutani CM (2011): Variation in mesoderm specification across Drosophilids is compensated by different rates of myoblast fusion during body wall musculature development. LoS One 6:e28970. https://doi.org/10.1371/journal.pone.0028970
  3. Chen TL, Shen WJ, Qiu XW, Li T, Hoffman AR, Kraemer FB (2007): Generation of novel adipocyte monolayer cultures from embryonic stem cells. Stem Cells Dev 16:371-380. https://doi.org/10.1089/scd.2006.0037
  4. Davis TA, Nguyen HV, Suryawan A, Bush JA, Jefferson LS, Kimball SR (2000): Developmental changes in the feeding-induced stimulation of translation initiation in muscle of neonatal pigs. Am J Physiol Endocrinol Metab 279:E1226-34. https://doi.org/10.1152/ajpendo.2000.279.6.E1226
  5. Ding K, Yang Z, Zhang YL, Xu JZ (2013): Injectable thermosensitive chitosan/$\beta$-glycerophosphate/collagen hydrogel maintains the plasticity of skeletal muscle satellite cells and supports their in vivo viability. Cell Biol Int 37:977-987. https://doi.org/10.1002/cbin.10123
  6. Gregoire FM, Smas CM, Sul HS (1998): Understanding adipocyte differentiation. Physiol Rev 78:783-809. https://doi.org/10.1152/physrev.1998.78.3.783
  7. Lee HJ, Jang M, Kim H, Kwak W, Park W, Hwang JY, Lee CK, Jang GW, Park MN, Kim HC, Jeong JY, Seo KS, Kim H, Cho S, Lee BY (2013): Comparative transcriptome analysis of adipose tissues reveals that ECM-receptor interaction is involved in the Depotspecific adipogenesis in cattle. PLoS One 8(6):e662-67.
  8. Phillips BW, Vernochet C, Dani C (2003): Differentiation of embryonic stem cells for pharmacological studies on adipose cells. Pharmacol Res 47:263-268. https://doi.org/10.1016/S1043-6618(03)00035-5
  9. Samulin J, Lien S, Grindflek E, Berget I, Ruyter B (2008): Depot specific differences during adipogenesis of porcine stromal-vascular cells. Cell Biol Int 32:525-531. https://doi.org/10.1016/j.cellbi.2008.01.001
  10. Shi F, Miao J, Zhang L, Tao H, Lü J, Ruan Z, Zong H (2010): Detection of bovine, goat, pig and chicken derived ingredients in animal products with universal PCR-microarray method. Sheng Wu Gong Cheng Xue Bao 26:823-829.
  11. Suryawan A, Nguyen HV, Bush JA, Davis TA (2001): Developmental changes in the feeding-induced activation of the insulin-signaling pathway in neonatal pigs. Am J Physiol Endocrinol Metab 281(5):E908-915. https://doi.org/10.1152/ajpendo.2001.281.5.E908
  12. Tchkonia T, Lenburg M, Thomou T, Giorgadze N, Frampton G, Pirtskhalava T, Cartwright A, Cartwri ght M, Flanagan J, Karagiannides I, Gerry N, Forse RA, Tchoukalova Y, Jensen MD, Pothoulakis C, Kirkland JL (2007): Identification of depot-specific human fat cell progenitors through distinct expression profiles and developmental gene patterns. Am J Physiol Endocrinol Metab 292:E298-307. https://doi.org/10.1152/ajpendo.00202.2006
  13. Zhou G, Wang S, Wang Z, Zhu X, Shu G, Liao W, Yu K, Gao P, Xi Q, Wang X, Zhang Y, Yuan L, Jiang Q (2010): Global comparison of gene expression profiles between intramuscular and subcutaneous adipocytes of neonatal landrace pig using microarray. Meat Sci 86(2):440-450. https://doi.org/10.1016/j.meatsci.2010.05.031