DOI QR코드

DOI QR Code

Antioxidative Activities of Aronia melanocarpa Fruit and Leaf Extracts

아로니아 베리 열매 및 잎 추출물의 항산화 활성

  • Lee, Hye Mi (Department of Fine Chemistry, Cosmetic R&D Center, Nanobiocosmetic Lab., Seoul National University of Science and Technology) ;
  • Kong, Bong Ju (Department of Fine Chemistry, Cosmetic R&D Center, Nanobiocosmetic Lab., Seoul National University of Science and Technology) ;
  • Kwon, Soon Sik (Department of Fine Chemistry, Cosmetic R&D Center, Nanobiocosmetic Lab., Seoul National University of Science and Technology) ;
  • Kim, Kyeong Jin (Department of Fine Chemistry, Cosmetic R&D Center, Nanobiocosmetic Lab., Seoul National University of Science and Technology) ;
  • Kim, Hae Soo (Department of Fine Chemistry, Cosmetic R&D Center, Nanobiocosmetic Lab., Seoul National University of Science and Technology) ;
  • Jeon, So Ha (Department of Fine Chemistry, Cosmetic R&D Center, Nanobiocosmetic Lab., Seoul National University of Science and Technology) ;
  • Ha, Ji Hoon (Department of Fine Chemistry, Cosmetic R&D Center, Nanobiocosmetic Lab., Seoul National University of Science and Technology) ;
  • Kim, Jin-Sook (KNK Co., Ltd.) ;
  • Park, Soo Nam (Department of Fine Chemistry, Cosmetic R&D Center, Nanobiocosmetic Lab., Seoul National University of Science and Technology)
  • 이혜미 (서울과학기술대학교 정밀화학과, 화장품종합기술연구소, 나노바이오화장품연구실) ;
  • 공봉주 (서울과학기술대학교 정밀화학과, 화장품종합기술연구소, 나노바이오화장품연구실) ;
  • 권순식 (서울과학기술대학교 정밀화학과, 화장품종합기술연구소, 나노바이오화장품연구실) ;
  • 김경진 (서울과학기술대학교 정밀화학과, 화장품종합기술연구소, 나노바이오화장품연구실) ;
  • 김해수 (서울과학기술대학교 정밀화학과, 화장품종합기술연구소, 나노바이오화장품연구실) ;
  • 전소하 (서울과학기술대학교 정밀화학과, 화장품종합기술연구소, 나노바이오화장품연구실) ;
  • 하지훈 (서울과학기술대학교 정밀화학과, 화장품종합기술연구소, 나노바이오화장품연구실) ;
  • 김진숙 ((주)케이엔케이) ;
  • 박수남 (서울과학기술대학교 정밀화학과, 화장품종합기술연구소, 나노바이오화장품연구실)
  • Received : 2013.07.25
  • Accepted : 2013.08.14
  • Published : 2013.12.31

Abstract

In this study, the antioxidative effects of Aronia melanocarpa fruit and leaf extracts were investigated. The free radical (1,1-diphenyl-2-picrylhydrazyl, DPPH) scavenging activities ($FSC_{50}$) of the ethylacetate and aglycone fractions of fruit extracts were 16.29 ${\mu}g/mL$, and 12.29 ${\mu}g/mL$, respectively. The free radical scavenging activity of fruit extract was higher than that of leaf extracts. Reactive oxygen species (ROS) scavenging activities ($OSC_{50}$) of the ethylacetate and aglycone fractions of fruit extracts on ROS generated in $Fe^{3+}-EDTA/H_2O_2$ system using the luminol-dependent chemiluminescence assay showed 2.86 ${\mu}g/mL$, and 1.80 ${\mu}g/mL$, respectively. ROS scavenging activity of the aglycone fraction of fruit extracts was similar to that of L-ascorbic acid (1.50 ${\mu}g/mL$). The ROS scavenging activity of fruit extracts was higher than that of leaf extracts. The cellular protective effects of aglycone fraction of fruit extracts (${\tau}_{50}$ = 72.3 min) on the $^1O_2$-induced cellular damage of human erythrocytes especially were increased in a concentration dependent manner (5 ~ 50 ${\mu}g/mL$). ${\tau}_{50}$ (72.3 min) of the aglycone fraction showed 1.9 times higher than (+)-${\alpha}$-tocopherol (38 min), known as lipophilic antioxidant at 10 ${\mu}g/mL$. These results incidicate that A. melanocarpa fruit extracts have higher antioxidant effects than leaf extracts and could be applicable to functional cosmetics materials for antioxidants by protecting skin exposed to solar UV radiation against ROS including $^1O_2$.

본 연구에서는 아로니아 베리 열매 및 잎 추출물의 항산화 활성을 비교 연구하였다. 아로니아 베리 열매의 에틸아세테이트 분획 및 아글리콘 분획의 free radical 소거 활성($FSC_{50}$)은 각각 16.29 ${\mu}g/mL$ 및 12.29 ${\mu}g/mL$이었으며, 열매추출물의 free radical 소거활성은 잎 추출물 경우보다 높게 나타났다. Luminol-의존성 화학발광법을 이용한 $Fe^{3+}-EDTA/H_2O_2$계에서 생성된 활성산소종(reactive oxygen species, ROS)에 대한 아로니아 베리 열매 추출물의 총 항산화능($OSC_{50}$)은 에틸아세테이트 분획의 경우 2.86 ${\mu}g/mL$, 아글리콘 분획은 1.80 ${\mu}g/mL$로, 아글리콘 분획의 총항산화능은 ascorbic acid (1.50 ${\mu}g/mL$)와 비슷한 활성을 나타내었다. 총항산화능에서도 열매 추출물이 잎 추출물 보다 활성이 높은 것으로 나타났다. 사람 적혈구의 $^1O_2$로 유도된 세포손상에 대한 보호 효과 실험에서 열매의 아글리콘 분획은 농도의존적(5 ~ 50 ${\mu}g/mL$)으로 세포보호 효과를 나타내었다. 열매 아글리콘분획의 ${\tau}_{50}$은 10 ${\mu}g/mL$에서 72.3 min으로 지용성 항산화제로 알려진 (+)-${\alpha}$-tocopherol (38.0 min)보다 1.9 배 더 큰 세포보호 효과를 나타내었다. 이상의 결과들은 아로니아 베리의 열매 추출물이 잎 추출물보다 높은 항산화능을 나타내며, 태양 자외선에 노출된 피부에서 발생되는 $^1O_2$를 포함하는 ROS에 대항하여 세포를 보호함으로써 기능성 항산화 화장품 소재로서의 응용 가능성이 있음을 시사한다.

Keywords

References

  1. S. N. Park, Skin aging and antioxidant, J. Soc. Cosmet. Scientists Korea, 23(3), 75 (1997).
  2. S. N. Park, Antioxidative properties of baicalein, component from Scutellaria baicalensis Georgi and its application to cosmetics (I), J. Korean Ind. Eng. Chem., 14(5), 657 (2003).
  3. H. Masaki, Role of antioxidants in the skin : Antiaging effects, J. Dermatol. Sci., 58, 85 (2010). https://doi.org/10.1016/j.jdermsci.2010.03.003
  4. L. Packer, Ultraviolet radiation (UVA, UVB) and skin antioxidants, In Free Radical Damage and Its Control, eds. C. A. Rice-Evans and R. H. Burdon, 239 Elsevier Science B. (1994).
  5. K. J. A. Davies, Protein damage and degradation by oxygen radical, J. Biol. Chem., 262, 9895 (1987).
  6. O. ten Berge, S. G. A. van Veisen, B. Glovannone, C. A.F.M. Bruljnzeel-Koomen, E. F. Knol, K. Guikers, and H. van Weelden, Assessment of cyclobutane pyrimidine dimers by digital photography in human skin, J. Immun. Methods, 373(1-2), 240 (2011). https://doi.org/10.1016/j.jim.2011.07.014
  7. H. M. Chiang, H. C. Chen, H. H. Chiu, C. W. Chen, S. M. Wang, and K. C. Wen, Neonauclea reticulata (Havil.) Merr stimulates skin regeneration after UVB exposure via ROS scavenging and modulation of the MAPK/MMPs/collagen pathway, Evid. Based Complement. Alternat. Med., 2013, 9 (2013).
  8. K. Scharffetter-Kochanek, M. Wlaschek, K. Briviba, and H. Sies, Singlet oxygen induces collagenase expression in human skin fibroblasts, FEBS Lett., 331, 304 (1993). https://doi.org/10.1016/0014-5793(93)80357-Z
  9. M. Wlaschek, K. Briviba, G. P. Stricklin, H. Sies, and K. Scharffetter-Kochanek, Singlet oxygen may mediate the ultraviolet A induced synthesis of intestitial collagenase, J. Invest. Dermatol., 104, 194 (1995). https://doi.org/10.1111/1523-1747.ep12612751
  10. J. E. Kim, H. J. Lee, M. S. Lim, M. A. Park, and S. N. Park, Cellular protective effect and liposome formulation for enhanced transdermal delivery of Persicaria hydropiper L. extract, J. Soc. Cosmet. Scientists Korea, 38(1), 15 (2012). https://doi.org/10.15230/SCSK.2012.38.1.015
  11. S. N. Park, H. J. Lee, H. S. Kim, M. A. Park, and H. A. Gu, Enhanced transdermal deposition and characterization of quercetin-loaded ethosomes, Korean J. Chem. Eng., 30(3), 688 (2013). https://doi.org/10.1007/s11814-012-0171-4
  12. M. H. Lee, S. J. Kim, and S. N. Park, Development of porous cellulose-hydrogel system for enhanced transdermal delivery of quercetin and rutin, Polymer (Korea), 37(3), 347 (2013). https://doi.org/10.7317/pk.2013.37.3.347
  13. S. N. Park, M. H. Lee, S. J. Kim, and E. R. Yu, Preparation of quercetin and rutin-loaded ceramide liposomes and drug-releasing effect in liposomein- hydrogel complex system, Biochem. Biophys. Res. Commun., 435, 361 (2013). https://doi.org/10.1016/j.bbrc.2013.04.093
  14. T. Tanaka and A. Tanaka, Chemical components and characteristics of black chokeberry, J. Jpn. Soc. Food Sci. Technol., 48, 606 (2001). https://doi.org/10.3136/nskkk.48.606
  15. J. Hudec, D. Bakosy, D. Mravec, L. Kobida, M. Burdovaa, I. Turianica, and J. Hlusyek, Content of phenolic compounds and free polyamines in black chokeberry (Aronia melanocarpa) after application of polyamine biosynthesis regulators, J. Agric. Food Chem., 54, 3625 (2006). https://doi.org/10.1021/jf060299q
  16. L. Sueiro, G. G. Yousef, D. Seigler, E. G. DE Mejia, M. H. Grace, and M. A. Lila, Chemopreventive potential of flavonoid extracts from plantation-bred and wild Aronia melanocarpa (black chokeberry) fruits, J. Food Sci., 71(8), C480 (2006). https://doi.org/10.1111/j.1750-3841.2006.00152.x
  17. A. Kokotkiewicz, Z. Jaremicz, and M. Luczkiewicz, Aronia plants: A review of traditional use, biological activities, and perspectives for modern medicine, J. Med. Food, 13(2), 255 (2010). https://doi.org/10.1089/jmf.2009.0062
  18. J. Niedworok, B Jankowska, E. Kowalczyk, K. Charyk, and Z. Kubat, Antiulcer activity of anthocyanin from Aronia melanocarpa Elliot, Herba Polonica, 43, 222 (1997).
  19. K. Ohgami, I. Ilieva, K. Shiratori, Y. Koyoma, X. Jin, K. Yoshida, S. Kase, N. Kitaichi, Y. Suzuki, T. Tanaka, and S. Ohno, Anti-inflammatory effect of aronia extract on rat endotoxin-induced uveitis, Invest. Ophthalmol. Vis. Sci., 46, 275 (2005). https://doi.org/10.1167/iovs.04-0715

Cited by

  1. Quality Characteristics and Antioxidant Activities of Yanggaeng with Aronia Leaf Powder vol.33, pp.6, 2017, https://doi.org/10.9724/kfcs.2017.33.6.654
  2. Antioxidant Activities of Ipomoea batatas L. Lam. (Purple Sweet Potato) Extracts Cultured in Korea vol.40, pp.4, 2014, https://doi.org/10.15230/SCSK.2014.40.4.423
  3. Effects of Different Ripening Stage and Varieties on Quality Characteristics and Antioxidant Activity of Aronia (Aronia Melaocarpa) vol.22, pp.4, 2018, https://doi.org/10.13050/foodengprog.2018.22.4.374