Korean Journal of Fisheries and Aquatic Sciences (한국수산과학회지)

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Availability of the lacZ gene as a Reporter Gene for Production of Transgenic Artemia franciscana

형질전환 아르테미아(Artemia franciscana) 생산을 위한 리포터 유전자로서 lacZ 유전자의 유용성 검토

  • Jung, Hyo Sun (Risk Assessment Center of Marine Living Modified Organisms, Pukyong National University) ;
  • Kim, Dong Soo (Department of Marine Bio-Materials & Aquaculture, Pukyong National University)
  • 정효선 (부경대학교 해양용 LMO 위해성 평가 센터) ;
  • 김동수 (부경대학교 해양바이오신소재학과)
  • Received : 2013.05.08
  • Accepted : 2013.09.25
  • Published : 2013.12.31
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Abstract

We examined the availability of the lacZ gene (${\beta}$-galactosidase gene) as a reporter of foreign gene transfer in the cysts of Artemia franciscana (A. franciscana) to conduct a risk assessment of living genetically modified organisms (LMOs) in the marine ecosystem. The LacZ gene was transferred to decapsulated cysts by particle bombardment, and its insertion and expression were assessed by means of polymerase chain reaction (PCR) and X-gal staining. X-gal staining indicated lacZ expression in all A. franciscana examined (including the control group), which exhibited not only negative but also positive PCR amplification. Endogenous ${\beta}$-galactosidase is highly active in the whole body of A. franciscana during all stages of the life cycle. Thus, the lacZ gene is unsuitable as a reporter for foreign gene transfer in A. franciscana cysts, because it is difficult to discriminate between exogenous and endogenous ${\beta}$-galactosidase activity.

Keywords

References

  1. Adam A, Bartfai R, Lele Z, Krone PH and Orban L. 2000. Heat-inducible expression of a reporter gene detected by transient assay in zebrafish. Exp Cell Res 256, 282-290. https://doi.org/10.1006/excr.2000.4805
  2. Arenal A, Pimentel R, Garcia C, Pimentel E and Alestrom P. 2004. The SV40 T antigen nuclear localization sequence enhances nuclear import of vector DNA in embryos of a crustacean (Litopenaeus schmitti). Gene 337, 71-77. http:// dx.doi.org/10.1016/j.gene.2004.04.007.
  3. Clegg JS and Trotman CAN. 2002. Physiological and biochemi­cal aspects of Artemia ecology. In: ARTEMIA: Basic and applied biology of aquatic organisms, Abatzopoulos TJ, Beardmore JA, Clegg JS and Sorgeloos P, eds. Kluwer Aca­demic Publishers, USA, 129-170.
  4. Dube A, Gupta R and Singh N. 2009. Reporter genes facili­tating discovery of drugs targeting protozoan parasites. Trends Parasitol 25, 432-439. http://dx.doi.org/10.1016/j. pt.2009.06.006.
  5. Gendreau S, Lardans V, Cadoret JP and Mialhe E. 1995. Tran­sient expression of a luciferase reporter gene after ballistic introduction into Artemia franciscana (Crustacea) embryos. Aquaculture 133, 199-205. https://doi.org/10.1016/0044-8486(94)00369-Y
  6. Hostetler HA, Peck SL and Muir WM. 2003. High efficiency production of germ-line transgenic Japanese medaka (Ory­zias latipes) by electroporation with direct current-shifted radio frequency pulses. Transgenic Res 12, 413-424. http:// dx.doi.org/10.1023/A:1024248300592.
  7. Jiang T, Xing B and Rao J. 2008. Recent developments of biological reporter technology for detecting gene expres­sion. Biotechnol Genet Eng Rev 25, 41-75. http://dx.doi. org/10.5661/bger-25-41.
  8. Jurado E, Camacho F, Luzon G and Vicaria JM. 2004. Kinetic models of activity for $\beta$-galactosidases: influence of pH, ionic concentration and temperature. Enzyme Microbial Technol 34, 33-40. https://doi.org/10.1016/j.enzmictec.2003.07.004
  9. Kopp HG, Hooper AT, Shmelkov SV and Rafii S. 2007. $\beta$-Ga­lactosidase staining on bone marrow. The osteoclast pitfall. Histol Histopathol 22, 971-976.
  10. Kim DH, Jeong CH, Nam YK, Min KS and Kim DS. 1996. Com­parative study on endogeneous activities of $\beta$-galactosidase­like enzyme in several finfishes and shellfishes. J Aquacult 9, 445-452.
  11. Kim DS and Nam TK. 1994. Transfer of foreign gene into mud louch, Misgurnus mizolepis. I. Availablility of the LacZ as a reporter gene for production transgenic mud loachJ Aqua-cult 7, 41-54.
  12. Lopez-Galindo C, Garrido MC, Casanueva JF and Nebot E. 2010. Degradation models and ecotoxicity in marine waters of two antifouling compounds:Sodium hypochlorite and an alkylamine surfactant. Sci Total Environ 408, 1779-1785. http://dx.doi.org/10.1016/j.scitotenv.2010.01.029.
  13. Moretti A, Pedini Fernandez-Criado M, Cittolin G and Guidastri R. 1999. Manual on hatchery production of seabass and gil­thead seabream. FAO, Rome, Italy, 194.
  14. Nam YK, CH Jeong and DS Kim. 1998. A simple clearing method for improves visualization of LacZ expression in fish larvae. Transgenic Res 7, 397-399. https://doi.org/10.1023/A:1008804619393
  15. Nambu Z, Tanaka S and Nambu F. 2004. Influence of photo-period and temperature on reproductive mode in the brine shrimp, Artemia franciscana. J Exp Zool 301, 542-546. http://dx.doi.org/10.1002/jez.a.80.
  16. Sanford JC, Smith FD and Russell JA. 1993. Optimizing the bi­olistic process for different biological applications. Methods Enzymol 217, 483-509. https://doi.org/10.1016/0076-6879(93)17086-K
  17. Shimada A, Komatsu K, Nakashima K, Poschl E and Nifuji A. 2012. Improved methods for detection of $\beta$-galactosidase (lacZ) activity in hard tissue. Histochem Cell Biol 137, 841­-847. http://dx.doi.org/10.1007/s00418-012-0936-1.
  18. Sin FYT. 1997. Transgenic fish. Rev Fish Biol Fisher 7, 417-­441. https://doi.org/10.1023/A:1018452214763
  19. Sorgeloos P, Etienne B, Einstein L, Marite BM and Guido P. 1977. Decapsulation of Artemia cysts: A simple technique for the improvement of the use of brine shrimp in aquacul­ture. Aquaculture 12, 311-315. https://doi.org/10.1016/0044-8486(77)90209-5
  20. Van Stappen G. 1996. Introduction, biology and ecology of Ar­temia. In: Manual on the production and use of live food for aquaculture. Lavens P and Sorgeloos P, eds. Food and Ag­riculture Organization of the United Nations, Rome, Italy, 79-106.
  21. Verri T, Ingrosso L, Chiloiro R, Danieli A, Zonno V, Alifano P, Romano N, Scapigliati G, Vilella S and Storelli C. 2003. As­sessment of DNA vaccine potential for gilthead sea bream (Sparus aurata) by intramuscular injection of a reporter gene. Fish Shellfish Immunol 15, 283-295. https://doi.org/10.1016/S1050-4648(02)00166-3
  22. Ueno K, Hamaguchi S, Ozato K, Kang J and Inoue K. 1994. Foreign gene transfer into nigorobuna (Carassius auratus grandoculis). Mol Mar Biol Biotechnol 3, 235-342.
  23. Weiss DJ, Liggitt D and Clark JG. 1999. Histochemical dis­crimination of endogenous mammalian beta-galactosidase activity from that resulting from lac-Z gene expression. His­tochem J 31, 231-236. https://doi.org/10.1023/A:1003642025421
  24. Zheng FR, Sun XQ, Liu HZ and Zhang JX. 2006. Study on the distribution and expression of a DNA vaccine against lymphocystis disease virus in Japanese flounder (Paralich­thys olivaceus). Aquaculture 261, 1128-1134. http://dx.doi. org/10.1016/j.aquaculture.2006.05.020.