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Effects of Krill Euphausia superba Fluoride Extract on Toxicity and Oxidative Stress in Liver cell

크릴(Euphausia superba) 불소 추출물의 간세포 독성 및 산화적 스트레스에 미치는 영향

  • Kim, Jeong Gyun (Department of Food Science & Technology/Institute of Marine Industry, Gyeongsang National University) ;
  • Yoon, Ho Dong (Southeast Sea Fisheries Research Institute, National Fisheries Research and Development Institute) ;
  • Park, Sihyang (Sun marine Biotechnology Co.) ;
  • Kim, Poong Ho (Southeast Sea Fisheries Research Institute, National Fisheries Research and Development Institute) ;
  • Mok, Jong Soo (Southeast Sea Fisheries Research Institute, National Fisheries Research and Development Institute) ;
  • Hong, Yumi (Sun marine Biotechnology Co.)
  • 김정균 (경상대학교 해양과학대학 해양식품공학과) ;
  • 윤호동 (국립수산과학원 남동해수산연구소) ;
  • 박시향 (선마린바이오테크) ;
  • 김풍호 (국립수산과학원 남동해수산연구소) ;
  • 목종수 (국립수산과학원 남동해수산연구소) ;
  • 홍유미 (선마린바이오테크)
  • Received : 2013.09.09
  • Accepted : 2013.11.20
  • Published : 2013.12.31

Abstract

In this study, we investigated about cell toxicity and oxidative stress of HepG2 cell by treatment of sodium fluoride (NaF) and fluoride extracts from krill Euphausia superba meat, shell, whole body and krill meal. The cell toxicity showed significant at 300 and $500{\mu}g/mL$ NaF treatment group. But krill (Euphausia superba) fluoride extract (KFE) treatment in all groups were not toxic. The superoxide radical production increased significantly in NaF treated group, but there was no significant change in KFE treated group. The superoxide dismutase activity was a significant increase 21.5% at $100{\mu}g/mL$ and 24.7% at $300{\mu}g/mL$ treatment group of fluoride extracts from krill meat, and 8.7% at $300{\mu}g/mL$ in krill meals, compared to the control group. However, hydroxy radical flux and catalase and glutathione peroxidase activity of fluoride extracts from krill meat did not change. As a result, for a short period of time, NaF treatment in HepG2 cells affect the cell toxicity and oxidative stress, but in the case of KFE, these were not recognized. Thus, depending on the type of food ingested with fluoride, cell toxicity and oxidative stress was found to be different.

Keywords

References

  1. Tou JC, Jaczynski and Chen YC. 2007. Krill for Human consumption: Nutritional value and petential health benefits. Nutrion Reviews 65, 63-77. http://dx.doi. org/10.1111/j.1753-4887.2007.tb00283.x.
  2. Park HJ, Ham KS, Kim DM and Kim KH. 1988. Decrease of fluoride content of Antarctic krill. Korean J Food Sci Tech­nol 20, 19-22.
  3. Son HY, Yang JH and Kim SH. 2008. Toxic effect of perfluo­rinated compounds. Report of Ministry of Food and Drug Safety.
  4. Underwood EJ. 1977. Trace element in human and animal nutri­tion. 4th ed., Academic, New York, U.S.A., 468.
  5. Christians O, Schreiber W and Manthey M. 1983. Krill process­ing and the fluoride problem. Proc. 6th Int. Conf, Food Sci Tech, 1, 167.
  6. Fluoride. 2010. Ministry of Food and Drug Safety.
  7. Dabeka RW and McKenzie AD. 1995. Survey of lead, cad­mium, fluoride, nickel, and cobalt in food composites and estimation of dietary intakes of these elements by Canadians in 1986-1988. J Assoc Off Anal Chem Int 78, 897-909.
  8. Chen YX, Lin MQ, He ZL, Chen C, Min D, Liu YQ and Yu MH. 1996. Relationship between total fluoride intake and dental fluorosis in areas polluted by airborne fluoride. Fluo­ride 29, 7-12.
  9. Sherlock JC. 1984. Fluorides in foodstuffs and the diet. The journal of the royal society for the promotion of health 104, 34-36. https://doi.org/10.1177/146642408410400110
  10. Kim KH, Kim DM and Kim YH. 1990. Fluoride reduction of antarctic krill by electrocondensation method. Korea J Food SCI Technol 22, 172-176
  11. Shivarajashankara YM, Shivashankara AR, Hanumanth RS and Gopalakrishna Bhat P. 2001. Oxidative stress in children with endemic skeletal fluorosis. Fluoride 34, 103.107.
  12. Xie CL, Kim HS, Shim KH, Yoon NY, Kim PH and Yoon HD. 2012. Organic acid extraction of fluoride from antarctic krill, Euphausia superba. Fish Aquat Sci 15, 203-207. http://dx.doi.org/10.5657/FAS.2012.0203.
  13. McCord JM and Frichvch I. 1969. Superoxide dismutase. An enzymic function for erythrocuprein(hemicuprein). J B Chem 244, 6049-6055.
  14. Halliwell B and Gutteridge GMC. 1981. Formation of thio­barbituric acid-reactive substance from deoxyribose in the persence of iron salts. FEBS Lett 128, 347-350. https://doi.org/10.1016/0014-5793(81)80114-7
  15. Oyanagui Y. 1984. Revaluation of assay methods and establish­ment of Kit for superoxide dismutase activith. Anal Bio­chem 42, 290-296.
  16. Rigo A and Rotilio G. 1977. Simultaneous determination of su­peroxide dismutase and catalase in biological materials by polarography. Anal Biochem 81, 157-166. https://doi.org/10.1016/0003-2697(77)90609-1
  17. Lawrence RA and Burk RF. 1978. Species, tissue and subcellu­lar distribution of non Se-de[endent glutathione peroxidase activity. Lipid 19, 444-452.
  18. Adelung D, Buchholz F, Culik B and Keck A. 1987. Fluoride in Tissues of Krill Euphausia superba Dana and Meganyc­tiphanes norvegica M. Sars in Relation to the Moult Cycle. Polar Biol 7, 43-50. https://doi.org/10.1007/BF00286823
  19. Whitford GM. 1996. The metabolism and toxicity of fluoride. Monographs in Oral Science 16, 1-53.
  20. Basha PM and Sujitha NS. 2012. Combined influence of inter­mittent exercise and temperature stress on the modulation of fluoride toxicity. Biol Trace Elem Res 148, 69-5. http:// dx.doi.org/10.1007/s12011-012-9338-4.
  21. Saralakumari D and Ramakrishna RP. 1991. Red cell membrane alteration in human chronic fluoride toxicity. Biochem Int 23, 639-648.
  22. Zhang C, Liu J, Ling B, Song X, Gu W and Wang G. 2000. The effect of fluoride-arsenic exposure on the lipid peroxi­dation and antioxidation of the off spring of rats. Zhonghua Yu Fang Yi Xue Za Zhi. 34, 134-5.
  23. Mittal M and Flora SJS. 2006. Effects of individual and com­bined exposure to superoxide dismutaseium arsenite and superoxide dismutaseium fluoride on tissue oxidative stress, arsenic and fluoride levels in male mice. Chem-Biol Interact 162, 128-139. https://doi.org/10.1016/j.cbi.2006.05.018
  24. Rzeuski R, Chlubek D and Machoy Z. 1998. Interactions be­tween fluoride and biological free radical reactions. Fluoride 31, 43-45.
  25. Chlubek D. 2003. Fluoride and oxidative stress. Fluoride 36, 217-228.
  26. Yamaguti PM, Simoes A, Ganzerla E, Souza DN, Nogueira FN and Nicolau J. 2013. Effects of Single Exposure of sodium Fluoride on Lipid Peroxidation and Antioxidant Enzymes in Salivary Glands of Rats. Oxid Med Cell Longevity 7.
  27. Chinoy NJ and Memon MR. 2001. Beneficial effects of some vitamins and calcium on fluoride and aluminium toxicity on gastrocnemius muscle and liver of male mice. Fluoride 34, 21-33.
  28. Ewa GM, Ewa B, Iwona B, Sławomir K, Tomasz W, El.bieta S and Barbara SP. 2009. The influence of superoxide dis­mutaseium fluoride and antioxidants on the concentration of malondialdehyde in rat blood plasma. Research report Fluo­ride 42, 101-104.
  29. Guo Z, Zhu Q, Hu C and Yang Y. 2002. Study on lipid peroxida­tion of electrolyzing-aluminum workers. Wei Sheng Yan Jin 31, 78-80.
  30. Trivedi,a MH, Trivedi,a RJ, Verma,b NP and Sangai,b NJ. 2011. Black tea extract mitigation of NaF-induced lipid peroxida­tion in different regions of mice brain. Research report Fluo­ride 4, 243-254.
  31. Stuart J. Newmana, Walter C. Dunlapb, Stephen Nicolc, David Ritza. 2000. Antarctic krill (Euphausia superba) acquire a UV-absorbing mycosporine-like amino acid from dietary al­gae. Journal of Experimental Marine Biology and Ecology 255, 93-110. https://doi.org/10.1016/S0022-0981(00)00293-8
  32. Yamada H, Ueda T, Yano A, 2011, Water-Soluble Extract of Pacific Krill Prevents Triglyceride Accumulation in Adi­pocytes by Suppressing PPARc and C/EBPa Expression. PLoS ONE 6. e21952. http://dx.doi.org/10.1371/journal. pone.0021952.