The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Fungicidal Effect of Slightly Acidic Hypochlorous Water against Phytopathogenic Fungi

미산성 차아염소산수의 식물병원균류에 대한 살균 효과

  • 송정영 (충남대학교 응용생물학과) ;
  • 김나래 (충남대학교 응용생물학과) ;
  • 남명현 (충남농업기술원 논산딸기시험장) ;
  • 박범진 (한국코스믹라운드(주)) ;
  • 황의일 ((주)케이티엔지 R&D본부 기술연구소) ;
  • 최종명 (충남대학교 원예학과) ;
  • 김홍기 (충남대학교 응용생물학과)
  • Received : 2013.11.15
  • Accepted : 2013.12.14
  • Published : 2013.12.30
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Abstract

Slightly acidic hypochlorous water (SAHW) is well known for having a powerful and broad spectrum antimicrobial activity, and is harmless to the environment and humans. SAHW (pH 5~6.5, 20~30 ppm available chlorine concentration) was generated by electrolysis of dilute solution of HCl (4%) in a chamber of a non-membrane electrolytic cell. Our objective was to determine SAHW has a potential fungicidal activity on some phytopathogenic fungi. Spores of Botrytis cinerea, Colletotrichum acutatum and Phytophthora capsici were not culturable on agar media at approximately 10 seconds after treatment by SAHW. However, inactivation of Penicillium hirsutum was required over 3 min. Dilution of SAHW with sterilized distilled water (SDW) at the ratio of 1:1 (SAHW:SDW) against C. acutatum showed 100% inactivation but, the efficacy in 1:2 decreased until 63.2%. Control value of SAHW was 70.4% against C. acutatum on pepper fruits when applied upto 24 h postinoculation. SAHW has a powerful and wide spectrum antifungal activity and could be applied as a potential alternative to fungicidal agent for control of plant disease.

미산성 차아염소산수는 다양한 종류의 미생물들에 대해 강력한 살균력을 나타내지만 인간과 자연에 무해한 살균제로 잘 알려져 있다. 4%의 희석된 염산만으로 무격막 전해조에서 전기분해를 통해 만들어졌으며 유효염소 농도가 20~30 ppm(HOCl 97%)이고 pH 5.0~6.5인 미산성 차아염소산수의 식물병원균들에 대한 살균효과를 조사하였다. Botrytis cinerea, Colletotrichum acutatum, Phytophthora capsici 등의 포자들에 대해서는 대략 10초 정도의 처리시간으로도 100% 살균효과가 나타났으나 Penicillium hirsutum의 경우는 3분 이상의 처리시간이 요구되었다. 희석농도에 따른 C. acutatum에 대한 살균효과를 조사한 결과 1:1(미산성 차아염소산수:멸균수)의 비율로 희석한 처리구에서는 포자발아가 100% 억제되었으나 1:2 처리구에서는 63%로 억제율이 낮아졌다. 고추탄저병균 C. acutatum을 고추열매에 접종한 후 24시간이 지나서 처리된 미산성 차아염소산수의 병방제 효과를 조사한 결과 방제가는 70.4%였다. 본 연구를 통해 미산성 차아염소산수의 강하고 광범위한 살균력이 확인되었으며 식물병 방제를 위한 살균제로 유용하게 활용될 수 있을 것으로 예상됐다.

Keywords

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