The Korean Journal of Food And Nutrition (한국식품영양학회지)

The Korean Society of Food and Nutrition (한국식품영양학회)
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Extractions of Surface-Active Substances from Defatted Rapeseed Meal (Brassica napus L.) by Supercritical Carbon Dioxide

초임계 CO2 유체 추출법을 이용한 탈지 유채박 중 표면활성물질 추출의 최적화

  • Kim, Jeong-Won (Dept. of Food Science & Technology, Chungnam National University) ;
  • Jeong, Yong-Seon (Dept. of Food Science & Technology, Chungnam National University) ;
  • Gil, Na-Young (Dept. of Food Science & Technology, Chungnam National University) ;
  • Lee, Eui-Seok (Dept. of Food Science & Technology, Chungnam National University) ;
  • Lee, Yong-Hwa (Bioenergy Crop Research Center, National Institute of Crop Science, RDA) ;
  • Jang, Young-Seok (Bioenergy Crop Research Center, National Institute of Crop Science, RDA) ;
  • Lee, Ki-Teak (Dept. of Food Science & Technology, Chungnam National University) ;
  • Hong, Soon-Taek (Dept. of Food Science & Technology, Chungnam National University)
  • 김정원 (충남대학교 식품공학과) ;
  • 정용선 (충남대학교 식품공학과) ;
  • 길나영 (충남대학교 식품공학과) ;
  • 이의석 (충남대학교 식품공학과) ;
  • 이영화 (농촌진흥청 국립식량과학원 바이오에너지작물센터) ;
  • 장영석 (농촌진흥청 국립식량과학원 바이오에너지작물센터) ;
  • 이기택 (충남대학교 식품공학과) ;
  • 홍순택 (충남대학교 식품공학과)
  • Received : 2013.10.28
  • Accepted : 2013.12.03
  • Published : 2013.12.31
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Abstract

In this study, an attempt is being made to extract surface-active substances from defatted rapeseed cakes by supercritical carbon dioxide fluid. Independent variables for the extraction process, being formulated by D-optimal design, are pressure (150~350 bar), temperature ($33{\sim}65^{\circ}C$ and co-solvent (ethanol, 50~250 g). The dependent variables of the extraction yield, the content of neutral lipids, phospholipids and glycolipids in the extracts were analyzed upon the results through the response surface methodology. As for the extraction yield, it was found to increase with increasing independent variables, among which the co-solvent proved to be a major influencing parameter. Similar trends were found for the content of surface-active substances (i.e, phospholipids and glycolipids) in the extracts, except for the content of neutral lipids. Regression equations were suggested to coincide well with the results from the experiments. Extraction conditions are being optimized to maximize the extraction yields, the content of phospholipids, and glycolipids were 350 bar (pressure), $65^{\circ}C$ (temperature) and 228.55 g (co-solvent), respectively.

본 실험에서는 탈지 유채박 중 표면활성물질의 추출을 위해 초임계 $CO_2$ 유체 추출법을 이용하였다. 추출 독립변수는 추출압력(150~350 bar), 온도($33{\sim}65^{\circ}C$), 보조용매량(ethanol, 150~250 g)으로 하였으며, D-optimal design에 의한 반응표면 분석을 통해 추출수율, 중성지질 분획, 인지질 분획, 당지질 분획 함량 등의 종속변수에 대한 최적 추출조건을 검토하였다. 그 결과, 압력, 온도, 그리고 보조용매량이 증가함에 따라 종속변수 즉, 추출수율, 인지질, 당지질 함량 등은 증가하였으나, 중성지질 함량은 감소하는 것으로 나타났으며, 그중 보조용매량이 각 종속변수에 가장 큰 영향을 주는 것으로 나타났다. 본 연구를 통해서 유도된 회귀식 모형은 실험을 통해 얻은 결과와 잘 일치하였으며, 초임계 $CO_2$ 유체 추출에 있어서 종속변수인 추출수율, 인지질, 당지질 함량 등을 최대로 하면서 중성지질 함량을 최소로 하는 최적화된 추출조건은 추출압력 350 bar, 추출온도 $65^{\circ}C$, 보조용매량 228.55 g으로 분석되었으며, 이 조건에서 추출수율은 5.98%, 추출물은 중성지질 3.0%, 인지질 69.43%, 당지질 17.46% 등의 조성을 나타낼 것으로 예측되었다.

Keywords

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