Korean Journal of Clinical Laboratory Science (대한임상검사과학회지)
- Volume 45 Issue 2
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- Pages.48-53
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- 2013
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- 1738-3544(pISSN)
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- 2288-1662(eISSN)
Species Identification of Nontuberculous Mycobacteria (NTM) by PCR-Restriction Fragment Length Polymorphism (PRA) of the rpoB Gene from Three Hospitals of Busan-Kyeongnam Area
- Choi, Sung-Ran (Department of Clinical Pathology, Student of Masan University) ;
- Kang, Min-Jung (Department of Clinical Pathology, Student of Masan University) ;
- Park, Gyu-Hwan (Department of Clinical Pathology, Student of Masan University) ;
- Kim, Da-Hye (Department of Clinical Pathology, Student of Masan University) ;
- Jeong, Da-Woon (Department of Clinical Pathology, Student of Masan University) ;
- Seo, Eun-Hye (Department of Clinical Pathology, Student of Masan University) ;
- Lee, Hyang-Min (Department of Clinical Pathology, Student of Masan University) ;
- Park, Hyun-Kyung (Department of Clinical Pathology, Student of Masan University) ;
- Jeong, Jin-Yee (Department of Clinical Pathology, Student of Masan University) ;
- Lee, Jung-Min (Department of Clinical Pathology, Student of Masan University) ;
- Jeong, Soo-Young (Department of Clinical Pathology, Student of Masan University) ;
- Lee, Jun-Young (Department of Clinical Pathology, Masan University) ;
- Cho, Eun-Jin (International Tuberculosis Reserch Center) ;
- Jekal, Suk (Goodmorning Hospital) ;
- Kim, Chung-Hwan (Department of Clinical Pathology, Masan University)
- Received : 2013.05.21
- Accepted : 2013.06.19
- Published : 2013.06.30
Abstract
Recently, the isolation rate of nontuberculous mycobacteria (NTM) in clinical laboratories and the incidence of NTM infections are on the increase in Korea, but there have been only a few studies that reveal the general aspect of NTM isolation or species distribution. Therefore, this study was performed to examine the species identification by PCR-restriction fragment length polymorphism analysis (PRA, PCR-RFLP), and the clinical significance of mycobacterial cultures. PRA was used during the novel region of the rpoB gene and was developed for rapid and precise identification of mycobacteria to the species level. From January 2012 to April 2012, we examined pre-identified nontuberculous mycobacteria (60 species in 3 hospital of Busan-Kyeongnam area). We confirmed 4 (6.6%) Mycobacterium tuberculosis (MTB) and 56 (93.4%) NTM from 60 pre-identified NTM species by multiplex PCR (MolecuTech