Journal of Korean Medicine for Obesity Research (한방비만학회지)

The Society of Korean Medicine for obesity Research (한방비만학회)
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Effects of Fermented Lotus Extracts on the Differentiation in 3T3-L1 Preadipocytes

3T3-L1 전지방세포에서 연잎-연근 혼합 발효물의 지방세포 분화 억제 효과

  • Lee, Sin Ji (Department of Oriental Rehabilitation Medicine, College of Oriental Medicine, Dongguk University) ;
  • Bose, Shambhunath (Department of Oriental Rehabilitation Medicine, College of Oriental Medicine, Dongguk University) ;
  • Lee, Su-Jin (Department of Oriental Rehabilitation Medicine, College of Oriental Medicine, Dongguk University) ;
  • Jeong, Ji-Eun (Department of Oriental Rehabilitation Medicine, College of Oriental Medicine, Dongguk University) ;
  • Koo, Byung-Soo (Department of Neuropsychiatry, College of Oriental Medicine, Dongguk University) ;
  • Kim, Dong-Il (Department of Obstetrics and Gynecology, College of Oriental Medicine, Dongguk University) ;
  • Kim, Hojun (Department of Oriental Rehabilitation Medicine, College of Oriental Medicine, Dongguk University)
  • 이신지 (동국대학교 한의과대학 한방재활의학과교실) ;
  • ;
  • 이수진 (동국대학교 한의과대학 한방재활의학과교실) ;
  • 정지은 (동국대학교 한의과대학 한방재활의학과교실) ;
  • 구병수 (동국대학교 한의과대학 한방신경정신과학교실) ;
  • 김동일 (동국대학교 한의과대학 한방부인과학교실) ;
  • 김호준 (동국대학교 한의과대학 한방재활의학과교실)
  • Received : 2013.11.11
  • Accepted : 2013.11.25
  • Published : 2013.12.30
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Abstract

Objectives: This study was performed to evaluate the effects of fermented lotus extracts on the inhibition of differentiation in 3T3-L1 preadipocytes. Methods: Extracts of lotus leaf and lotus root were fermented using 4 different probiotics separately, including Lactobacillus plantarum, Lactobacillus rhamnosus, Bifidobacterium breve, and Bifidobacterium longum. Inhibition of preadipocyte differentiation was examined by Oil red O dye staining. Expressions of adipogenic transcription factors including CCAAT/enhancer binding proteins (C/$EBP{\alpha}$) and peroxisome proliferators-activated receptor ${\gamma}$ ($PPAR{\gamma}$) were analyzed by real time polymerase chain reaction and Western blotting analysis. Results: Fermented lotus extracts inhibited adipogenic transcription factors by inhibiting preadipocytes differentiation. All of the groups fermented by 4 kinds of probiotics showed reduction in Oil Red O dye staining. Bifidobacterium breve showed the most effective inhibition of C/$EBP{\alpha}$. Bifidobacterium breve and Bifidobacterium longum showed the best downregulation of $PPAR{\gamma}$ expressions compared with the control and the unfermented lotus group. Conclusions: Fermented lotus extracts showed significant effects on inhibition of preadipocyte differentiation in 3T3-L1 preadipocytes showing correlation with insulin sensitivity and lipid metabolism related with obesity.

Keywords

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