Journal of fish pathology (한국어병학회지)

The Korean Society of Fish Pathology (한국어병학회)
권호 표지
DOI QR코드

DOI QR Code

Monitoring of Kudoa septempunctata in Cultured Olive Flounder and Wild Fish in Jeju Island during 2012

2012년 제주의 양식 넙치 및 자연산 어류의 Kudoa septempunctata 감염 조사

  • Song, Jun-Young (Pathology Division, National Fisheries Research & Development Institute(NFRDI)) ;
  • Choi, Jun-Ho (Pathology Division, National Fisheries Research & Development Institute(NFRDI)) ;
  • Choi, Hye-Sung (Pathology Division, National Fisheries Research & Development Institute(NFRDI)) ;
  • Jung, Sung Hee (Pathology Division, National Fisheries Research & Development Institute(NFRDI)) ;
  • Park, Myoung Ae (Aquatic Life Disease Control Division, NFRDI)
  • 송준영 (국립수산과학원 병리연구과) ;
  • 최준호 (국립수산과학원 병리연구과) ;
  • 최혜승 (국립수산과학원 병리연구과) ;
  • 정승희 (국립수산과학원 병리연구과) ;
  • 박명애 (국립수산과학원 수산생물방역과)
  • Received : 2013.10.04
  • Accepted : 2013.11.19
  • Published : 2013.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

The study surveyed infection rate of Kudoa septempunctata parasitized in the trunk muscle of olive flounder, Paralichthys olivaceus, cultured in Jeju Island and wild fish species caught in the coastal area around Jeju Island during 2012. Among 143 olive flounder that were randomly sampled from 26 different culture farms, K. septempunctata was detected in 7 fish samples (4.9%) from 4 different culture farms, showing no typical Kudoa infestation. However, K. septempunctata was not detected in olive flounder fry sampled from hatcheries and 8 species of wild fish. In addition, we compared 3 different sampling sites on trunk muscle of 7 Kudoa positive fish that included head part, tail part and entire muscle. Among 7 fish, K. septempunctata was detected in 3 fish that were sampled from head part; while 4 fish from tail part of trunk muscle. However, all 7 fish were positive when sampled from entire muscle. Thus, we suggest that it will be more efficient to use entire muscle sample than sampling partial muscle parts for detection of K. septempunctata.

Kudoa septempunctata가 한국의 양식 넙치의 근육에 기생하는 것이 보고되어, 본 연구에서는 2012년 동안 제주 지역의 양식 넙치 및 자연산 어류에서 K. septempunctata의 감염 현황을 조사하였다. 제주지역 26개소의 넙치 양식장으로부터 143마리를 조사한 결과, 4개소 7마리(4.9%)에서 쿠도아 감염이 확인되었으며, 넙치 치어 67마리에서는 쿠도아가 검출되지 않았다. 또한, 제주 지역에서 어획 된 자연산 어류 8종에서도 쿠도아가 검출되지 않았다. 쿠도아가 검출 된 7마리 어류에 대하여 등근육 부위별 쿠도아 검출을 비교하기 위하여, 넙치의 두부쪽에서 채취한 등근육, 미부쪽에서 채취한 등근육, 그리고 등근육전체를 긁어 모은 샘플을 사용하여 검출률을 비교한 결과, 전체를 긁어 모든 샘플을 사용할 경우 검출률이 100%로 가장 높아, 어느 한 쪽에서 근육을 채취하는 것 보다 등근육 전체 부위를 골고루 채취하는 것이 쿠도아 검출에 더 효율적임을 확인하였다.

Keywords

References

  1. Blaylock, R.B., Bullard, S.A., Whipps, C.M.: Kudoa Hypoepicardialis n. sp. (Myxozoa: Kudoidae) and associated lesions from the heart of seven perciform fishes in the northern gulf of Mexico. J. Parasitol., 90: 584-593, 2004. https://doi.org/10.1645/GE-161R
  2. Burger, M.A., Cribb, T.H., Adlard, R.D.: Patterns of relatedness in the Kudoidae with descriptions of Kudoa chaetodoni n. sp. and K. lethrini n. sp. (Myxosporea: Multivalvulida). Parasitology., 134: 669-681, 2007. https://doi.org/10.1017/S0031182006001995
  3. Egusa, S., Shiomitsu, T.: Two new species of the genus Kudoa (Myxosporea: Multivalvulida) from marine cultured fishes in Japan. Fish Pathol., 18: 163-171, 1983 https://doi.org/10.3147/jsfp.18.163
  4. Gilbert, M.A., Granath, Jr W.O.: Persistent infection of Myxobolus cerebralis, the causative agent of salmonid whirling disease, in Tubifex tubifex. J. Parasitol., 87: 101-107, 2001. https://doi.org/10.1645/0022-3395(2001)087[0101:PIOMCT]2.0.CO;2
  5. Grabner, D., Yokoyama, H., Shirakashi, S., Kinami, R.: Diagnostic PCR assays to detect and differentiate Kudoa septempunctata, K. thyrsites and K. lateolabracis (Myxozoa, Multivalvulida) in muscle tissue of olive flounder (Paralichthys olivaceus). Aquaculture., 338: 36-40, 2012.
  6. Harada, T., Kawai, T., Sato, H., Yokoyama, H., Kumeda, Y.: Development of a quantitative polymerase chain reaction assay for detection of Kudoa septempunctata in olive flounder (Paralichthys olivaceus). Int. J. Food Microbiol., 156: 161-167, 2012a. https://doi.org/10.1016/j.ijfoodmicro.2012.03.018
  7. Harada, T., Kawai, T., Jinnai, M., Ohnishi, T., Sugita-Konishi, Y., Kumeda, Y.: Detection of Kudoa septempunctata 18S ribosomal DNA in patient fecal samples from novel food-borne outbreaks caused by consumption of raw olive flounder (Paralichthys olivaceus). J. Clin. Microbiol., 50: 2964-2968, 2012b. https://doi.org/10.1128/JCM.01218-12
  8. Iijima, Y., Nakanishi, N., Furusawa, H., Ohnishi, T., Sugita-Konishi, Y.: Inter-Laboratory Validation and Applications of Quantitative Real-Time PCR for the Detection of Kudoa septempunctata in olive Flounder (Paralichthys olivaceus). Jpn. J. Infect. Dis., 65: 436-438, 2012. https://doi.org/10.7883/yoken.65.436
  9. Koie, M., Karlsbakk, E., Nylund, A.: The marine herring myxozoan Ceratomyxa auerbachi (Myxozoa: Ceratomyxidae) uses Chone infundibuliformis (Annelida: Polychaeta: Sabellidae) as invertebrate host. Folia Parasitol., 55: 100-104, 2008. https://doi.org/10.14411/fp.2008.013
  10. Kawai, T., Sekizuka, T., Yahata, Y., Kuroda, M., Kumeda, Y., Iijima, Y., Kamata, Y., Sugita-Konishi, Y., Ohnishi, T.: Identification of Kudoa septempunctata as the causative agent of novel food poisoning outbreaks in Japan by consumption of Paralichthys olivaceus in raw fish. Clin. Infect. Dis., 54: 1046-1052, 2012. https://doi.org/10.1093/cid/cir1040
  11. Kent, M.L., Andree, K.B., Bartholomew, J.L., El-Matbouli, M., Desser, SS., Devlin, R.H., Feist, S.W., Hedrick, R.P., Hoffmann, R.W., Khattra, J., Hallett, S.L., Lester, R.J., Longshaw, M., Palenzeula, O., Siddall, M.E., Xiao, C.: Recent Advances in Our Knowledge of the Myxozoa. J. Eukaryot. Microbiol., 48: 395-413, 2001. https://doi.org/10.1111/j.1550-7408.2001.tb00173.x
  12. Kudo, G., Barnett, H., Nelson, R.: Factors affecting cooked texture quality of Pacific whiting, Merluccius productus, fillets with particular emphasis on the effects of infection by the myxosporeans Kudoa paniformis and K. thyrsitis. Fish. Bull., 85: 745-756, 1987.
  13. Lom, J., Dykova, I.: Myxozoan genera: definition and notes on taxonomy, life-cycle terminology and pathogenic species. Folia Parasitol., 53: 1-36, 2006. https://doi.org/10.14411/fp.2006.001
  14. Maeno, Y., Nagasawa, K., Sorimachi, M.: Kudoa intestinalis n. sp. (Myxosporea: Multivalvulida) from the intestinal musculature of the striped mullet, Mugil cephalus, from Japan. J. Parasitol., 79: 190-192, 1993. https://doi.org/10.2307/3283506
  15. Martinez de Velasco, G., Rodero, M., Cuéllar, C., Chivato, T., Mateos, J., Laguna, R.: Skin prick test of Kudoa sp. antigens in patients with gastrointestinal and/or allergic symptoms related to fish ingestion. Parasitol. res., 103: 713-715, 2008. https://doi.org/10.1007/s00436-008-1017-1
  16. Matsukane, Y., Sato, H., Tanaka, S., Kamata, Y., Sugita-Konishi, Y.: Kudoa septempunctata n. sp. (Myxosporea: Multivalvulida) from an aquacultured olive flounder (Paralichthys olivaceus) imported from Korea. Parasitol. res., 107: 865-872, 2010. https://doi.org/10.1007/s00436-010-1941-8
  17. Moran, J.D.W., Whitaker, D.J., Kent, M.L.: A review of the myxosporean genus Kudoa Meglitsch, 1947, and its impact on the international aquaculture industry and commercial fisheries. Aquaculture., 172: 163-196, 1999. https://doi.org/10.1016/S0044-8486(98)00437-2
  18. Nakajima, K., Egusa, S.: Kudoa pericardialis n.sp. (Myxosporidea: Chloromyxidae) from cultured yellowtail. Seriola quinqueradiata Temminck et Schlegel. Bull. Jpn. Soc. Sci. Fish., 44: 117-120, 1978. https://doi.org/10.2331/suisan.44.117
  19. Sitja-Bobadilla, A.: Can Myxosporean parasites compromise fish and amphibian reproduction? Proceedings of the Royal Society B: Biological Sciences 276: 2861-2870, 2009. https://doi.org/10.1098/rspb.2009.0368
  20. Yokoyama, H.: A review: gaps in our knowledge on myxozoan parasites of fishes. Fish Pathol., 38: 125-136, 2003. https://doi.org/10.3147/jsfp.38.125
  21. Yokoyama, H., Whipps, C.M., Kent, M.L., Mizuno, K., Kawakami, H.: Kudoa thyrsites from Japanese flounder and Kudoa lateolabracis n. sp. from Chinese sea bass: causative myxozoans of post-mortem myoliquefaction. Fish Pathol., 39: 79-86, 2004. https://doi.org/10.3147/jsfp.39.79
  22. Zendt, J.S., Bergersen, E.P.: Distribution and abundance of the aquatic oligochaete host Tubifex tubifex for the salmonid whirling disease parasite Myxobolus cerebralis in the upper Colorado River basin. North American Journal of Fisheries Management 20: 502-512, 2000. https://doi.org/10.1577/1548-8675(2000)020<0502:DAAOTA>2.3.CO;2
  23. 일본 수산청 : 넙치에 기생하는 Kudoa septempunctata 의 검사법에 관하여, 2012.

Cited by

  1. Factors affecting sporoplasm release in Kudoa septempunctata vol.114, pp.2, 2015, https://doi.org/10.1007/s00436-014-4305-y
  2. Relationship between shipping amounts of olive flounder aquacultured from Jejudo and the reported events of acute food poisoning by Kudoa septempunctata in 2015, South Korea: an ecological study vol.39, 2017, https://doi.org/10.4178/epih.e2017041
  3. Stock assessment of starry flounder, Platichthys stellatus and olive flounder, Paralichthys olivaceus in the Uljin marine ranching area, Korea vol.50, pp.4, 2014, https://doi.org/10.3796/KSFT.2014.50.4.583
  4. Morphological and Molecular Comparisons between Japanese and Korean Isolates of Kudoa septempunctata (Myxozoa: Multivalvulida) in the Olive Flounder Paralichthys olivaceus vol.52, pp.3, 2017, https://doi.org/10.3147/jsfp.52.152
  5. Status of Marine Environment of Olive Flounder, Paralichythys olivaceus, Culture Ground in Jeju-do - Focus on Kudoa septempunctata positive and negative farm - vol.28, pp.3, 2016, https://doi.org/10.13000/JFMSE.2016.28.3.746
  6. Monitoring Kudoa septempunctata in Cultured Olive Flounder Paralichthys olivaceus in Different Regions of Korea in 2013 vol.47, pp.5, 2014, https://doi.org/10.5657/KFAS.2014.0611
  7. Analysis of Kudoa septempunctata as a cause of foodborne illness and its associated differential diagnosis vol.39, 2017, https://doi.org/10.4178/epih.e2017014
  8. A survey of Kudoa septempunctata in olive flounder (Paralichthys olivaceus) hatcheries in the southwestern coast of Korea between 2014 and 2015 vol.28, pp.2, 2015, https://doi.org/10.7847/jfp.2015.28.2.109
  9. A Distinguishing Staining Methods for Vitality of Kudoa (Kudoa septempunctata) spores in Muscle of Olive flounder (Paralichthys olivaceus) vol.28, pp.6, 2016, https://doi.org/10.13000/JFMSE.2016.28.6.1822
  10. Parvicapsula curvatura n. sp. in cultured olive flounder Paralichthys olivaceus and phylogenetic characteristics of the genus Parvicapsula vol.130, pp.3, 2018, https://doi.org/10.3354/dao03276
  11. Foodborne Illness Outbreaks in Gyeonggi Province, Korea, Following Seafood Consumption Potentially Caused by Kudoa septempunctata between 2015 and 2016 vol.9, pp.2, 2018, https://doi.org/10.24171/j.phrp.2018.9.2.05
  12. Monitoring of emaciation disease in cultured Paralichthys olivaceus of Jeju island during 2014-2015 vol.21, pp.6, 2013, https://doi.org/10.1186/s41240-018-0094-z
  13. Examination about Infection form of Kudoa septempunctata in muscle of olive flounder (Paralichthys olivaceus) using molecular and morphological methods vol.31, pp.1, 2013, https://doi.org/10.13000/jfmse.2019.2.31.1.154
  14. A new species Myxodavisia jejuensis n. sp. (Myxosporea: Sinuolineidae) isolated from cultured olive flounder Paralichthys olivaceus in South Korea vol.118, pp.11, 2013, https://doi.org/10.1007/s00436-019-06454-z
  15. Infections of Kudoa septempunctata (Myxozoa: Multivalvulida) in Wild Grass Puffer Takifugu alboplumbeus and Japanese Whiting Sillago japonica vol.56, pp.3, 2013, https://doi.org/10.3147/jsfp.56.140