DOI QR코드

DOI QR Code

Survey on Contamination of Fusarium Mycotoxins in 2011-harvested Rice and Its By-products from Rice Processing Complexes in Korea

미곡종합처리장에서 수집한 2011년산 쌀과 부산물의 Fusarium 곰팡이독소 오염 실태

  • Lee, Soohyung (Microbial Safety Division, National Academy of Agricultural Science-Rural Development Administration) ;
  • Lee, Theresa (Microbial Safety Division, National Academy of Agricultural Science-Rural Development Administration) ;
  • Kim, Mija (Microbial Safety Division, National Academy of Agricultural Science-Rural Development Administration) ;
  • Yu, Ohsuk (Microbial Safety Division, National Academy of Agricultural Science-Rural Development Administration) ;
  • Im, Hyunjin (Microbial Safety Division, National Academy of Agricultural Science-Rural Development Administration) ;
  • Ryu, Jae-Gee (Microbial Safety Division, National Academy of Agricultural Science-Rural Development Administration)
  • 이수형 (국립농업과학원 유해생물팀) ;
  • 이데레사 (국립농업과학원 유해생물팀) ;
  • 김미자 (국립농업과학원 유해생물팀) ;
  • 유오숙 (국립농업과학원 유해생물팀) ;
  • 임현진 (국립농업과학원 유해생물팀) ;
  • 류재기 (국립농업과학원 유해생물팀)
  • Received : 2013.11.04
  • Accepted : 2013.11.28
  • Published : 2013.12.31

Abstract

To investigate Fusarium mycotoxin contamination in rice samples from rice processing complexes (RPCs), paddy rice and rice-milling products such as husks, brown rice, blue-tinged rice, broken rice, rice bran, discolored rice, and polished rice were collected from nationwide in 2012. Three hundred seventy one samples of rice and its by-products were analyzed for three trichothethenes including nivalenol (NIV), deoxynivalenol (DON), and zearalenone (ZEA) by LC/MS. Discolored rice samples were found to have the highest contamination of DON, NIV or ZEA, followed by broken rice. Polished rice samples were largely free from mycotoxins, except three samples which were contaminated with NIV or DON at safety level. The rice byproduct samples were contaminated at higher level and frequencies than polished rice samples.

미곡종합처리장에서 수집한 2011년산 쌀 시료 중 Fusarium 곰팡이독소의 자연발생을 조사하기 위해서, 벼, 왕겨, 현미, 청치미, 싸라기, 미강, 색채미, 백미 등의 시료를 2012년에 전국적으로 수집하였다. 371점의 시료에 대해 DON, NIV과 ZEA의 자연발생을 LC/MS로 분석하였다. 이중 색채미가 오염율과 오염도가 가장 높았고 다음이 싸라기였다. 백미는 3점이 DON, NIV에 안전한 수준으로 오염되었었지만 나머지는 곰팡이독소가 검출되지 않았다. 부산물은 백미보다 오염도나 오염율이 높았다.

Keywords

References

  1. Codex Alimentarius Commission. 2006. Proposed draft maximum level for total aflatoxins in almonds, hazelnuts and pistachios "Ready-to-Eat". URL ftp://ftp.fao.org/codex/Reports/ Alinorm06/al29_12e.pdf
  2. Cunha, S. C. and Fernandes, J. O. 2010. Development and validation of a method based on a QuEChERS procedure and heart-cutting GC-MS for determination of five mycotoxins in cereal products. J. Sep Sci. 33: 600-609. https://doi.org/10.1002/jssc.200900695
  3. FAO. 2002. Post-harvest lossses: Discovering the full story. Chapter 3 - Survey and analysis of post-harvest losses. FAO Corporate Document Repository. URL http://www.fao.org/docrep/004/ac301e/AC301e04.htm
  4. Heidtmann-Bemvenuti, R., Hackbart, H. C. D., de Souza, M. M., Badiale-Furlong, E., Dors, G. C. and Fagundes, C. A. 2012. determination of deoxynivalenol and zearalenone in natural and parboiled rice and their fractions using quechers and Hplc/Uv-Fl. Quimica Nova. 35: 1244-1249. https://doi.org/10.1590/S0100-40422012000600033
  5. Japan Ministry of Agriculture Forestry and Fisheries. 2013. Survey on natural occurrence of cereal mycotoxins in Japan during 2002-2011. URL http://www.maff.go.jp/j/syouan/seisaku/risk_analysis/priority/kabidoku/tyosa/pdf/14_23.pdf
  6. Korea Food and Drug Administration. 2009. Regulatory impact analysis-control limit in foods. (In Korean)
  7. Lee, S. H., Son, S. W., Nam, Y. J., Shin, J. Y., Lee, S., Kim, M., Yun, J.-C., Ryu, J.-G. and Lee, T. 2010. Natural occurrence of Fusarium mycotoxins in field-collected maize and rice in Korea in 2009. Res. Plant Dis. 16: 306-311. (In Korean) https://doi.org/10.5423/RPD.2010.16.3.306
  8. Lee, T., Lee, S., Lee, S. H., Shin, J. Y., Yun, J.-C., Lee, Y. W. and Ryu, J.-G. 2011. Occurrence of Fusarium mycotoxins in rice and its milling by-products in Korea. J. Food Prot. 74: 1169-1174. https://doi.org/10.4315/0362-028X.JFP-10-564
  9. Lee, T., Lee, S., Lee, J.-H., Yun, J.-C. and Oh, K.-S. 2012. Natural occurrence of mycotoxin and fungi in Korean rice. Res. Plant Dis. 18: 261-267. (In Korean) https://doi.org/10.5423/RPD.2012.18.4.261
  10. Makun, H. A., Gbodi, T. A., Akanya, O. H., Salako, A. and Ogbadu, G. H. 2007. Fungi and some mycotoxins contaminating rice (Oryza sativa) in Niger State, Nigeria. African J. Biotechnol. 6: 99-108.
  11. Park, J. W., Choi, S. Y., Hwang, H. J. and Kim, Y. B. 2005. Fungal mycoflora and mycotoxins in Korean polished rice destined for humans. Int. J. Food Microbiol. 103: 305-314. https://doi.org/10.1016/j.ijfoodmicro.2005.02.001
  12. Park, J. W., Kim, E. K. and Kim, Y. B. 2004. Estimation of the daily exposure of Koreans to aflatoxin B1 through food consumption. Food Addit. Contam. 21: 70-75. https://doi.org/10.1080/02652030310001622782
  13. Paul, P. A., Lipps, P. E. and Madden, L. V. 2006. Meta-analysis of regression coefficients for the relationship between Fusarium head blight and deoxynivalenol content of wheat. Phytopathology 96: 951-961. https://doi.org/10.1094/PHYTO-96-0951
  14. Rasmussen, R. R., Storm, I., Rasmussen, P. H., Smedsgaard, J. and Nielsen, K. F. 2010. Multi-mycotoxin analysis of maize silage by LC-MS/MS. Anal. Bioanal. Chem. 397: 765-776. https://doi.org/10.1007/s00216-010-3545-7
  15. Reddy, K. R. N., Reddy, C. S., Abbas, H. K., Abel, C. A. and Muralidharan, K. 2008. Mycotoxigenic fungi, mycotoxins, and management of rice grains. Toxin Reviews 27: 287-317. https://doi.org/10.1080/15569540802432308
  16. Ryu, J.-G., Lee, S., Lee, S.-H., Son, S.-W., Nam, Y. J., Kim, M., Lee, T. and Yun, J.-C. 2011. Natural occurrence of Fusarium head blight and its mycotoxins in 2010-harvested barley and wheat grains in Korea. Res. Plant Dis. 17: 272-279. (In Korean) https://doi.org/10.5423/RPD.2011.17.3.272
  17. Sales, A. C. and Yoshizawa, T. 2005. Updated profile of aflatoxin and Aspergillus section Flavi contamination in rice and its byproducts from the Philippines. Food Addit. Contam. 22:429-436. https://doi.org/10.1080/02652030500058387
  18. Shim, H.-S., Hong, S.-G., Hong, S.-J., Kim, Y.-K., Ye, W.-H. and Sung, J.-M. 2005. Detection of the fungi associated with rice ear blight from rice seeds in Korea. Res. Plant Dis. 11: 93-97. (In Korean) https://doi.org/10.5423/RPD.2005.11.2.093
  19. Son, S. W., Nam, Y. J., Lee, S.-H., Lee, S. M., Lee, S., Kim, M., Lee, T., Yun, J.-C. and Ryu, J.-G. 2011. Toxigenic fungal contaminants in the 2009-harvested rice and its milling byproducts samples collected from rice processing complexes in Korea. Res. Plant Dis. 17: 280-287. (In Korean) https://doi.org/10.5423/RPD.2011.17.3.280
  20. Thirumala-Devi K., Mayo, M. A., Reddy, G. and Reddy, D. V. 2002. Occurrence of aflatoxins and ochratoxin A in Indian poultry feeds. J. Food Prot. 65: 1338-1340. https://doi.org/10.4315/0362-028X-65.8.1338
  21. Trebstein, A., Lauber, U. and Humpf, H. U. 2009. Analysis of Fusarium toxins via HPLC-MS/MS multimethods: matrix effects and strategies for compensation. Mycotoxin Res. 25:201-213. https://doi.org/10.1007/s12550-009-0029-8
  22. Turner, N. W., Subrahmanyam, S. and Piletsky, S. A. 2008. Analytical methods for determination of mycotoxins: A review. Analytical Chimica Acta 632: 168-180.
  23. Zacharaiasova, M., Lacina, O., Malachova, A., Kostelanska, M., Poustka, J., Godula, M. and Hajslova, J. 2010. Novel approaches in analysis of Fusarium mycotoxins in cereals employing ultra performance liquid chromatography coupled with high resolution mass spectrometry. Analytical Chimica Acta 662: 51-61. https://doi.org/10.1016/j.aca.2009.12.034

Cited by

  1. Occurrence of Fungi and Fusarium Mycotoxins in the Rice Samples from Rice Processing Complexes vol.20, pp.4, 2014, https://doi.org/10.5423/RPD.2014.20.4.289