Mass Spectrometry Letters

Korean Society for Mass Spectrometry (한국질량분석학회)
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Development of a Four-way Interface for Online Capillary Isoelectric Focusing-Electrospray-Mass Spectrometry (CIEF-ESI-MS)

  • Yu, Hai Dong (Division of Metrology for Quality of Life, Korea Research Institute of Standards and Science) ;
  • Kim, Byungjoo (Division of Metrology for Quality of Life, Korea Research Institute of Standards and Science) ;
  • Shin, Dae-Ho (Bio Medical Technologies Ltd.) ;
  • Ahn, Seonghee (Division of Metrology for Quality of Life, Korea Research Institute of Standards and Science)
  • Received : 2013.12.11
  • Accepted : 2013.12.19
  • Published : 2013.12.30
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Abstract

A new interface for coupling CIEF and MS using a four-way cross has been developed in a single mechanical system. This new interface could be operated without the electric discontinuity and reinstallation of lines. Additionally, a bare fused silica capillary was facilitated as a spray needle to produce electrospray and to guide catholyte or sheath liquid. Focusing for CIEF was completed in a hanging droplet at the end of spray needle. This capillary spray needle also provided stable spray, enhanced the ionization efficiency and increased sensitivity. Results with carbonic anhydrase I showed that focusing and spraying were well completed with the new interface and the new spray needle.

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References

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