Korean Journal of Poultry Science (한국가금학회지)

The Korean Society of Poultry Science (한국가금학회)
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Occurrence of Clostridium perfringens according to Raising Periods in Broilers

육계의 사육 일령별 Clostridium perfringens 발생 분석

  • Choe, Changyong (National Institute of Animal Science, RDA) ;
  • Park, In-Jae (Department of Infectious Diseases & Avian Diseases, College of Veterinary Medicine and Korea Zoonosis Research Institute, Jeonbuk National University) ;
  • Kang, Min (Department of Infectious Diseases & Avian Diseases, College of Veterinary Medicine and Korea Zoonosis Research Institute, Jeonbuk National University) ;
  • Jang, Hyung-Kwan (Department of Infectious Diseases & Avian Diseases, College of Veterinary Medicine and Korea Zoonosis Research Institute, Jeonbuk National University) ;
  • Hur, Tai-Young (National Institute of Animal Science, RDA) ;
  • Jung, Young-Hoon (National Institute of Animal Science, RDA) ;
  • Cho, Yong-Il (National Institute of Animal Science, RDA) ;
  • Do, Yoon-Jung (National Institute of Animal Science, RDA) ;
  • Yoo, Jae-Gyu (National Institute of Animal Science, RDA) ;
  • Na, Jae-Cheon (National Institute of Animal Science, RDA) ;
  • Hwangbo, Jong (National Institute of Animal Science, RDA)
  • 최창용 (농촌진흥청 국립축산과학원) ;
  • 박인재 (전북대학교 수의과대학.인수공통전염병연구소) ;
  • 강민 (전북대학교 수의과대학.인수공통전염병연구소) ;
  • 장형관 (전북대학교 수의과대학.인수공통전염병연구소) ;
  • 허태영 (농촌진흥청 국립축산과학원) ;
  • 정영훈 (농촌진흥청 국립축산과학원) ;
  • 조용일 (농촌진흥청 국립축산과학원) ;
  • 도윤정 (농촌진흥청 국립축산과학원) ;
  • 류재규 (농촌진흥청 국립축산과학원) ;
  • 나재천 (농촌진흥청 국립축산과학원) ;
  • 황보종 (농촌진흥청 국립축산과학원)
  • Received : 2013.10.14
  • Accepted : 2013.10.30
  • Published : 2013.12.30
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Abstract

The objective of this study was to investigate occurrence patterns of Clostridium perfringens on different raising periods in broilers. In different raising periods, we investigated the change in the gross lesion and microscopic histological findings of the mucose of the small intestine, colony forming unit (CFU) and the types C. perfringens with PCR assay. According to the gross lesions on the mucose of small intestine with 10-days-old broilers, the non-antibiotic group showed a higher value (0.6) than the antibiotic group (0.0). Whereas 20-days-old broilers with, the antibiotic treatment had a slightly lower value (1.0) than the non-antibiotic group (1.3). In the histological examination on the villi of the small intestine, there was no damage of the villi of the small intestine with 1-day-old broilers in both groups; however, the non-antibiotic group showed a higher value (0.4) than the antibiotic group (0.0) with 10-days-old broilers. In the non-antibiotic group, the CFU of C. perfringens of the fecal samples from the small intestine increased from 10 days of raising broilers and rapidly increase after 20 and 30 days of raising broilers. There was no detection of C. perfringens types with PCR assy in 1-day-old broilers, but we found C. perfringens type A in 10-, 20- and 30-days-old broilers. Although it is possible to raise healthy broilers by using antibiotics, the addition of antibiotics to concentrate feed is prohibited for public health. The results of this study would contribute to proper feeding management through the careful use of antibiotics.

본 연구는 육계(broiler)의 사육 일령에 따른 C. perfringens의 발생 양상을 확인하기 위해 실시하였는데, 사육일령별 소장 점막의 육안적(gross lesion) 변화, 현미경 검사를 통한 조직학적(histological) 소견을 분석하고, 배양한 분변의 C. perfringens CFU를 확인하며, PCR 검사를 통하여 C. perfringens type을 검색하였다. 소장 점막(mucose of small intestine)의 육안적 검사 결과, 10일령에서는 항생제를 첨가하지 않은 그룹에서 육안적 소견 0.6으로서 항생제 첨가군의 0.0에 비해 높은 수치를 나타내었으며, 20일령에서는 항생제 첨가군이 1.0으로서 항생제를 첨가하지 않았을 경우의 1.3보다 약간 낮은 경향을 나타내었다. 소장 융모(villi)의 조직학적 검사결과, 1일령에서 두 처리구에서 모두 소장 융모에 어떠한 손상도 나타내지 않다가 10일령에서는 항생제를 무첨가구에서 0.4를 나타낸 반면 항생제 첨가구에서는 0.0을 나타내어 항생제 첨가구의 소장이 손상을 받지 않은 것으로 나타났다. 소장 분변에서 C. perfringens의 CFU는 항생제를 사료에 첨가하지 않고 사육하였을 경우 10일령부터 증가하다가, 20일령 및 30일령에서는 그 수가 급격히 증가하는 것으로 나타났다. C. perfringens의 PCR 검사 결과, 1일령에는 두 처리구에서 모두 C. perfringens의 그 어떤 type도 검색되지 않았으나, 10일령, 20일령, 30일령에서 모두 type A의 ${\alpha}$-toxin이 검색되었다. 본 연구 결과, 육계의 사육 시 항생제 급여로 C. perfringens를 제어할 수 있지만, 배합사료 내 항생제 첨가가 금지된 만큼 수의사의 정확한 처방에 의한 선택적으로 항생제를 사용하면서 사양관리에 만전을 기해야 할 것으로 사료된다.

Keywords

References

  1. Al-Sheikhly F, Truscott RB 1977 The pathology of necrotic enteritis of chickens following infusion of crude toxins of Clostridium perfringens into the duodenum. Avian Dis 21: 241-255. https://doi.org/10.2307/1589344
  2. Asaoka Y, Yanai T, Hirayama H, Une Y, Saito E, Sakai H, Goryo M, Fukushi H, Maseqi T 2004 Fatal necrotic enteritis associated with Clostridium perfringens in wild crows (Corvus macrohynchos). Avian Pathol 33:19-24. https://doi.org/10.1080/03079450310001636228
  3. Barnes EM, Mead GC, Barnum DA, Harry EG 1972 The intestinal flora of the chicken in the period 2 to 6 weeks of age, with particular reference to the anaerobic bacteria. Br Poultry Sci 13:311-326. https://doi.org/10.1080/00071667208415953
  4. Chalmers G, Bruce HL, Hunter DB, Parreira VR, Kulkarni RR, Jiang YF, Prescott JF, Boerlin P 2008 Multilocus sequence typing analysis of Clostridium perfringens isolates from necrotic enteritis outbreaks in broiler chicken populations. J Clin Microbiol 46:3957-3964. https://doi.org/10.1128/JCM.01548-08
  5. Cooper KK, Gonger JG, Uzal FA 2013 Diagnosing clostridial enteric disease in poultry. J Vet Diagn Invest 25:314-327. https://doi.org/10.1177/1040638713483468
  6. Cravens RL, Goss GR, Chi F, De Boer ED, Davis SW, Hendrix SM, Richardson JA, Johnston SL 2013 The effects of necrotic enteritis, Aflatoxin B1 and virginiamycin on growth performance, necrotic enteritis lesion scores and mortality in young broilers. Poultry Sci 92:1997-2004. https://doi.org/10.3382/ps.2013-03011
  7. Droual R, Farver TB, Bickford AA 1995 Relationship of sex, age and cuncurrent intestinal disease to necrotic enteritis in turkeys. Avian Dis 39:599-605. https://doi.org/10.2307/1591814
  8. Elwinger K, Berndtson E, Engstrom B, Fossum O, Waldenstedt L 1998 Effect of antibiotic growth promoters and anticoccidials on growth of Clostridium perfringens in the caeca and on performance of broiler chickens. Acta Vet Scand 39:433-441.
  9. George BA, Quarles CL, Fagerberg DJ 1982 Virginiamycin effects on controlling necrotic enteritis infection in chickens. Poultry Sci 61:447-450. https://doi.org/10.3382/ps.0610447
  10. Gholamiandehkordi AR, Timbermont L, Lanckriet A, Van Den Broeck W, Pedersen K, Dewulf J, Pasmans F, Haesebrouck F, Ducatelle R, Van Immerseel F 2007 Quantification of gut lesions in subclinical necrotic enteritis model. Avian Pathol 36:375-382. https://doi.org/10.1080/03079450701589118
  11. Johansson A, Greko C, Engstrom BE, Karlsson M 2004 Antimicrobial susceptibility of Swedish, Norwegian and Danish isolates of Clostridium perfringens from poultry and distribution of tetracycline resistance genes. Vet Microbiol 99: 251-257 https://doi.org/10.1016/j.vetmic.2004.01.009
  12. Kaldhusdal M, Lovland A 2000 Necrotic enteritis: the economical impact of Clostridium perfringens is greater than anticipated. World Poultry 16:50-51.
  13. Knap I, Lund B, Kehlet AB, Hofacre C, Mathis G 2010 Bacillus licheniformis prevents necrotic enteritis in broiler chickens. Avian Dis 54:931-935. https://doi.org/10.1637/9106-101509-ResNote.1
  14. Knarreborg A, Simon MA, Engberg RM, Jensen BB, Tannock GW 2002 Effects of dietary fat source and subtherapeutic levels of antibiotic on the bacterial community in the ileum of broiler chickens at various ages. Appl Environ Microbiol 68:5918-5924. https://doi.org/10.1128/AEM.68.12.5918-5924.2002
  15. Kwon YK, Lee YJ, Mo IP 2004 An outbreak of necrotic enteritis in the ostrich farm in Korea. J Vet Med Sci 66: 1613-1615. https://doi.org/10.1292/jvms.66.1613
  16. Lanckriet A, Timbermont L, De Gussem M, Marien M, Vancraeynest D, Haesebrouck F, Ducatelle R, Van Immerseel F 2010 The effect of commonly used anticoccidials and antibiotics in a subclinical necrotic enteritis model. Avian Pathol 39:63-68. https://doi.org/10.1080/03079450903505771
  17. Long JR 1973 Necrotic enteritis in broiler. I. A review of the literature and the prevalence of the disease in Ontario. Can J Comp Med 37:302-308.
  18. Long JR, Pettit JR, Barnum DA 1974 Necrotic enteritis in broiler chickens. II. Pathology and proposed pathogenesis. Can J Comp Med 38:467-474.
  19. Lovland A, Kaldhusdal M, Redhead K, Skjerve E, Lillehaug A 2004 Maternal vaccination against subclicical necrotic enteritis in broilers. Avian Pathol 33:83-92.
  20. Lovland A, Kaldhusdal M, Reitan LJ 2003 Diagnosing Clostridium perfirngens associated necrotic enteritis in broiler flocks by an immunoglobulin G anti-alpha-toxin enzyme linked immunosorbent assay. Avian Pathol 32:527-534. https://doi.org/10.1080/0307945031000154134
  21. Martel A, Devriese LA, Cauwerts K, De Gussem K, Decostere A, Haesebrouck F 2004 Susceptibility of Clostridium perfringens strains from broiler chickens to antibiotics and anticoccidials. Avian Pathol 33:3-7. https://doi.org/10.1080/0307945031000163291
  22. McOrist S, Reece RL 1992 Clostridial enteritis in free-living lorikeets (Trichoglossus spp.). Avian Pathol 21:503-507. https://doi.org/10.1080/03079459208418868
  23. Olkowski AA, Wojnarowicz C, Chirino-Trejo M, Drew MD 2006 Responses of broiler chickens orally challenged with Clostridium perfringens isolated from field cases of necrotic enteritis. Res Vet Sci 81:99-108. https://doi.org/10.1016/j.rvsc.2005.10.006
  24. Parish WE 1961 Necrotic enteritis in the fowl (Gallus gallus domesticus). I. Histopathology of the disease and isolation of a strain of Clostridium welchii. J Comp Pathol 71:377-393. https://doi.org/10.1016/S0368-1742(61)80043-X
  25. Prescott JF, Sivendra R, Barnum DA 1978 The use of bacitracin in the prevention and treatment of experimentallyinduced necrotic enteritis in the chicken. Can Vet J 19: 181-183.
  26. Shane SM, Gyimah JE, Harrington KS, Snider TG 3rd 1985 Etiology and pathogenesis of necrotic enteritis. Vet Res Comun 9:269-287. https://doi.org/10.1007/BF02215151
  27. Slavic D, Boerlin P, Fabric M, Klotins KC, Zoethout JK, Weir PE, Bateman D 2011 Antimicrobial susceptibility of Clostridium perfringens isolates of bovine, chicken, porcine, and turkey origin from Ontario. Can J Vet Res 75: 89-97.
  28. Songer JG 1996 Clostridial enteric diseases of domestic animals. Clin Microbiol Rev 216:234.
  29. Stuve G, Hofshagen M, Holt G 1992 Necrotizing lesions in the intestine, gizzard and liver in captive capercaillies (Tetrao urogallus) associated with Clostridium perfringens. J Wildi Dis 28:598-602. https://doi.org/10.7589/0090-3558-28.4.598
  30. Van Immerseel F, De Buck J, Pasmans F, Huyghebaert G, Haesebrouck F, Ducatelle R 2004 Clostridium perfringens in poultry; an emerging threat for animal and public health. Avian Pathol 33:537-549. https://doi.org/10.1080/03079450400013162
  31. Watkins KL, Shryock TR, Dearth RN, Saif YM 1997 In-vitro antimicrobial susceptibility of Clostridium perfringens from commercial turkey and broiler ckicken origin. Vet Microbiol 54:195-200. https://doi.org/10.1016/S0378-1135(96)01276-X
  32. Wobeser G, Rainnie DJ 1987 Epizootic necrotic enteritis in wild geese. J Wildl Dis 23:376-385. https://doi.org/10.7589/0090-3558-23.3.376
  33. 신남식 권수완 이기환 김양범 최재훈 박주연 유한상 김대용 권오경 1999 바다사자에서 Clostridium perfringens 감염에 의한 괴사성 장염증례 보고. 한국임상수의학회지 16: 218-221.
  34. 장형관 2012 사료첨가용 항생제 사용금지로 인한 양계산업에 미치는 영향 및 대책방안. 대한수의사회지 48:104-108.
  35. 정희곤 1997 닭의 괴사성 장염, 새끼돼지 및 소의 장독혈증 에서 분리한 Clostridium perfringens의 S-R 변이와 항균 요법의 감수성. 한국식품영양학회지 10:166-173.

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