Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
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Anticancer Effects of the Extracts of Oriental Melon (Cucumis melo L. var makuwa Makino) Seeds

참외(Cucumis melo L. var makuwa Makino) 종자 추출물의 항암 활성

  • Kim, Jung-Hyun (Department of Horticultural Science, College of Agriculture and Life Sciences, Kyungpook National University) ;
  • Suh, Jun-Kyu (Department of Horticultural Science, College of Agriculture and Life Sciences, Kyungpook National University) ;
  • Kang, Young-Hwa (Department of Horticultural Science, College of Agriculture and Life Sciences, Kyungpook National University)
  • 김정현 (경북대학교 농업생명과학대학 원예과학과) ;
  • 서전규 (경북대학교 농업생명과학대학 원예과학과) ;
  • 강영화 (경북대학교 농업생명과학대학 원예과학과)
  • Received : 2012.10.23
  • Accepted : 2012.10.29
  • Published : 2012.10.29
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Abstract

The objective of this study was to investigate the anticancer effects of the extracts of oriental melon seeds. Various solvent extracts of oriental melon seeds were prepared and their anticancer effects were examined using in vitro MTT and CV assays. The anticancer effects of various extracts of oriental melon seeds were also examined in five human cancer cell lines including A549, AGS, HT-29, MCF-7 and HepG2. The ethanol extract of heated oriental melon seeds showed the potent cytotoxic effects especially against mouse hepatoma cell line(Hepa1c1c7), human hepatoma cell line(HepG2) and human breast cancer cell line(MCF-7). These data suggest that oriental melon seeds can be a promising anticancer agent against human liver and breast cancer.

참외 종자의 기능성을 규명하는 연구의 일환으로 항암활성을 조사하고자 하였다. 추출용매에 따른 항암활성을 조사하고자 헥산, 에탄올, 물 등 다양한 추출용매를 사용하여 참외 종자 추출물을 제조하였고, 가공에 따른 활성 증진효과를 알아보기 위해 볶음 처리하였다. 볶음 처리한 참외 종자는 볶음 처리하지 않은 종자보다 활성이 2~4배까지 증가하는 경향을 보여주었다. 추출용매에 따른 활성은 에탄올 추출물, 헥산 추출물, 물 추출물 순으로 항암활성이 나타났다. 인체 유래 5종(MCF-7, A549, AGS, HT-29, HepG2) 암 세포주를 이용하여 항암 활성의 조직 특이성을 알아보고자 하였다. 참외 볶음 종자 에탄올 추출물이 간암 세포주인 HepG2세포와 유방암 세포주인 MCF-7 세포에서 우수한 항암활성을 보여주었다. 용매분획법을 실시하여 제조한 분획물의 경우 에탄올 추출물보다 활성이 증가하였고, 비극성 분획물인 에칠아세테이트층이 극성 분획물인 부탄올층 보다 강한 항암활성을 보여주었다. 이상의 연구결과, 참외 볶음종자가 간암과 유방암 예방 및 치료 소재로서 개발될 가치가 있는 것으로 사료된다.

Keywords

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