Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
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Antioxidant Enzyme Activity and Antimicrobial Activity of Isatis tinctoria Extract

대청 추출물의 항산화 효소 활성 및 항균 효과

  • Heo, Buk-Gu (Naju Foundation of Natural Dyeing Culture) ;
  • Park, Yun-Jum (Department of Horticulture, Wonkwang University) ;
  • Lee, Seung-Jin (Naju Foundation of Natural Dyeing Culture) ;
  • Kim, Kwan-Su (Department of Oriental Medicine Resources, Mokpo National University) ;
  • Cho, Ja-Yong (Dept. of Medicated Diet & Food Technology, Namdo Provincial College of Jeonnam) ;
  • Boo, Hee-Ock (PHYTO M&F Co. Ltd., BI Center, Chosun University)
  • 허북구 ((재)나주시천연염색문화재단) ;
  • 박윤점 (원광대학교 원예학과) ;
  • 이승진 ((재)나주시천연염색문화재단) ;
  • 김관수 (목포대학교 자연과학대학 한약자원학과) ;
  • 조자용 (전남도립대학 약선식품가공과) ;
  • 부희옥 ((주)파이토M&F)
  • Received : 2012.05.19
  • Accepted : 2012.08.21
  • Published : 2012.10.29
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Abstract

To gather the basic data for increasing the utilization of Isatis tinctoria, we examined the effects of both antioxidative enzyme activity and antimicrobial activity from the extract of Isatis tinctoria. Ascorbate Peroxidase activities reveal that there is an decrease in order; ethanol extract from its stem (1601.7 Unit/mg protein), methanol extract from its leaf (1133.7 Unit/mg protein) and distilled water extract from its leaf (524.3 Unit/mg protein). Catalase activities reveal that there is an decrease in order; ethanol extract from its flower petal (177.1 Unit/mg protein), methanol extract from its leaf (120.8 Unit/mg protein) and distilled water extract from its flower petal (55.4 Unit/mg protein). Peroxidase activities reveal that there is an decrease in order; ethanol extract from its flower petal (27.1 Unit/mg protein), methanol extract from its flower petal (14.6 Unit/mg protein) and distilled water extract from its stem (10.4 Unit/mg protein). Superoxide dismutase activities reveal that there is an increase in order; distilled water extract from its root (90.8%), methanol extract from its flower petal (80.1%) and ethanol extract from its root (75.5%). Its flower extract showed a antimicrobial activity only against Vibrio parahaemolyticus, its root extract had only against Staphylococcus aureus, and its stem extract had against Bacillus subtilis, Escherichia coli and Staphylococcus aureus, regardless of solvents. Especially, distilled water extract from its leaf showed a high antimicrobial activity against both Bacillus subtilis and Escherichia coli and inhibition diameters against those were 30.0 and 24.0 mm, respectively.

대청의 이용성 향상을 위한 자료 확보 측면에서 식물체 부위 및 추출 용매별에 따른 항산화 효소 활성 및 항균효과를 조사하였다. APX(Ascorbate Peroxidase) 활성은 줄기의 에탄올 추출물, 잎의 메탄올 추출물, 잎의 증류수의 추출물 순으로 높아 각각 1601.7, 1133.7 및 524.3(Unit/mg protein)을 나타냈다. CAT(Catalase) 활성은 꽃의 에탄올 추출물, 잎의 메탄올 추출물, 꽃의 증류수 추출물 순으로 높았는데, 각각 177.1, 120.8 및 55.4(Unit/mg protein)를 나타냈다. POD(Ascorbate Peroxidase) 활성은 꽃의 에탄올 추출물, 꽃의 메탄올 추출물, 줄기의 증류수 추출물 순으로 높았으며, 각각 27.1, 14.6 및 10.4(Unit/mg protein)를 나타냈다. SOD(superoxide dismutase) 활성은 뿌리의 증류수 추출물, 꽃의 메탄올 추출물, 뿌리의 에탄올 추출물 순으로 높았으며, 각각 90.8, 80.1, 75.5%를 나타냈다. 대청의 꽃 추출물은 Vibrio parahaemolyticus에 대해서만, 뿌리는 Staphy lococcus aureus에 대해서만, 줄기 추출물은 Bacillus subtilis, Escherichia coli, Staphy lococcus aureus에 대해서만 용매에 관계없이 항균활성을 나타냈다. 특히 대청 잎의 증류수 추출물은 Bacillus subtilis, Escherichia coli에 대해 높은 항균활성을 나타내어 저해환 직경이 각각 30.0 및 24.0 mm이었다. 이와 같은 결과는 약용식물로서 대청의 가치가 높음을 시사해 주었다.

Keywords

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